Sulforaphane attenuates 5-fluorouracil induced intestinal injury in mice

Wei, Liyang; Wang, Jinjin; Yan, Ling; Shui, Shanshan; Wang, Lei; Zheng, Wenxiu; Liu, Shuai; Liu, Changhong

doi:10.1016/j.jff.2020.103965

Cited by 16 publications

(11 citation statements)

References 82 publications

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“…The administration of SFN attenuated the severity of intestinal injury by showing significant improvement in weight, intestinal inflammation and intestinal permeability in mice. On the other hand, the SFN intake did not show any significant changes in SCFAs levels that were reduced previously due to 5-Fluorouracil treatment [ 98 ]. These studies may imply administration of BSp has a minor effect on the SCFAs profile, although that might be attributed to the characteristics of the experimental mice and could presumably reflect the complexity of interactions between the several bacterial species residing in the gut of these mice.…”

Section: Discussionmentioning

confidence: 71%

Nutritional combinatorial impact on the gut microbiota and plasma short-chain fatty acids levels in the prevention of mammary cancer in Her2/neu estrogen receptor-negative transgenic mice

et al. 2020

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Breast cancer is the second leading cause of cancer-related mortality in women. Various nutritional compounds possess anti-carcinogenic properties which may be mediated through their effects on the gut microbiota and its production of short-chain fatty acids (SCFAs) for the prevention of breast cancer. We evaluated the impact of broccoli sprouts (BSp), green tea polyphenols (GTPs) and their combination on the gut microbiota and SCFAs metabolism from the microbiota in Her2/neu transgenic mice that spontaneously develop estrogen receptor-negative [ER(-)] mammary tumors. The mice were grouped based on the dietary treatment: control, BSp, GTPs or their combination from beginning in early life (BE) or life-long from conception (LC). We found that the combination group showed the strongest inhibiting effect on tumor growth volume and a significant increase in tumor latency. BSp treatment was integrally more efficacious than the GTPs group when compared to the control group. There was similar clustering of microbiota of BSp-fed mice with combination-fed mice, and GTPs-fed mice with control-fed mice at pre-tumor in the BE group and at pre-tumor and post-tumor in the LC group. The mice on all dietary treatment groups incurred a significant increase of Adlercreutzia, Lactobacillus genus and Lachnospiraceae, S24-7 family in the both BE and LC groups. We found no change in SCFAs levels in the plasma of BSp-fed, GTPs-fed and combination-fed mice of the BE group. Marked changes were observed in the mice of the LC group consisting of significant increases in propionate and isobutyrate in GTPs-fed and combination-fed mice. These studies indicate that nutrients such as BSp and GTPs differentially affect the gut microbial composition in both the BE and LC groups and the key metabolites (SCFAs) levels in the LC group. The findings also suggest that temporal factors related to different time windows of consumption during the life-span can have a promising influence on the gut microbial composition, SCFAs profiles and ER(-) breast cancer prevention.

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Section: Discussionmentioning

confidence: 71%

Nutritional combinatorial impact on the gut microbiota and plasma short-chain fatty acids levels in the prevention of mammary cancer in Her2/neu estrogen receptor-negative transgenic mice

et al. 2020

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“…To investigate the antioxidant pathway, a further positive control group was used with sulforaphane (SF), an activator of Nrf2, dissolved in salad oil at 20 mg/kg as previously described [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

PEA/Polydatin: Anti-Inflammatory and Antioxidant Approach to Counteract DNBS-Induced Colitis

et al. 2021

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Palmitoylethanolamide (PEA) has well-known anti-inflammatory effects. However, PEA does not possess an antioxidant ability. A comicronized formulation of ultramicronized PEA (um-PEA) and polydatin (Pol) PEA/Pol, a biological precursor of resveratrol with antioxidant activity, could have protective effects on oxidative stress produced by inflammatory processes. We evaluated the effects of a comicronized PEA/Pol 10 mg/kg (9 mg of um-PEA+1 mg of polydatin) in a model of Dinitrobenzene sulfonic acid (DNBS)-induced colitis. Ulcerative colitis was induced in mice by intrarectally injection of DNBS (4 mg in 100 µL of 50% ethanol per mouse). Macroscopic and histologic colon alterations and marked clinical signs were observed four days after DNBS and elevated cytokine production. The myeloperoxidase (MPO) activity assessed for neutrophil infiltration was associated with ICAM-1 and P-selectin adhesion controls in colons. Oxidative stress was detected with increased poly ADP-ribose polymerase (PARP) and nitrotyrosine positive staining and malondialdehyde (MDA) levels in inflamed colons. Macroscopic and histologic alterations minimized by oral PEA/Pol, as well as neutrophil infiltration, inflammatory cytokine release, MDA, nitrotyrosine, PARP and ICAM-1, and P-selectin expressions. The mechanism of action of PEA/Pol could be related to the sirtuin 1/nuclear factor erythroid 2-related factor 2 (SIRT-1/Nrf2) pathway and nuclear factor (NF)-κB. PEA/Pol administration inhibited NF-κB and increased SIRT-1/Nrf2 expressions. Our results show that PEA/Pol is capable of decreasing inflammatory bowel disease (IBD) DNBS-induced in mice.

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“…There were no significant differences ( P > 0.05) between the 5-Fu, QUE, and CDNPs groups at the end of the experiments. As mentioned above, the BW and food intake of mice reduced significantly after the induction of mucositis, which indicates that mucositis was successfully induced …”

Section: Resultsmentioning

confidence: 99%

“…Tissue samples from the jejunum and distal parts of the colon were processed as reported elsewhere and evaluated under a light microscope . The samples were scored in a blinded manner using a validated scoring system according to the method by Wei et al in 2020 . Histopathological images were taken and digitized using an Olympus BX41 microscope by visualizing at 100× magnification.…”

Section: Experimental Sectionmentioning

confidence: 99%

“…A Takara PrimeScript RT reagent kit was used for reverse transcription into cDNA. The quantitative polymerase chain reaction (PCR) was performed with a Bio-Rad CFX96 real-time PCR detection system, and the reaction conditions were as previously described . Primer sets of the target genes ( IL1-β , IL-6 , IL-10 , NFκB , HIF-1α , and claudin-1 ) and actin gene ( β-Actin ) for each mRNA detected are presented in Table S1 (Supporting Information).…”

Section: Experimental Sectionmentioning

confidence: 99%

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Oral Delivery of Chitosan Derivative-Based Nanoparticles Encapsulating Quercetin to Attenuate Intestinal Injury and Regulate Gut Microbiota Dysbiosis in Mucositis Treatment

Yan

Wei

Zheng

et al. 2021

ACS Food Sci. Technol.

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Although the importance of quercetin (QUE) in diets and as food supplements is well-known, the oral utilization of QUE is limited by its instability, poor solubility, and low bioavailability. To overcome these disadvantages, QUE-loaded chitosan derivative-based nanoparticles (QUE-CDNPs) were fabricated, and their ameliorative efficacy against intestinal injury and gut microbiota dysbiosis in chemotherapy-induced mucositis were investigated. The morphology and structural characteristics of the QUE-CDNPs produced showed an average size of about 370.7 nm and a ζ potential of −21.9 mV. It indicated that the QUE encapsulation efficiency in CDNPs reached 71.2%, and the CDNP carrier substantially increased the bioavailability of QUE compared with that of the free QUE in vivo. QUE-CDNP treatment remarkably alleviated the 5-Fu-induced destruction of villus and crypt in the jejunum. In addition, QUE-CDNPs effectively caused an attenuation of the pro-inflammation factors expression and an enhancement of claudin-1 expression in the jejunum, which consequently ameliorated intestinal barrier function. Furthermore, the QUE-CDNP treatment partially reversed the alterations in the intestinal microbiota of mice with 5-Fu-induced mucositis by decreasing the abundance of Bacteroides. These results demonstrate that the use of the QUE-CDNP-delivery system has the potential to improve both the stability and bioavailability and thus the efficacy of quercetin for the treatment of intestinal mucositis.

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Sulforaphane attenuates 5-fluorouracil induced intestinal injury in mice

Cited by 16 publications

References 82 publications

Nutritional combinatorial impact on the gut microbiota and plasma short-chain fatty acids levels in the prevention of mammary cancer in Her2/neu estrogen receptor-negative transgenic mice

Nutritional combinatorial impact on the gut microbiota and plasma short-chain fatty acids levels in the prevention of mammary cancer in Her2/neu estrogen receptor-negative transgenic mice

PEA/Polydatin: Anti-Inflammatory and Antioxidant Approach to Counteract DNBS-Induced Colitis

Oral Delivery of Chitosan Derivative-Based Nanoparticles Encapsulating Quercetin to Attenuate Intestinal Injury and Regulate Gut Microbiota Dysbiosis in Mucositis Treatment

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