Sulfated modification, characterization and antitumor activities of Radix hedysari polysaccharide

Wei, Dongfeng; Wei, Yanxia; Chen, Weidong; Zhang, Lifeng

doi:10.1016/j.ijbiomac.2012.06.004

Cited by 55 publications

(18 citation statements)

References 17 publications

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“…The IR spectra of ACW0 and ACW0-Sul are shown in Figure 1. Comparing with the IR spectra of these two polysaccharide, we found two strong absorption bands at 1259.85 cm −1 and 844.75 cm −1 of ACW0-Sul, which were attributed to S=O and C-O-S stretching vibration according to previous reported literature [36]. It is known that substituents such as sulfate groups cause regular shifts in the 13 C NMR spectrum of signals of the nearest carbon atoms [37].…”

Section: Resultssupporting

confidence: 71%

A Novel Heterogalactan from Antrodia camphorata and Anti-Angiogenic Activity of Its Sulfated Derivative

Ding

et al. 2017

Polymers

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A heterogalactan, named ACW0, was extracted from Antrodia camphorata and purified by anion exchange and gel permeation chromatography. It was composed of galactose (94.98%), traces of mannose (2.41%), and fucose (2.61%), with its molecular weight estimated to be 13.5 k Da. The polysaccharide ACW0 was shown to be a mannofucogalactan with a backbone chain of α-D-1,6-linked Gal, attached by a non-reducing terminal α-D-Man and α-L-Fuc on C-2 of nearly every six α-D-1,6-linked Gal residues. A sulfated polysaccharide, ACW0-Sul was achieved by the chlorosulfonic acid-pyridine method. Compared with the native polysaccharide, ACW0-Sul could disrupt tube formation and migration as well as cell growth of human microvascular endothelial cells (HMEC-1) dose-dependently. Further studies revealed that phosphorylation of Extracellular Regulated Protein Kinases (Erk) and Focal Adhesion Kinase (FAK) were significantly inhibited by ACW0-Sul. These results suggested that ACW0-Sul could be a potent candidate for anti-angiogenic agent development.

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Section: Resultssupporting

confidence: 71%

A Novel Heterogalactan from Antrodia camphorata and Anti-Angiogenic Activity of Its Sulfated Derivative

Ding

et al. 2017

Polymers

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“…On the contrary, polysaccharides with poor solubility should be dissolved in the ionic liquid before mixing it with CSA‐pyridine complex; the next steps resemble the CSA‐pyridine method. The advantages of this method are that the yield and degree of substitution are ideal, and it is easier to obtain sulfated derivatives (Nguyen and others ; Wei and others ). But the disadvantages of this method are quite obvious as CAS has strong oxidizing and stimulating effects, and also a long reaction time which may seriously damage the structure of the polysaccharide and make the operation more complicated.…”

Section: Major Methods Of Molecular Modification Of Polysaccharidesmentioning

confidence: 99%

Molecular Modification of Polysaccharides and Resulting Bioactivities

Xiong

Lai

et al. 2015

Comp Rev Food Sci Food Safe

398

172

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Polysaccharides are ideal natural resources for supplements and pharmaceuticals that have received more and more attention over the years. Natural polysaccharides have been shown to have fewer side effects, but because of their inherently physicochemical properties, their bioactivities were difficult to compare with those of synthetic drugs. Thus, researchers have modified the structures and properties of natural polysaccharides based on structure-activity relationships and have obtained better functionally improved polysaccharides. This review focuses on the major modification methods of polysaccharides, and discusses the effect of molecular modification on their physicochemical properties and bioactivities. Molecular modification methods mainly include chemical, physical, and biological changes. Chemical modification is the most widely used method; it can significantly increase the water solubility and bioactivities of polysaccharides by grafting onto other groups. Physical and biological modifications only change the molecular weight of a polysaccharide, and thereby change its physicochemical properties and bioactivities. Most of the molecular modifications bring about an increase in the antioxidant activity of polysaccharides, and among these, sulfated and acetylated modifications are very common. Furthermore, phosphorylation modification is the most common application to increase antitumor activity, and modified polysaccharides have been shown to have anti-HIV activity as the result of sulfated modification.

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“…The RHP was purified by the Institute of Combined Traditional Chinese and Western Medicine, School of Basic Medical Science at Lanzhou University (Lanzhou, China) (6,9). The polysaccharide content was 97.3%.…”

Section: Methodsmentioning

confidence: 99%

Radix Hedysari polysaccharide suppresses lipid metabolism dysfunction in a rat model of non-alcoholic fatty liver disease via adenosine monophosphate-activated protein kinase pathway activation

Sun

Wang

Duan

et al. 2014

Molecular Medicine Reports

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Oxidative stress and excess hepatic lipid accumulation contribute to non-alcoholic fatty liver disease. Radix Hedysari polysaccharides (RHP) have attracted interest due to their antioxidant properties and immunomodulatory effects. However, the effect of RHP on hepatic lipid metabolism remains to be elucidated. In the present study, the response of Sprague-Dawley rat livers to a high-fat diet and RHP treatment was investigated by evaluating body weight, liver histology, hepatic lipid content, adenosine monophosphate-activated protein kinase (AMPK) activity and lipid metabolism gene transcriptional profiles. The present study demonstrated that RHP ameliorated lipid metabolism disorders, regulated hepatic lipid content, improved liver inflammation and damage, activated AMPK via phosphorylation, upregulated peroxisome proliferator-activated receptor α and downregulated the mRNA expression of sterol regulatory element binding protein-1c in rat livers, which reduced lipogenesis and increased lipolysis. Taken together, these results suggested that RHP effectively ameliorates lipid metabolism disorders in rat livers; thus, RHP may be a potential therapeutic agent in the prevention of hepatic steatosis.

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Sulfated modification, characterization and antitumor activities of Radix hedysari polysaccharide

Cited by 55 publications

References 17 publications

A Novel Heterogalactan from Antrodia camphorata and Anti-Angiogenic Activity of Its Sulfated Derivative

A Novel Heterogalactan from Antrodia camphorata and Anti-Angiogenic Activity of Its Sulfated Derivative

Molecular Modification of Polysaccharides and Resulting Bioactivities

Radix Hedysari polysaccharide suppresses lipid metabolism dysfunction in a rat model of non-alcoholic fatty liver disease via adenosine monophosphate-activated protein kinase pathway activation

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