Sulforaphane protects ischemic injury of hearts through antioxidant pathway and mitochondrial KATP channels

Piao, Cheng Shi; Gao, Shan; Lee, Geum-Hwa; Kim, Do Sung; Park, Byung‐Hyun; Chae, Soo Wan; Chae, Han-Jung; Kim, Suhn Hee

doi:10.1016/j.phrs.2009.11.009

Cited by 110 publications

(73 citation statements)

References 30 publications

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“…In addition, we further demonstrated that the cardioprotective effects of SFN depended on the activation of SIRT1 signaling pathway, which was shown to suppress ER stress and cardiomyocytes apoptosis in cardiomyocytes exposed to H/R. SFN, an isothiocyanate that is present in cruciferous vegetables, has been shown to exert beneficial preventive effect for cardiovascular diseases [9,24,25] . However, the mechanism still remains unclear.…”

Section: Discussionmentioning

confidence: 73%

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Sulforaphane prevents rat cardiomyocytes from hypoxia/reoxygenation injury in vitro via activating SIRT1 and subsequently inhibiting ER stress

et al. 2016

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Aim: Sulforaphane (SFN), a natural dietary isothiocyanate, is found to exert beneficial effects for cardiovascular diseases. This study aimed to investigate the mechanisms underlying the protective effects of SFN in a model of myocardial hypoxia/reoxygenation (H/R) injury in vitro. Methods: Cultured neonatal rat cardiomyocytes pretreated with SFN were subjected to 3-h hypoxia followed by 3-h reoxygenation. Cell viability and apoptosis were detected. Caspase-3 activity and mitochondrial membrane potential (ΔΨm) was measured. The expression of ER stress-related apoptotic proteins were analyzed with Western blot analyses. Silent information regulator 1 (SIRT1) activity was determined with SIRT1 deacetylase fluorometric assay kit. Results: SFN (0.1-5 μmol/L) dose-dependently improved the viability of cardiomyocytes, diminished apoptotic cells and suppressed caspase-3 activity. Meanwhile, SFN significantly alleviated the damage of ΔΨm and decreased the expression of ER stress-related apoptosis proteins (GRP78, CHOP and caspase-12), elevating the expression of SIRT1 and Bcl-2/Bax ratio in the cardiomyocytes. Co-treatment of the cardiomyocytes with the SIRT1-specific inhibitor Ex-527 (1 μmol/L) blocked the SFN-induced cardioprotective effects. Conclusion: SFN prevents cardiomyocytes from H/R injury in vitro most likely via activating SIRT1 pathway and subsequently inhibiting the ER stress-dependent apoptosis.

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Section: Discussionmentioning

confidence: 73%

“…Moreover, there have been additional studies implicating the cardioprotective role of SFN [6] . SFN has been proven to protect neonatal rat cardiomyocytes from oxidative stress in vitro [7,8] and to blunt postischemic cardiac injury in vivo [9] . However, the mechanism involved in the cardioprotective role of SFN is still unclear.…”

Section: Introductionmentioning

confidence: 99%

Sulforaphane prevents rat cardiomyocytes from hypoxia/reoxygenation injury in vitro via activating SIRT1 and subsequently inhibiting ER stress

et al. 2016

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“…Calcium/calmodulin-dependent protein kinase II inhibition prevented apoptosis by increasing the Bcl-2/Bax ratio and reducing caspase-3 activity in isolated cardiomyocytes [30]. Similarly, sulforaphane inhibited apoptosis by elevating the Bcl-2/Bax ratio and decreasing caspase-3 in isolated rat hearts [31]. Apoptosis is mediated by an activation cascade of the caspase family of cysteine proteases and ends with activation of the terminal effector caspase, caspase-3 [32].…”

Section: Discussionmentioning

confidence: 99%

The Protective Effect of Epigallocatechin-3 Gallate on Ischemia/Reperfusion Injury in Isolated Rat Hearts: Anex vivoApproach

Piao

Kim

et al. 2011

Korean J Physiol Pharmacol

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The aim of this study was to evaluate the preventive role of epigallocatechin-3 gallate (EGCG, a derivative of green tea) in ischemia/reperfusion (I/R) injury of isolated rat hearts. It has been suggested that EGCG has beneficial health effects, including prevention of cancer and heart disease, and it is also a potent antioxidant. Rat hearts were subjected to 20 min of normoxia, 20 min of zero-flow ischemia and then 50 min of reperfusion. EGCG was perfused 10 min before ischemia and during the whole reperfusion period. EGCG significantly increased left ventricular developed pressure (LVDP) and increased maximum positive and negative dP/dt (＋ /-dP/dtmax). EGCG also significantly increased the coronary flow (CF) at baseline before ischemia and at the onset of the reperfusion period. Moreover, EGCG decreased left ventricular end diastolic pressure (LVEDP). This study showed that lipid peroxydation was inhibited and Mn-SOD and catalase expressions were increased in the presence of EGCG. In addition, EGCG increased levels of Bcl-2, Mn-superoxide dismutase (SOD), and catalase expression and decreased levels of Bax and increased the ratio of Bcl-2/Bax in isolated rat hearts. Cleaved caspase-3 was decreased after EGCG treatment. EGCG markedly decreased the infarct size while attenuating the increase in lactate dehydrogenase (LDH) levels in the effluent. In summary, we suggest that EGCG has a protective effect on I/R-associated hemodynamic alteration and injury by acting as an antioxidant and anti-apoptotic agent in one.

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“…In this model, curcumin (Morimoto et al, 2008), a broccoli diet (Mukherjee et al, 2010), and sulforaphane administration (Piao et al, 2010) were found to inhibit myocardial hypertrophy, improve systolic function, and reduce myocardial infarction.…”

Section: Models For Studies On Prevention Of Cvdmentioning

confidence: 95%

Plant Science and Human Nutrition: Challenges in Assessing Health-Promoting Properties of Phytochemicals

Τράκα

Mithen

2011

Plant Cell

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The rise in noncommunicable chronic diseases associated with changing diet and lifestyles throughout the world is a major challenge for society. It is possible that certain dietary components within plants have roles both in reducing the incidence and progression of these diseases. We critically review the types of evidence used to support the health promoting activities of certain phytochemicals and plant-based foods and summarize the major contributions but also the limitations of epidemiological and observational studies and research with the use of cell and animal models. We stress the need for human intervention studies to provide high-quality evidence for health benefits of dietary components derived from plants.

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Sulforaphane protects ischemic injury of hearts through antioxidant pathway and mitochondrial KATP channels

Cited by 110 publications

References 30 publications

Sulforaphane prevents rat cardiomyocytes from hypoxia/reoxygenation injury in vitro via activating SIRT1 and subsequently inhibiting ER stress

Sulforaphane prevents rat cardiomyocytes from hypoxia/reoxygenation injury in vitro via activating SIRT1 and subsequently inhibiting ER stress

The Protective Effect of Epigallocatechin-3 Gallate on Ischemia/Reperfusion Injury in Isolated Rat Hearts: Anex vivoApproach

Plant Science and Human Nutrition: Challenges in Assessing Health-Promoting Properties of Phytochemicals

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