Sulfation in isolated enterocytes of guinea pig: Dependence on inorganic sulfate

Schwarz, Leonard; Schwenk, Michael

doi:10.1016/0006-2952(84)90105-9

Cited by 21 publications

(5 citation statements)

References 20 publications

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“…mrp2, an important member of the ABC family of transporters (20), is a candidate for pumping conjugated compounds out of the intestinal cells. mrp2 is principally expressed in the proximal small intestine and follows a pattern of distribution similar to that of conjugating enzymes (10,25,33,38,41). Regulation of mrp2 expression during perinatal events may determine the fate and, consequently, the toxicity of xenobiotics introduced into the maternal intestine.…”

Section: Discussionmentioning

confidence: 99%

“…Thus mrp2, or canalicular multispecific organic anion transporter, mediates the ATP-dependent transport of glucuronide, sulfate, and glutathione conjugates across the apical domain not only of hepatocytes and renal tubular cells (22) but also of enterocytes. mrp2 protein is preferentially localized in the proximal intestine and gradually decreases from the jejunum to the distal ileum (33), following a pattern of distribution similar to that of the conjugating enzymes in the rat (10,25,38,41). In addition, mrp2 expression is highest at the tip region of the intestinal villus.…”

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confidence: 99%

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Expression of multidrug resistance-associated protein 2 in small intestine from pregnant and postpartum rats

Mottino

Hoffman

Jennes

et al. 2001

American Journal of Physiology-Gastrointestinal and Liver Physi

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We analyzed the expression of multidrug resistance-associated protein 2 (mrp2) in the small intestine of control female rats and in rats during late pregnancy (19-20 days of pregnancy) and lactation (2-4, 10-14, and 21 days after delivery). Western blot analysis was performed on brush-border membranes prepared from different regions of the small intestine. Expression of mrp2 was maximal in the proximal segments for all experimental groups, was preserved in pregnant rats, and increased by 100% in postpartum rats by late lactation with respect to control animals. Northern blot analysis of mrp2 mRNA revealed a positive correlation with protein levels. Transport of S-glutathione-dinitrophenol (DNP-SG) from the intestinal cell to the lumen was analyzed in the everted intestinal sac model. Secretion of DNP-SG was not altered in pregnant rats but increased in lactating animals by late lactation. Intestinal mrp2 mRNA, protein, and transport activity are increased in lactating rats, suggesting that this may represent an adaptive mechanism to minimize the toxicity of dietary xenobiotics in response to increased postpartum food consumption.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Expression of multidrug resistance-associated protein 2 in small intestine from pregnant and postpartum rats

Mottino

Hoffman

Jennes

et al. 2001

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…While the incidence of cancer in the human small intestine is only 0.1-0.3% of all malignant neoplasms, that in the colon is 15%, and is the second most prevalent cancer in the United States (American Cancer Society 1989). In contrast, the small intestine has 3 times greater glucuronosyl transferase (Hanninen et al 1968), 30 times greater glutathione-S-transferase activity (Siegers et al 1988), and significantly higher sulphotransferase activity (Schwartz and Schwenk 1984;Hanninen et al 1987;Cappiello et al 1990). Moreover, the isoenzyme composition of each of three enzyme systems, glucuronosyltransferase, glutathione-S-transferase and cytochrome P-450 was greater in the small intestine than in the colon (Peters et al 199 1).…”

Section: Figsmentioning

confidence: 98%

Microbial succession and intestinal enzyme activities in the developing rat

Chang

Chadwick

Allison

et al. 1994

Journal of Applied Bacteriology

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The succession of gut bacteria and selected intestinal enzyme activities in developing 7-35-d-old rats was studied. Aerobes and anaerobes were identified as members of four broad major bacterial groups, i.e. Gram-positive rods, Gram-positive cocci, Gram-negative rods and obligate anaerobes. The enzyme activities of nitro and azo reductases, beta-glucuronidase, dechlorinase and dehydrochlorinase were determined by anaerobic incubation of intestinal homogenates with 3,4-dichloronitrobenzene, methyl orange, p-nitrophenyl-beta-D-glucuronide, and p,p'-DDT respectively. Nitroreductase and azo reductase activities increased significantly with the appearance of anaerobes in the large intestine. No increase in either nitroreductase or azo reductase activities in the small intestine was found. The early and high level of beta-glucuronidase activity in the small and large intestines coincided with high numbers of coliforms recovered in 7 and 14 d animals. Dehydrochlorinase activity appeared early but was undetectable at both 21 and 28 d. Its activity increased at 35 d. Dechlorinase activity was variable in development. The rapid changes in the microbial flora and intestinal enzyme activities may influence the susceptibility of pre-pubescent rats to a variety of toxicants. Therefore, age-dependent toxicity may be important in the risk assessment of some environmental chemicals.

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“…at ASPET Journals on May 7, 2018 dmd.aspetjournals.org intestinal wall, from duodenum to ileum (Clifton and Kaplowitz, 1977;Pinkus et al, 1977;Schwarz and Schwenk, 1984;Dubey and Singh, 1988a). The human intestinal CYP3A4 shows a slightly lower level at the duodenum before levels rise again at the jejunum, then finally decreasing toward the ileum .…”

Section: Intestinal Drug Transport and Metabolismmentioning

confidence: 99%

Modeling of Intestinal Drug Absorption: Roles of Transporters and Metabolic Enzymes (For the Gillette Review Series)

2003

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Sulfation in isolated enterocytes of guinea pig: Dependence on inorganic sulfate

Cited by 21 publications

References 20 publications

Expression of multidrug resistance-associated protein 2 in small intestine from pregnant and postpartum rats

Expression of multidrug resistance-associated protein 2 in small intestine from pregnant and postpartum rats

Microbial succession and intestinal enzyme activities in the developing rat

Modeling of Intestinal Drug Absorption: Roles of Transporters and Metabolic Enzymes (For the Gillette Review Series)

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