Sulfite serving as a pretreatment method for alkaline fermentation to enhance short-chain fatty acid production from waste activated sludge

Liu, Xuran; Du, Mingting; Yang, Jingnan; Wu, Yanxin; Xu, Qiuxiang; Wang, Dongbo; Yang, Qi; Yang, Guojing; Li, Xiaoming

doi:10.1016/j.cej.2019.123991

Cited by 149 publications

(31 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The prepared DNA samples were subjected to Illumina sequencing (using Illumina Hiseq 2500 platform, Guangdong Magigene Biotechnology Co., Ltd. Guangzhou, China). More detailed procedures about Illumina sequencing can be referred to elsewhere (Liu et al, 2020). The sequence data (deposited in the National Center for Biotechnology Information; BioProject ID: PRJNA779218, https://www.ncbi.nlm.nih.gov/bioproject/ PRJNA779218/) were transformed into Raw Reads by fastp software (version 0.14.1) through base recognition and analysis, and then spliced and filtered to get the Clean Tags (using usearch-fastq mergepairs V10 and fastp version 0.14.1).…”

Section: Illumina Sequencing and Data Analysismentioning

confidence: 99%

Effect of Thermal Hydrolysis Pretreatment on Anaerobic Digestion of Protein-Rich Biowaste: Process Performance and Microbial Community Structures Shift

Shi

Zhang

et al. 2022

Front. Environ. Sci.

View full text Add to dashboard Cite

To reveal the effects of thermal hydrolysis (TH) pretreatment (THPT) on anaerobic digestion (AD) of protein-rich substrates, discarded tofu was chosen as the object, and its batch AD tests of tofu before and after being subjected to TH at gradually increasing organic loads were carried out and the AD process characteristics were compared; furthermore, its continuous AD tests without and with THPT were also conducted and the difference of the microbial community structures was investigated. The results showed that, during AD of protein-rich tofu with increase in the organic load, inhibition from severe acidification prior to accumulation of ammonia nitrogen (AN) occurred. THPT helped overcome the acidification inhibition present in batch AD of tofu at such a high TS content of 3.6%, to obtain the maximum methane yield rate of 589.39 ml·(gVS)−1. Continuous AD of protein-rich tofu heavily depended on ammonia-tolerant hydrogenotrophic methanogens and bacteria. The continuous AD processes acclimated by HT substrates seemed to be resistant to severe organic loads, by boosting growth of ammonia-tolerant microorganisms, above all methylotrophic methanogens such as the genera RumEnM2 and methanomassiliicoccus. The process response of continuous AD of HT tofu was hysteretic, suggesting that a sufficiently long adaptation period was required for stabilizing the AD system.

show abstract

Section: Illumina Sequencing and Data Analysismentioning

confidence: 99%

Effect of Thermal Hydrolysis Pretreatment on Anaerobic Digestion of Protein-Rich Biowaste: Process Performance and Microbial Community Structures Shift

Shi

Zhang

et al. 2022

Front. Environ. Sci.

View full text Add to dashboard Cite

show abstract

“…Reported process duration for maximal VFA concentration is 5-8 days [21,29,30]. The concentration of VFA increases rapidly during the first 4-8 days thereafter reaching a plateau then maintaining the concentration [31,32]. Zhao et al show that the production of short-chained fatty acids from waste activated sludge peaks at day 6-10 depending on pH [33].…”

Section: Introductionmentioning

confidence: 99%

“…Alkaline conditions can be achieved by the addition of NaOH, Ca(OH) 2 , KOH, and NH 4 OH [39]. Previously published studies on alkaline fermentation focused on production of fatty acids from municipal wastewater treatment, waste activated sludge [32,40], and wetland plant litter [41]. The aspects that have been investigated are as follows: addition of sulfite [32]; characterization of dissolved organic matter [40]; the impact of ammonia and free ammonia [33]; addition of bio-surfactants [42]; and the contribution of biotic and abiotic factors at different pH [41].…”

Section: Introductionmentioning

confidence: 99%

The effect of pH, temperature, and inoculum on the fermentation of pulp and paper biosludge: increasing the nutrient availability for rearing of black soldier fly larvae

Niero

Norgren

Kumpienė

et al. 2022

Biomass Conv. Bioref.

View full text Add to dashboard Cite

Pulp and paper production is one of the largest global industries producing annually 400 million metric tons of pulp and paper products and 6 million tons of pulp and paper biosludge (PPBS). From a resource efficiency and sustainability perspective, there is a need for improving PPBS management. This study assessed fermentation of PPBS as pretreatment to improve PPBS feasibility as feed for black soldier fly larvae. The impact of temperature, pH, and inoculum on the concentration of soluble chemical oxygen demand (sCOD) and volatile fatty acids (VFA) was assessed. An initial pH of 10 and the addition of inoculum from an anaerobic digester substantially increased the concentration of sCOD. The obtained concentration of VFA was low compared to the VFA concentration needed to improve the growth of Black Soldier Fly Larvae (BSFL). The PPBS is recalcitrant to fermentation because of the high content of lignocellulose. Fermentation as done in this study does not convert PPBS to a feasible feed for black soldier fly larvae; thus, further research on improved fermentation is needed. However, fermentation at alkaline pH and addition of inoculum do increase the final pH of PPBS which improves its feasibility as feed for BSFL. Future studies should explore pH > 10 and temperatures > 55 °C to increase sCOD and improving generation of VFA by removal of inhibiting substances, testing other types of inoculum (rumen microorganisms) and co-fermentation.

show abstract

“…A number of waste sludge pretreatment processes have been used to increase sludge digestion rates and/or the amount of solids reduction in anaerobic digestion, but there have been limited investigations on the effect of pretreatment methods on digester foaming. Among newer methods, freezing activated nitrite‐based pretreatment has been shown to be effective in improving the efficiency of anaerobic digestion of WAS (Liu et al ., 2020a). In addition, alkaline fermentation using sulphite has been shown to enhance the production of short‐chain fatty acids and aid in sludge settling (Liu et al ., 2020b).…”

Section: Introductionmentioning

confidence: 99%

Effect of waste activated sludge pretreatment methods to mitigate Gordonia foaming potential in anaerobic digestion

Garg

Stensel

Bucher

et al. 2020

Water & Environment J

View full text Add to dashboard Cite

In this study, waste activated sludge obtained from two full‐scale treatment plants with foaming issues was pretreated with acid/alkali treatment, acid‐phase fermentation, thermal treatment, ultrasonic treatment and metal salt treatment to investigate their effect on foam mitigation. Waste activated sludge was characterized for foaming index prior and after each pretreatment method. Among all pretreatment methods investigated, acid/alkali treatment and acid phase fermentation showed highest reduction of 53% in foaming and in inactivation of Gordonia amarae filaments. Pretreatment methods that resulted in sharp decrease in the foaming index concurred with higher amounts of dead foam formers as confirmed using live/dead staining and the PMA‐qPCR technique. Pretreating with iron(III)chloride gave good foaming reductions with 54% decrease in foaming potential at a concentration of 260 mg/L but did not result in a decline of foam formers as confirmed by live/dead staining. Ultrasonic treatment did not prove effective in lowering the foaming index or killing the G. amarae filaments.

show abstract

Sulfite serving as a pretreatment method for alkaline fermentation to enhance short-chain fatty acid production from waste activated sludge

Cited by 149 publications

References 42 publications

Effect of Thermal Hydrolysis Pretreatment on Anaerobic Digestion of Protein-Rich Biowaste: Process Performance and Microbial Community Structures Shift

Effect of Thermal Hydrolysis Pretreatment on Anaerobic Digestion of Protein-Rich Biowaste: Process Performance and Microbial Community Structures Shift

The effect of pH, temperature, and inoculum on the fermentation of pulp and paper biosludge: increasing the nutrient availability for rearing of black soldier fly larvae

Effect of waste activated sludge pretreatment methods to mitigate Gordonia foaming potential in anaerobic digestion

Contact Info

Product

Resources

About