Sulfhydryl oxidation induces rapid calcium release from sarcoplasmic reticulum vesicles.

Trimm, Jonathan L.; Salama, Guy; Abramson, Jonathan J.

doi:10.1016/s0021-9258(18)66682-7

Cited by 162 publications

(4 citation statements)

References 38 publications

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“…Low power laser irradiation has also been reported to cause accelerated Ca 2ϩ uptake by bull sperm cells, 13 mouse spermatozoa 14 and human lymphocytes 15 and isolated mitochondria. 20, 21 The release of calcium from intracellular stores after low power laser irradiation is consistent with the previous finding of ROS generation, in that even millimolar concentrations of H 2 O 2 were required to stimulate Ca 2ϩ release from SR vesicles, as was shown by Trimm et al 34 Calcium elevation has also been reported to play an important role in cell rescue during photodynamic induction of ROS at the single cell level. 35,36 In addition, Ca 2ϩ has been proposed to act as a mediator of p70 activation by ROS.…”

Section: Discussionsupporting

confidence: 87%

Human fibroblast alterations induced by low power laser irradiation at the single cell level using confocal microscopy

Alexandratou

Yova

Handris

et al. 2002

Photochem Photobiol Sci

153

112

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Low power laser irradiation is regarded to have a significant role in triggering cellular proliferation and in treating diseases of diverse etiologies. The present work contributes to the understanding of the mechanisms of action by studying low power laser effects in human fibroblasts. Confocal laser scanning microscopy is used for irradiation and observation of the same area of interest allowing the imaging of laser effects at the single cell level and in real time. Coverslip cultures were placed in a small incubation chamber for in vivo microscopic observation. Laser stimulation of the cells was performed using the 647 nm line of the confocal laser through the objective lens of the microscope. Mitochondrial membrane potential (delta psi(m)), intracellular pH, calcium alterations and generation of reactive oxygen species (ROS) were monitored using specific fluorescent vital probes. The induced effects were quantified using digital image processing techniques. After laser irradiation, a gradual alkalinization of the cytosolic pH and an increase in mitochondrial membrane potential were observed. Recurrent spikes of intracellular calcium concentration were also triggered by laser. Reactive oxygen species were generated as a result of biostimulation. No such effects were monitored in microscopic fields other than the irradiated ones.

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Section: Discussionsupporting

confidence: 87%

Human fibroblast alterations induced by low power laser irradiation at the single cell level using confocal microscopy

Alexandratou

Yova

Handris

et al. 2002

Photochem Photobiol Sci

153

112

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“…Thus SH reagents (Abramson et al, 1995), free radicals (Stoyanovsky et al, 1994), and hydrogen peroxide (Favero et al, 1995) activate RyR channels incorporated into planar lipid bilayers. Heavy metals (Abramson et al, 1983;Trimm et al, 1986;Salama et al, 1992), mercurials (Bindoli and Fleischer, 1983), dithiodipyridines (Nagura et al, 1988;Prabhu and Salama, 1990;Donoso et al, 1997), and derivatives of nitric oxide (Stoyanovsky et al, 1997) trigger calcium release from isolated SR vesicles as well. Furthermore, SH reagents shift the calcium activation curve of ryanodine binding to the left (Stuart et al, 1992;Favero et al, 1995), and heavy metals enhance the calcium sensitivity of tension development in skinned fibers from rabbit psoas muscle (Salama et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Sulfhydryl Oxidation Modifies the Calcium Dependence of Ryanodine-Sensitive Calcium Channels of Excitable Cells

Marengo

Hidalgo

Bull

1998

Biophysical Journal

196

155

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The calcium dependence of ryanodine-sensitive single calcium channels was studied after fusing with planar lipid bilayers sarcoendoplasmic reticulum vesicles isolated from excitable tissues. Native channels from mammalian or amphibian skeletal muscle displayed three different calcium dependencies, cardiac (C), mammalian skeletal (MS), and low fractional open times (low Po), as reported for channels from brain cortex. Native channels from cardiac muscle presented only the MS and C dependencies. Channels with the MS or low Po behaviors showed bell-shaped calcium dependencies, but the latter had fractional open times of <0.1 at all [Ca2+]. Channels with C calcium dependence were activated by [Ca2+] < 10 microM and were not inhibited by increasing cis [Ca2+] up to 0.5 mM. After oxidation with 2,2'-dithiodipyridine or thimerosal, channels with low Po or MS dependencies increased their activity. These channels modified their calcium dependencies sequentially, from low Po to MS and C, or from MS to C. Reduction with glutathione of channels with C dependence (native or oxidized) decreased their fractional open times in 0.5 mM cis [Ca2+], from near unity to 0.1-0.3. These results show that all native channels displayed at least two calcium dependencies regardless of their origin, and that these changed after treatment with redox reagents.

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“…– in unstimulated monocyte/macrophage [ 33 ] and in isolated mitochondria from guinea-pig cardiac myocytes [ 34 ]. Oxidation of the thiol groups in RyR2 by ROS enhances its channel activity, whereas their reduction inhibits the channels [ 35 - 37 ]. Therefore, in the next series of experiments, we tested whether DPI also affects mitochondrial ROS level in rat ventricular myocytes under control conditions using confocal imaging with MitoSOX-red, the mitochondrial O 2 .…”

Section: Resultsmentioning

confidence: 99%

“…Nevertheless, reversible and quick effects of DPI on the spark frequency ( Fig. 4 ) may reflect other mechanism involved in modulation of RyRs by DPI, such as reduction of thiol groups on RyR by decrease of mitochondrial ROS level in the vicinity [ 11 , 35 - 37 ]. Since we observed mitochondrial ROS reduction during application of DPI at similar concentrations of DPI ( Fig.…”

Section: Discussionmentioning

confidence: 99%

The NADPH oxidase inhibitor diphenyleneiodonium suppresses Ca²⁺ signaling and contraction in rat cardiac myocytes

Le,

Trinh,

Luong

et al. 2024

Korean J Physiol Pharmacol

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Sulfhydryl oxidation induces rapid calcium release from sarcoplasmic reticulum vesicles.

Cited by 162 publications

References 38 publications

Human fibroblast alterations induced by low power laser irradiation at the single cell level using confocal microscopy

Human fibroblast alterations induced by low power laser irradiation at the single cell level using confocal microscopy

Sulfhydryl Oxidation Modifies the Calcium Dependence of Ryanodine-Sensitive Calcium Channels of Excitable Cells

The NADPH oxidase inhibitor diphenyleneiodonium suppresses Ca²⁺ signaling and contraction in rat cardiac myocytes

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Sulfhydryl oxidation induces rapid calcium release from sarcoplasmic reticulum vesicles.

Cited by 162 publications

References 38 publications

Human fibroblast alterations induced by low power laser irradiation at the single cell level using confocal microscopy

Human fibroblast alterations induced by low power laser irradiation at the single cell level using confocal microscopy

Sulfhydryl Oxidation Modifies the Calcium Dependence of Ryanodine-Sensitive Calcium Channels of Excitable Cells

The NADPH oxidase inhibitor diphenyleneiodonium suppresses Ca2+ signaling and contraction in rat cardiac myocytes

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The NADPH oxidase inhibitor diphenyleneiodonium suppresses Ca²⁺ signaling and contraction in rat cardiac myocytes