Sulforaphane inhibits 4-aminobiphenyl-induced DNA damage in bladder cells and tissues

Simultaneous Targeting of Bladder Tumor Growth, Survival, and Epithelial-to-Mesenchymal Transition with a Novel Therapeutic Combination of Acetazolamide (AZ) and Sulforaphane (SFN)

“…Recently, it was shown that SFN inhibits the growth of the ovarian cancer cells through the Akt mediated signalling pathway [51] and induces cell cycle inhibition in the Go/G1 phase in bladder cancer [19]. SFN can inhibit 4-aminobiphenyl-induced DNA damage in RT4 bladder cancer cells and in mouse bladder tissue [52], and in a separate study, showed inhibition of survivin, EGFR, and G2/M cell cycle arrest after a sulforaphane plus erucin combination treatment [53]. Although cell cycle arrest at G2M has been frequently observed after treatment with SFN, arrest at other phases of cell cycle has also been reported for many cell lines.…”

Animal Models for Basic and Preclinical Research in Bladder Cancer

“…They concluded that rodent models (in OH-BBN-treated B6D2F1 mice and Fischer-344 rats) of bladder cancer accurately represent the clinical situation to an extent that will allow successful miming of target genes, showing that these models are powerful tools for chemoprevention research (Lu et al, 2010). Using this experimental model, it has been demonstrated that NSAIDs (such as indomethacin, naproxen, NO-naproxen, and celecoxib), various EGFR inhibitors, and purified natural compounds (such as tea polyphenols and sulforaphane) have striking efficacy to prevent bladder tumor development (Ding et al, 2010;Grubbs et al, 2000;Lubet et al, 2005;Steele et al, 2009;Yao et al, 2004). Two disadvantages inherent in these models are the long experimental times (usually periods between 8 to 12 months) and the occupational exposure of workers.…”

Natural sulforaphane as a functional chemopreventive agent: including a review of isolation, purification and analysis methods

“…The tumor-inhibitory activity by the compound is linked with the activation of activator protein and the induction of apoptosis in lung tissues, suggesting that SF may also inhibit the development of adenomas to adenocarcinomas in the lung (Conaway et al, 2005). Ding et al (2010) showed that SF was highly effective in blocking aminobiphenyl-induced DNA damage in human bladder cells in vitro and in mouse bladder tissue in vivo. UV-radiation-induced skin carcinogenesis in 'initiated high-risk mice' was substantially inhibited by broccoli sprout extracts containing SF .…”