Sulfated vizantin suppresses mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen that causes severe infections, such as pneumonia and bacteremia. Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection. In this study, we determined whether sulfated vizantin affects P. aeruginosa flagellar motility in the absence of direct antimicrobial activity. We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity. To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ. These results indicated that sulfated vizantin is an effective inhibitor of flagellar motility in P. aeruginosa.

Design and Synthesis of Aryl Amide Derivatives Containing Thiazole as Type III Secretion System Inhibitors against Pseudomonas aeruginosa

“…aeruginosa, playing a role in the pathogenicity of certain mucosal infections and are essential to the virulence of the bacteria. Studies have indicated a high degree of homology between genes encoding the T3SS and those involved in flagella production . Therefore, as we observed that compound II-22 could suppress the expression of T3SS-related genes in P.…”

“…Studies have indicated a high degree of homology between genes encoding the T3SS and those involved in flagella production. 25 Therefore, as we observed that compound II-22 could suppress the expression of T3SS-related genes in P. aeruginosa, we proceeded to investigate the impact of compound II-22 on the motility of P. aeruginosa. We tested the motility of P. aeruginosa with or without the addition of compound II-22.…”

Development of a Water Soluble Self-assembling Analogue of Vizantin

“…Finally, after removing the ion-exchange resin, the mixture was subjected to SEC with Sephadex ® G-25 to give the desired 3. [26][27][28][29][30][31] However, the overall yield of 3 from vizantin was only 29%. Thus, we embarked on improving the synthesis method in more detail (Table 1).…”

Structure and functional analysis of the Legionella pneumophila chitinase ChiA reveals a novel mechanism of metal-dependent mucin degradation

“…Bacteria were then diluted in BYE broth to OD 660 of 0.3. Transwell plates (Corning) were used for analysis of bacterial crossing of a mucin layer following a previously described protocol [80,81]. 500 μl of sterile BYE broth was added to the bottom of either empty wells, or wells containing 3.0 μm transwells.…”