Sulforaphane Inhibits the Proliferation of the BIU87 Bladder Cancer Cell Line via IGFBP-3 Elevation

Dang, Yamei; Huang, Gang; Chen, Yirong; Dang, Zhong-Feng; Chen, Cheng; Liu, Feng‐Lei; Guo, Yanxia; Xie, Xiaodong

doi:10.7314/apjcp.2014.15.4.1517

Cited by 19 publications

(16 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SFN induced G2/M phase-arrest has also been observed in osteosarcoma [15], bladder [16], colon [17], ovarian [18], prostate [19], and breast cancer cells [20]. This G2/M phase-arrest corroborates the SFN induced decrease in RCC proliferation found in the present investigation.…”

Section: Discussionsupporting

confidence: 89%

Sulforaphane inhibits proliferation and invasive activity of everolimus-resistant kidney cancer cells in vitro

et al. 2016

View full text Add to dashboard Cite

Although the mechanistic target of rapamycin (mTOR) inhibitor, everolimus, has improved the outcome of patients with renal cell carcinoma (RCC), improvement is temporary due to the development of drug resistance. Since many patients encountering resistance turn to alternative/complementary treatment options, an investigation was initiated to evaluate whether the natural compound, sulforaphane (SFN), influences growth and invasive activity of everolimus-resistant (RCCres) compared to everolimus-sensitive (RCCpar) RCC cell lines in vitro. RCC cells were exposed to different concentrations of SFN and cell growth, cell proliferation, apoptosis, cell cycle, cell cycle regulating proteins, the mTOR-akt signaling axis, adhesion to human vascular endothelium and immobilized collagen, chemotactic activity, and influence on surface integrin receptor expression were investigated. SFN caused a significant reduction in both RCCres and RCCpar cell growth and proliferation, which correlated with an elevation in G2/M- and S-phase cells. SFN induced a marked decrease in the cell cycle activating proteins cdk1 and cyclin B and siRNA knock-down of cdk1 and cyclin B resulted in significantly diminished RCC cell growth. SFN also modulated adhesion and chemotaxis, which was associated with reduced expression of the integrin subtypes α5, α6, and β4. Distinct differences were seen in RCCres adhesion and chemotaxis (diminished by SFN) and RCCpar adhesion (enhanced by SFN) and chemotaxis (not influenced by SFN). Functional blocking of integrin subtypes demonstrated divergent action on RCC binding and invasion, depending on RCC cell sensitivity to everolimus. Therefore, SFN administration could hold potential for treating RCC patients with established resistance towards everolimus.

show abstract

Section: Discussionsupporting

confidence: 89%

Sulforaphane inhibits proliferation and invasive activity of everolimus-resistant kidney cancer cells in vitro

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In the same study extending to an in vivo model, oral administration of sulforaphane to the ALL xenograft models resulted in a significant reduction of tumor burden. Sulforaphane could also suppress the proliferation of bladder cancer BIU87 cells with an 80 μM dose leading to the inhibition of cell proliferation, induction of apoptosis and cell cycle arrest at the G2/M phase and down regulation of pro-inflammatory NF-kB expression [52]. In a murine UMUC3 invasive bladder cell xenograft model, feeding with semi-purified diets containing four percent broccoli sprouts or two percent broccoli sprout isothiocyanate extract, or gavage of pure sulforaphane (at 295 μmol/kg; similar to dietary exposure) was reported to have resulted in tumor weight reduction by 42%, 33% and 58%, respectively [53].…”

Section: Anticancer Action Mechanisms Of Sulforaphanementioning

confidence: 99%

Sulforaphane (SFN): An Isothiocyanate in a Cancer Chemoprevention Paradigm

Ullah¹

2015

Medicines

View full text Add to dashboard Cite

The International Agency for Research on Cancer (IARC) in its latest World Cancer Report (2014) has projected the increase in the global cancer burden from 14 million (2012) to 22 million incidence annually within the next two decades. Such statistics warrant a collaborative engagement of conventional and complementary and alternative therapies to contain and manage cancer. In recent years, there has been a shift in the cancer chemoprevention paradigm with a significant focus turning towards bioactive components of human diets for their anticancer properties. Since diet is an integral part of lifestyle and given that an estimated one third of human cancers are believed to be preventable though appropriate lifestyle modification including dietary habits, the current shift in the conventional paradigm assumes significance. Several epidemiological studies have indicated that consumption of broccoli is associated with a lower risk of cancer incidence including breast, prostate, lung, stomach and colon cancer. The edible plant belonging to the family of cruciferae such as broccoli is a rich source of glucoraphanin, a precursor of isothiocyanate sulforaphane which is considered to be a potent anti-cancer agent. Plant-based dietary agents such as sulforaphane mimic chemotherapeutic drugs such as vorinostat, possessing histone deacetylase inhibition activity. Evidence from epidemiological and experimental studies have emerged, enhancing the clinical plausibility and translational value of sulforaphane in cancer chemoprevention. The present review provides the current understanding of the cancer chemopreventive pharmacology of sulforaphane towards its potential as an anticancer agent.

show abstract

“…Also, SFN has been shown to be selectively more toxic to malignant urothelial cells (human) than normal urothelium [68]. Using BIU87 bladder cell lines Dang et al demonstrated that SFN downregulated NF-KB levels and upregulated Insulin-like growth factor-binding protein-3 (IGFBP-3) resulting in more apoptosis [99]. Islam et al, studied combination of SFN with acetazolamide in bladder cancer in vitro cell lines and xenografts.…”

Section: Resultsmentioning

confidence: 99%

Sulforaphane for the chemoprevention of bladder cancer: molecular mechanism targeted approach

et al. 2017

View full text Add to dashboard Cite

The clinical course for both early and late stage Bladder Cancer (BC) continues to be characterized by significant patient burden due to numerous occurrences and recurrences requiring frequent surveillance strategies, intravesical drug therapies, and even more aggressive treatments in patients with locally advanced or metastatic disease. For these reasons, BC is also the most expensive cancer to treat. Fortunately, BC offers an excellent platform for chemoprevention interventions with potential to optimize the systemic and local exposure of promising agents to the bladder mucosa. However, other than smoking cessation, there is a paucity of research that systematically examines agents for chemoprevention of bladder cancers. Adopting a systematic, molecular-mechanism based approach, the goal of this review is to summarize epidemiological, in vitro, and preclinical studies, including data regarding the safety, bioavailability, and efficacy of agents evaluated for bladder cancer chemoprevention. Based on the available studies, phytochemicals, specifically isothiocyanates such as sulforaphane, present in Brassicaceae or “cruciferous” vegetables in the precursor form of glucoraphanin are: (a) available in standardized formulations; (b) bioavailable- both systemically and in the bladder; (c) observed to be potent inhibitors of BC carcinogenesis through multiple mechanisms; and (d) without toxicities at these doses. Based on available evidence from epidemiological, in vitro, preclinical, and early phase trials, phytochemicals, specifically isothiocyanates (ITCs) such as sulforaphane (SFN) represent a promising potential chemopreventitive agent in bladder cancer.

show abstract

Sulforaphane Inhibits the Proliferation of the BIU87 Bladder Cancer Cell Line via IGFBP-3 Elevation

Cited by 19 publications

References 19 publications

Sulforaphane inhibits proliferation and invasive activity of everolimus-resistant kidney cancer cells in vitro

Sulforaphane inhibits proliferation and invasive activity of everolimus-resistant kidney cancer cells in vitro

Sulforaphane (SFN): An Isothiocyanate in a Cancer Chemoprevention Paradigm

Sulforaphane for the chemoprevention of bladder cancer: molecular mechanism targeted approach

Contact Info

Product

Resources

About