Sulfated modification enhances the immunomodulatory effect of Cyclocarya paliurus polysaccharide on cyclophosphamide-induced immunosuppressed mice through MyD88-dependent MAPK/NF-κB and PI3K-Akt signaling pathways

Yu, Yue; Mo, Shiru; Shen, Mingyue; Chen, Yi; Yu, Qiang; Li, Zhedong; Xie, Mingyong

doi:10.1016/j.foodres.2021.110756

Cited by 36 publications

(25 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the effects of MRP on the secretion of IL-6, IL-1β, TNF-α, IgA, IgM, and IgG in serum were evaluated. The serum levels of IL-6, IL-1β, TNF-α, IgA, IgM, and IgG significantly decreased after treatment with CTX ( Figure 6 ; P < 0.01), confirming findings of previous research ( 34 , 36 ). When treated with MRP, the levels of the cytokines and immunoglobulins were significantly enhanced compared with the model ( P < 0.05 or 0.01), indicating that MRP could attenuate immunosuppression in mice by increasing the production of these proteins.…”

Section: Resultssupporting

confidence: 89%

See 1 more Smart Citation

Effects of low molecular weight peptides from monkfish (Lophius litulon) roe on immune response in immunosuppressed mice

et al. 2022

View full text Add to dashboard Cite

This study aimed to investigate the immunomodulatory activation of low-molecular-weight peptides from monkfish (Lophius litulon) roe (named MRP) on cyclophosphamide (CTX)-induced immunosuppressed mice. Our results indicated that MRP (100 mg/kg/d BW) could significantly increase the body weight and immune organ index, and improve the morphological changes in the spleen and thymus of mice. These effects subsequently enhance the serum levels of interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF)-α, and immunoglobulin (Ig) A, IgM, and IgG. Furthermore, MRP could also improve CTX-induced oxidative stress, and activate the NF-κB and MAPK pathways in the spleen tissues. The findings reported herein indicate that MRP has a good immunomodulatory activation toward immunosuppressed mice, hence can potentially be developed as an immune adjuvant or functional food.

show abstract

Section: Resultssupporting

confidence: 89%

“…H&E staining was used to observe morphological changes in immune organs ( 34 ) in mice's spleen and thymus ( Figure 5 ). When treated with CTX, the boundary between the white and red-pulp of the spleen in the model was unclear, but the mature lymphocytes decreased with a certain amount of inflammatory cells.…”

Section: Resultsmentioning

confidence: 99%

Effects of low molecular weight peptides from monkfish (Lophius litulon) roe on immune response in immunosuppressed mice

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Meanwhile, LTA intervention resulted in the phosphorylation of NF-κB and inhibition of NF-κBp65 expression. TLRs are transmembrane proteins that recognize multiple types of pathogen-associated molecular patterns and serve as bridges between nonspecific and specific immunity. , MyD88 is a downstream protein of TLRs that mediates TLR-driven signaling pathways. TLRs recognize antigens and interact with MyD88 to initiate downstream signal transduction via NF-κB.…”

Section: Discussionmentioning

confidence: 99%

L-Theanine Modulates Intestine-Specific Immunity by Regulating the Differentiation of CD4+ T Cells in Ovalbumin-Sensitized Mice

Gong

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Ovalbumin (OVA), a common food protein, can cause deadly allergies with intestine-specific immune reactions. L-Theanine (LTA) shows great potential for regulating intestinal immunity. To investigate the regulatory effect of LTA intervention on intestine-specific immunity, a 41 day experiment was performed on BALB/c OVA-sensitized mice. The results show that injecting female mice intraperitoneally with 50 μg of OVA and administering 30 mg of OVA 4 times can successfully establish an OVAsensitized mouse model. LTA intervention significantly increased weight gain and thymus index (p < 0.05), decreased allergy and diarrhea scores (p < 0.05), and improved jejunum structure. Meanwhile, the histological score and degranulation of mast cells decreased. LTA intervention increased Clostridiales, Lachnospiraceae, Lactobacillus, Prevotella, and Ruminococcus abundance while decreasing Helicobacter abundance. Flow cytometry and Western blotting results indicated that 200 and 400 mg/kg of LTA upregulated the expression of T-bet and Foxp3 proteins (p < 0.05), thus promoting the differentiation of jejunum CD4+ T cells to Th1 and Tregs and increasing the cytokines IFN-γ, IL-10, and TGF-β (p < 0.05). We found that 200 and 400 mg/kg of LTA downregulated the expression of RORγt and GATA3, thus inhibiting the differentiation of Th2 and Th17 cells and decreasing cytokines IL-4, IL-5, IL-13 TNF-α, IL-6, and IL-17A (p < 0.05). LTA inhibited the degranulation of mast cells and significantly decreased the serum levels of OVA-IgE, HIS, and mouse MCPT-1 (p < 0.05). Therefore, LTA intervention alleviated OVA allergy by improving intestine-specific immunity.

show abstract

“…Cyclophosphamide (CTX) is a potent immunosuppressant widely used in treating several immune diseases and malignant tumors ( 5 , 6 ). However, numerous studies indicated that CTX could disrupt the DNA structure and immune cells and reduce normal lymphocytes and macrophages, thereby inhibiting cellular and humoral immune responses ( 7 , 8 ). On the other hand, long-term or high doses usage of CTX (80 or 100 mg/kg body weight (BW)) can cause intestinal injury and intestinal microbiota disruption ( 9 – 11 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of Fucoidan Isolated From Laminaria japonica on Immune Response and Gut Microbiota in Cyclophosphamide-Treated Mice

Tang

Zhao³

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

The effects of Laminaria japonica fucoidan (LF) on immune regulation and intestinal microflora in cyclophosphamide (CTX)-treated mice were investigated in this work. Results indicated that LF significantly enhanced the spleen and thymus indices, promoted spleen lymphocyte and peritoneal macrophages proliferation, and increased the immune-related cytokines production in serum. Moreover, LF could regulate intestinal flora composition, increasing the abundance of Lactobacillaceae and Alistipes, and inhibiting Erysipelotrichia, Turicibacter, Romboutsia, Peptostreptococcaceae, and Faecalibaculum. These results were positively correlated with immune characteristics. Overall, LF could be useful as a new potential strategy to mitigate CTX immunosuppression and intestinal microbiota disorders.

show abstract

Sulfated modification enhances the immunomodulatory effect of Cyclocarya paliurus polysaccharide on cyclophosphamide-induced immunosuppressed mice through MyD88-dependent MAPK/NF-κB and PI3K-Akt signaling pathways

Cited by 36 publications

References 49 publications

Effects of low molecular weight peptides from monkfish (Lophius litulon) roe on immune response in immunosuppressed mice

Effects of low molecular weight peptides from monkfish (Lophius litulon) roe on immune response in immunosuppressed mice

L-Theanine Modulates Intestine-Specific Immunity by Regulating the Differentiation of CD4+ T Cells in Ovalbumin-Sensitized Mice

Effects of Fucoidan Isolated From Laminaria japonica on Immune Response and Gut Microbiota in Cyclophosphamide-Treated Mice

Contact Info

Product

Resources

About