Sulfation of the extracellular polysaccharide produced by the edible mushroom Pleurotus sajor-caju alters its antioxidant, anticoagulant and antiproliferative properties in vitro

Telles, Cinthia Beatrice Silva; Sabry, Diego Araújo; Almeida-Lima, Jailma; Costa, Mariana Santana Santos Pereira; Melo-Silveira, Raniere Fagundes; Trindade, Edvaldo S.; Sassaki, Guilherme L.; Wisbeck, Elisabeth; Furlan, Sandra Aparecida; Leite, Edda Lisboa; Rocha, Hugo Alexandre Oliveira

doi:10.1016/j.carbpol.2011.02.038

Cited by 51 publications

(31 citation statements)

References 45 publications

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“…In contrast, other studies have shown increased anticoagulant activity in purified fractions (Pawlaczyk et al, 2009). With respect to PT test it was observed no inhibitory activity of TPL, demonstrating no interference in the extrinsic coagulation pathway, similarly to sulfated polysaccharides such as heparin (Telles et al, 2011;Weirong Cai, Xie, Chen, & Zhang, 2013). Thus, based in the anticoagulant activity demonstrated by TPL and FIII in the aPTT test, it is possible to suggest that hexuronic acid residues contribute to the action of the polysaccharides of G. spinosa barks in the intrinsic coagulation pathway.…”

Section: Effect Of G Spinosa Polysaccharides In Coagulationmentioning

confidence: 76%

Purified polysaccharides of Geoffroea spinosa barks have anticoagulant and antithrombotic activities devoid of hemorrhagic risks

Souza

Assreuy

Madeira

et al. 2015

Carbohydrate Polymers

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Polysaccharides were extracted from the barks of Geoffroea spinosa, purified using anion exchange chromatography and characterized by chemical and methylation analysis, complemented by infrared and NMR spectroscopies. These polysaccharides were tested for their anticoagulant, antithrombotic and antiplatelet activities and also for their effects on bleeding. Unfractionated polysaccharide contains low levels of protein and high levels of carbohydrate (including hexuronic acid). The purified polysaccharides (fractions FII and FIII) are composed of arabinose (Ara), rhamnose (Rha), hexuronic acid, small amounts of galactose, but no sulfate ester. They have highly complex structure, which was partially characterized. NMR and methylation analysis indicate that the polysaccharides have a core of α-Rhap and branches of 5-linked α-Araf. Residues of 4-linked α-GalpA are also found in the structure. The unfractionated (TPL) and fraction FIII, but not fractions FI and FII, prolonged the activated partial thromboplastin time (aPTT). TPL, FII and FIII inhibited the platelet aggregation induced by ADP. More significantly, both unfractionated and purified fractions exhibited potent antithrombotic effect (31-60%) and the fractions did not modify the bleeding tendency. These plant polysaccharides could be alternative source of new anticoagulant, antiplatelet and antithrombotic compounds devoid of the undesirable risk of hemorrhage.

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Section: Effect Of G Spinosa Polysaccharides In Coagulationmentioning

confidence: 76%

Purified polysaccharides of Geoffroea spinosa barks have anticoagulant and antithrombotic activities devoid of hemorrhagic risks

Souza

Assreuy

Madeira

et al. 2015

Carbohydrate Polymers

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“…It has been reported that addition of electron-withdrawing groups to the polysaccharide enhanced antioxidant activity (Telles et al, 2011). Thus, the presence of a sulfate group could increase the radical scavenging activity.…”

Section: B-carotene-linoleic Acid Assaymentioning

confidence: 99%

“…(Wang, Wang et al, 2010). It has been reported that the sulfated polysaccharides exerted better biological activities in comparison with non-sulfated polysaccharides, such as anti-coagulant, antiviral, immunostimulant, hypoglycemic, anti-oxidant and antitumour activities (Costa et al, 2010;Telles et al, 2011;Wijesekara, Pangestuti, & Kim, 2011). Therefore, sulfated modification could be considered as an effective way to enhance the biological activities of polysaccharides.…”

Section: Introductionmentioning

confidence: 96%

Sulfated modification of the polysaccharides from Ganoderma atrum and their antioxidant and immunomodulating activities

et al. 2015

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“…Carboxymethylation of polysaccharides from Tremella fuciformis could effectively enhance their antioxidant and moisture‐preserving activities (Wang, Zhang, & Zhao, ). The extracellular polysaccharides from Pleurotus sajor‐caju did not show anticoagulant or antiproliferative activities, but the sulfated derivatives demonstrated promising antioxidant, antiproliferative, and anticoagulant properties (Telles et al., ). Our previous study reported that the carboxymethylation of polysaccharides extracted from Morchella angusticepes Peck (PMEP) was an efficient way to improve the cholesterol‐lowering activity in vivo (Li et al., ).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Antiproliferative Activities of Modified Polysaccharides Originally Isolated from Morchella Angusticepes Peck

Tang

Chen

et al. 2019

Journal of Food Science

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Polysaccharides extracted from Morchella angusticepes Peck (PMEP) were chemically modified to obtain sulfated, carboxymethylated, and acetyled derivatives. Results showed that the acetyled derivatives with the degree of substitution (0.40 ± 0.07) exerted higher antioxidant ability than native polysaccharides, demonstrating inhibitory effects on growth of human hepatoma cells (EC50 = 0.710 ± 0.002 mg/mL) and human colon cancer cells (EC50 = 1.229 ± 0.008 mg/mL). It indicated that the acetylation was a favorable way to enhance the bioactivities of PMEP. Practical Application Polysaccharides extracted from Morchella angusticepes Peck (PMEP) have many health‐promoting properties. Chemical modifications could improve the bioactivities of polysaccharides. We demonstrated that acetylation enhanced the cellular antioxidant and antiproliferative activities of PMEP. The results support further research that explores clinical utility, and may justify ex vivo and in vivo designs toward that end.

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Sulfation of the extracellular polysaccharide produced by the edible mushroom Pleurotus sajor-caju alters its antioxidant, anticoagulant and antiproliferative properties in vitro

Cited by 51 publications

References 45 publications

Purified polysaccharides of Geoffroea spinosa barks have anticoagulant and antithrombotic activities devoid of hemorrhagic risks

Purified polysaccharides of Geoffroea spinosa barks have anticoagulant and antithrombotic activities devoid of hemorrhagic risks

Sulfated modification of the polysaccharides from Ganoderma atrum and their antioxidant and immunomodulating activities

Antioxidant and Antiproliferative Activities of Modified Polysaccharides Originally Isolated from Morchella Angusticepes Peck

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