Sulfide restrains the growth of Methylocapsa acidiphila converting renewable biogas to single cell protein

Xu, Mingyi; Zhou, Huihui; Yang, Xiaoyang; Angelidaki, Irini; Zhang, Yifeng

doi:10.1016/j.watres.2020.116138

Cited by 44 publications

(28 citation statements)

References 59 publications

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“…Maximum production of Rhizopus oligosporus mycelium with higher protein content was achieved at 7.5% (v/v) inoculum size (Mahat and MacRae, 1992). A higher optimum inoculum level (20%) was reported by Xu et al (2020) in acid tolerance Methylocapsa acidiphila. In addition to protein yield, inoculum size significantly affects microbial enzyme synthesis during solid-state fermentation (Kosseva, 2013).…”

Section: Inoculation and Incubationmentioning

confidence: 88%

“…Liquid substrates reported are cheese whey (Yadav et al, 2015;Monkoondee et al, 2016), non-dairy creamer wastewater (Dewi et al, 2017), sugar refinery wastewater (Saejung and Salasook, 2020), pineapple peel juice, and rice washing water (Mujdalipah and Putri, 2020), latex rubber sheet wastewater (Kornochalert et al, 2014), dephenolized olive mill wastewater (Giavasis and Petrotos, 2016), sugarcane molasses (Hansen and Cheong, 2019;Spalvins et al, 2018a), soybean molasses (Gao et al, 2012), deproteinized leaf juice (Chanda and Chakrabarti, 1996;Spalvins et al, 2018a), food waste-derived volatile fatty acids (Wainaina et al, 2020), waste milk (Myint et al, 2020), municipal wastewater treatment effluent (Steinberg et al, 2017), food processing wastewater (Xu et al, 2020). Urban bio-waste is also a potential substrate for SCP production after anaerobic digestion; methane produce during digestion can serve as a carbon source in the production of SCP rich in essential amino acids (Khoshnevisan et al, 2019).…”

Section: Food Wastes As Substrates For Production Of Scpmentioning

confidence: 99%

“…pH adjustment using acidic effluent was the only preparatory operation reported by Monkoondee et al (2016) in cheese whey. Xu et al (2020) combine sterilization and pH adjustment in acid wastewater from food processing. Similar pre-treatment was also reported by Saejung and Salasook (2020) in the production of biomass using sugarcane processing wastewater.…”

Section: Media Preparation Using Liquid Wastementioning

confidence: 99%

“…Inoculum levels influence the growth of microorganisms (Koutsoumanis and Sofos, 2005;Xu et al, 2020) by affecting reference time to detection (Bidlas et al, 2008), lag time (Robinson et al, 2001), and microbial enzymes production (Dinarvand et al, 2017;Ilgın et al, 2020). The lower the inoculum level the higher the pH and aw levels for optimum growth (Skandamis et al, 2007).…”

Section: Inoculation and Incubationmentioning

confidence: 99%

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Review on Production of Single-Cell Protein from Food Wastes

Abdullahi

Dandago

Yunusa

2021

Turkish JAF Sci.Tech.

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The roles of protein in bodybuilding and the regulation of biological processes are important in sustaining life. A large amount of protein is required by both humans and animals and this cannot be supplied by only conventional sources. This is because of the rapid increase in world population. The present sources of protein will not meet global protein demand in years to come. Scientists explore the production of single-cell protein (SCP), as an alternative source of protein, through the utilization of wastes and low-value materials. SCP can supply high-quality protein containing both essential and non-essential amino acids that can be utilized by humans and animals. Protein from microbial biomass is cheaper than animal proteins because the substrates used in the production are generally cheaper and more readily available. Moreover, the production process does not require arable land and the entire process can be completed within a short time. This article reviewed the process of SCP production. Different raw materials used in the production and variations in growth media preparation methods were discussed. Various sources of fermentation microorganisms and their potential substrate were reviewed. Growth media enrichment using different carbon, nitrogen, and mineral sources was also discussed.

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Section: Inoculation and Incubationmentioning

confidence: 88%

Section: Food Wastes As Substrates For Production Of Scpmentioning

confidence: 99%

Section: Media Preparation Using Liquid Wastementioning

confidence: 99%

Section: Inoculation and Incubationmentioning

confidence: 99%

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Review on Production of Single-Cell Protein from Food Wastes

Abdullahi

Dandago

Yunusa

2021

Turkish JAF Sci.Tech.

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“…A carefully selected synthetic coculture, composed of community members with complementary characteristics could enable higher adaptability to these potentially variable inputs and therefore contribute to overall system stability (Bothe et al, 2002). Furthermore, it was shown that the presence of H 2 S in the biogas affects the growth and nutritional quality of Methylocapsa acidiphila (Xu et al, 2020). The composition of the cultivation medium is also important, as it has been reported that the addition of 100 μg Cu 2+ /L to NMS medium significantly affects the cell density of MOB since Cu is a key element for the methanotrophic metabolism (Semrau et al, 2018).…”

Section: Reality Check: Current Knowledge Gaps and Limitationsmentioning

confidence: 99%

From Biogas and Hydrogen to Microbial Protein Through Co-Cultivation of Methane and Hydrogen Oxidizing Bacteria

Kerckhof

Sakarika

Giel

et al. 2021

Front. Bioeng. Biotechnol.

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Increasing efforts are directed towards the development of sustainable alternative protein sources among which microbial protein (MP) is one of the most promising. Especially when waste streams are used as substrates, the case for MP could become environmentally favorable. The risks of using organic waste streams for MP production–the presence of pathogens or toxicants–can be mitigated by their anaerobic digestion and subsequent aerobic assimilation of the (filter-sterilized) biogas. Even though methane and hydrogen oxidizing bacteria (MOB and HOB) have been intensively studied for MP production, the potential benefits of their co-cultivation remain elusive. Here, we isolated a diverse group of novel HOB (that were capable of autotrophic metabolism), and co-cultured them with a defined set of MOB, which could be grown on a mixture of biogas and H2/O2. The combination of MOB and HOB, apart from the CH4 and CO2 contained in biogas, can also enable the valorization of the CO2 that results from the oxidation of methane by the MOB. Different MOB and HOB combinations were grown in serum vials to identify the best-performing ones. We observed synergistic effects on growth for several combinations, and in all combinations a co-culture consisting out of both HOB and MOB could be maintained during five days of cultivation. Relative to the axenic growth, five out of the ten co-cultures exhibited 1.1–3.8 times higher protein concentration and two combinations presented 2.4–6.1 times higher essential amino acid content. The MP produced in this study generally contained lower amounts of the essential amino acids histidine, lysine and threonine, compared to tofu and fishmeal. The most promising combination in terms of protein concentration and essential amino acid profile was Methyloparacoccus murrelli LMG 27482 with Cupriavidus necator LMG 1201. Microbial protein from M. murrelli and C. necator requires 27–67% less quantity than chicken, whole egg and tofu, while it only requires 15% more quantity than the amino acid-dense soybean to cover the needs of an average adult. In conclusion, while limitations still exist, the co-cultivation of MOB and HOB creates an alternative route for MP production leveraging safe and sustainably-produced gaseous substrates.

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