Sulfated oligosaccharides block antibodies to many Dictyostelium discoideum acid hydrolases.

Freeze, Hudson H.; Mierendorf, Robert C.; Wunderlich, R; Dimond, Randall L.

doi:10.1016/s0021-9258(18)91010-0

Cited by 42 publications

(3 citation statements)

References 18 publications

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“…We have also demonstrated that these sulfate groups elicit specific immune responses and are involved in heart damage (Acosta et al, 2008). Our results in conjunction with those reported in Dictyostelium discoydeum are, as far as we know, the only ones describing the atypical antigenic properties of this type of structures (Freeze et al, 1984;Acosta et al, 2008). Furthermore, in T. cruzi, sulfated structures have been described as part of glycolipids such as sulfoglycosphingolipids (or sulfatides) (Uhrig et al, 1992;Acosta et al, 2012).…”

Section: Introductionsupporting

confidence: 81%

“…Sulfation is a post-translational modification which is critical for many biological events such as ligand-receptor recognition, receptor-mediated signaling, cellular adhesion and structural maintenance (Kovensky, 2009;Fukuda et al, 2001;Rapraeger et al, 1991). In T. cruzi, sulfated structures have been identified as part of glycolipids (Freeze et al, 1984) and glycoproteins (Barboza et al, 2005), however nothing is known about the metabolic pathways involved in their synthesis. In previous works, we have determined the chemical composition of sulfatides (Acosta et al, 2012) and reported for the first time the presence of a sulfated glycoprotein when we described the N-glycans present in the C-T domain of the Cz (Barboza et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

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Effects of chlorate on the sulfation process of Trypanosoma cruzi glycoconjugates. Implication of parasite sulfates in cellular invasion

et al. 2014

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Section: Introductionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Effects of chlorate on the sulfation process of Trypanosoma cruzi glycoconjugates. Implication of parasite sulfates in cellular invasion

et al. 2014

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“…Noticeably, in T. cruzi , we have shown the antigenic properties of these structures in glycoconjugates and cross-reactivity between sulfatides and Cz was determined ( Kovensky, 2009 ; Acosta et al., 2012 ). Until the discovery of the trypanosomatid sulfotope antigenic properties, the only sulfated HM-type glycans with similar properties described have been found in components of the lysosomal hydrolases from Dictyostelium discoideum ( Freeze et al., 1984 ).…”

Section: Introductionmentioning

confidence: 99%

Hallmarks of the relationship between host and Trypanosoma cruzi sulfated glycoconjugates along the course of Chagas disease

Soprano

Ferrero²,

Jacobs

et al. 2023

Front. Cell. Infect. Microbiol.

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American Trypanosomiasis or Chagas disease (ChD), a major problem that is still endemic in large areas of Latin America, is caused by Trypanosoma cruzi. This agent holds a major antigen, cruzipain (Cz). Its C-terminal domain (C-T) is retained in the glycoprotein mature form and bears several post-translational modifications. Glycoproteins containing sulfated N-linked oligosaccharides have been mostly implicated in numerous specific procedures of molecular recognition. The presence of sulfated oligosaccharides was demonstrated in Cz, also in a minor abundant antigen with serine-carboxypeptidase (SCP) activity, as well as in parasite sulfatides. Sulfate-bearing glycoproteins in Trypanosomatids are targets of specific immune responses. T. cruzi chronically infected subjects mount specific humoral immune responses to sulfated Cz. Unexpectedly, in the absence of infection, mice immunized with C-T, but not with sulfate-depleted C-T, showed ultrastructural heart anomalous pathological effects. Moreover, the synthetic anionic sugar conjugate GlcNAc6SO3-BSA showed to mimic the N-glycan-linked sulfated epitope (sulfotope) humoral responses that natural Cz elicits. Furthermore, it has been reported that sulfotopes participate via the binding of sialic acid Ig-like-specific lectins (Siglecs) to sulfosialylated glycoproteins in the immunomodulation by host–parasite interaction as well as in the parasite infection process. Strikingly, recent evidence involved Cz-sulfotope-specific antibodies in the immunopathogenesis and infection processes during the experimental ChD. Remarkably, sera from chronically T. cruzi-infected individuals with mild disease displayed higher levels of IgG2 antibodies specific for sulfated glycoproteins and sulfatides than those with more severe forms of the disease, evidencing that T. cruzi sulfotopes are antigenic independently of the sulfated glycoconjugate type. Ongoing assays indicate that antibodies specific for sulfotopes might be considered biomarkers of human cardiac ChD progression, playing a role as predictors of stability from the early mild stages of chronic ChD.

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Immobilization of invertase on sepharose-linked enzyme glycosyl recognizing polyclonal antibodies

Jafri

Saleemuddin

1997

Biotechnol. Bioeng.

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Sulfated oligosaccharides block antibodies to many Dictyostelium discoideum acid hydrolases.

Cited by 42 publications

References 18 publications

Effects of chlorate on the sulfation process of Trypanosoma cruzi glycoconjugates. Implication of parasite sulfates in cellular invasion

Effects of chlorate on the sulfation process of Trypanosoma cruzi glycoconjugates. Implication of parasite sulfates in cellular invasion

Hallmarks of the relationship between host and Trypanosoma cruzi sulfated glycoconjugates along the course of Chagas disease

Immobilization of invertase on sepharose-linked enzyme glycosyl recognizing polyclonal antibodies

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