Sulfonate-based networks between eukaryotic phytoplankton and heterotrophic bacteria in the surface ocean

Durham, Bryndan P.; Boysen, Angela K.; Carlson, Laura T.; Groussman, Ryan D.; Heal, Katherine R.; Cain, Kelsy R.; Morales, Rhonda; Coesel, Sacha; Morris, Robert M.; Ingalls, A. E.; Armbrust, E. Virginia

doi:10.1038/s41564-019-0507-5

Cited by 116 publications

(143 citation statements)

References 68 publications

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“…Genes involved in DMSP and DHPS transformation play crucial roles in the sulfur cycling of the oceans (Howard et al, 2006;Durham et al, 2019). The key intermediates MTHB and the dysB enzyme were identified in the marine bacteria Labrenzia aggregata LZB033, indicating that DMSP biosynthesis via Met transamination may operate in heterotrophic bacteria .…”

Section: Genomic Insight Into Dmsp and Dhps Transformation In The Oceanmentioning

confidence: 99%

Chemical Diversity and Biochemical Transformation of Biogenic Organic Sulfur in the Ocean

Tang

2020

Front. Mar. Sci.

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Organic sulfur compounds are not only essential for organismal survival but also indispensable for the sulfur cycle. Over the past few decades, dimethylsulfoniopropionate (DMSP) and dimethyl sulfide (DMS) cycling in the upper ocean have been well characterized from the genetic to the ecosystem level. Recent advances in the study of marine sulfonate transformation have indicated that phytoplankton and microbes play key roles in oceanic sulfur and carbon fluxes. This review provides biochemical details of the major sulfur metabolites, and presents an interlinked reaction network with genetic information on the microbial transformation and mineralization of sulfur compounds. This review also discusses future prospects for the discovery and characterization of novel substrates and enzymes involved in organosulfur cycling, as well as for investigations of deep sea and sedimentary organic sulfur.

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Section: Genomic Insight Into Dmsp and Dhps Transformation In The Oceanmentioning

confidence: 99%

Chemical Diversity and Biochemical Transformation of Biogenic Organic Sulfur in the Ocean

Tang

2020

Front. Mar. Sci.

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“…Durham et al 4 attempt to untangle some of these interactions by focusing on C2-and C3-sulfonates (compounds containing a carbon-sulfur bond (R-SO3-)) ( Fig.1). Sulfonates constitute up to 95% of sulfur in terrestrial soil, being key microbial nutrient sources 5 .…”

mentioning

confidence: 99%

A day in the life of marine sulfonates

Williams

Todd

2019

Nat Microbiol

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“…In exchange, bacteria produce essential factors (e.g., vitamins) (Kazamia et al, 2012;Bertrand et al, 2015) to support the growth of phytoplankton (Amin et al, 2012). Cumulatively, phytoplankton-bacteria symbiosis is believed to play an important role in nutrient availability and major biogeochemical cycles (Buchan et al, 2014;Durham et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Quorum Sensing Regulates ‘swim-or-stick’ Lifestyle in the Phycosphere

Cong

Ochsenkühn

Shibl

et al. 2020

Preprint

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Originality-significance statementMotility and biofilm formation are processes regulated by quorum sensing (QS) in bacteria. Both functions are believed to play an important role in interactions between bacteria and phytoplankton. Here, we show that two bacterial symbionts from the microbial community associated with a ubiquitous diatom switch their motile lifestyle to attached cells while an opportunist bacterium from the same community is incapable of attachment, despite possessing the genetic machinery to do so. Further work indicated that the opportunist lacks QS signal synthases while the symbionts produce three QS signals, one of which is mainly responsible for regulating symbiont colonization of the diatom microenvironment. These findings suggest that QS regulates colonization of diatom surfaces and further work on these model systems will inform our understanding of particle aggregation and bacterial attachment to marine snow and how these processes influence the global carbon cycle.SummaryInteractions between phytoplankton and bacteria play major roles in global biogeochemical cycles and oceanic nutrient fluxes. These interactions occur in the microenvironment surrounding phytoplankton cells, known as the phycosphere. Bacteria in the phycosphere use either chemotaxis or attachment to benefit from algal excretions. Both processes are regulated by quorum sensing (QS), a cell-cell signaling mechanism that uses small infochemicals to coordinate bacterial gene expression. However, the role of QS in regulating bacterial attachment in the phycosphere is not clear. Here, we isolated a Sulfitobacter pseudonitzschiae F5 and a Phaeobacter sp. F10 belonging to the marine Roseobacter group and an Alteromonas macleodii F12 belonging to Alteromonadaceae, from the microbial community of the ubiquitous diatom Asterionellopsis glacialis. We show that only the Roseobacter group isolates (diatom symbionts) can attach to diatom transparent exopolymeric particles. Despite all three bacteria possessing genes involved in motility, chemotaxis, and attachment, only S. pseudonitzschiae F5 and Phaeobacter sp. F10 possessed complete QS systems and could synthesize QS signals. Using UHPLC-MS/MS, we identified three QS molecules produced by both bacteria of which only 3-oxo-C16:1-HSL strongly inhibited bacterial motility and stimulated attachment in the phycosphere. These findings suggest that QS signals enable colonization of the phycosphere by algal symbionts.

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Sulfonate-based networks between eukaryotic phytoplankton and heterotrophic bacteria in the surface ocean

Cited by 116 publications

References 68 publications

Chemical Diversity and Biochemical Transformation of Biogenic Organic Sulfur in the Ocean

Chemical Diversity and Biochemical Transformation of Biogenic Organic Sulfur in the Ocean

A day in the life of marine sulfonates

Quorum Sensing Regulates ‘swim-or-stick’ Lifestyle in the Phycosphere

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