Sulfated modification and antioxidant activity of exopolysaccahrides produced by Enterobacter cloacae Z0206

Jin, Mingliang; Lu, Zeqing; Huang, Ming; Wang, Youming; Wang, Yizhen

doi:10.1016/j.ijbiomac.2011.01.023

Cited by 47 publications

(16 citation statements)

References 35 publications

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“…Meanwhile, the sulfation caused moderate 1.7-fold potentiation of the anti-oxidative activity (Figure 3-A; Table 1). This can be attributed to the addition of electron-withdrawing groups by sulfation causing weaker dissociation energy of O-H bonds in LMWLs, as have been suggested for other sulfated polysaccharides in the anti-oxidative activities [32,33]. As an antioxidant, CDSO3 was more potent (4.8-fold) than the monomer CA (Table 1) as well as anti-oxidative vitamin C and E or Trolox ® .…”

Section: Discussionmentioning

confidence: 63%

Novel low molecular weight lignins as potential anti-emphysema agents: In vitro triple inhibitory activity against elastase, oxidation and inflammation

Saluja

Thakkar

et al. 2013

Pulmonary Pharmacology & Therapeutics

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No molecule has been found to be effective against emphysema to date primarily because of its complex pathogenesis that involves elastolysis, oxidation and inflammation. We here describe novel unsulfated or sulfated low molecular weight lignins (LMWLs) chemo-enzymatically prepared from 4-hydroxycinnamic acids monomers, as the first potent triple-action inhibitors of neutrophil elastase, oxidation and inflammation. The inhibitory potencies of three different cinnamic acid-based LMWLs were determined in vitro using chromogenic substrate hydrolysis assays, radical scavenging and lung cellular oxidative biomarker reduced glutathione (rGSH) assays, and lung cellular inflammatory biomarker NFκB and IL-8 assays, respectively. Each LWML uniquely displayed triple-action inhibition, among which CDSO3, a sulfated caffeic acid-based LMWL, was most potent. The half-maximal anti-human neutrophil elastase (HNE) potency of CDSO3 was 0.43 μM. This high potency arose from lignin-like oligomerization, which was further potentiated by 6.6-fold due to sulfation. Mechanistically, this elastase inhibition was of mixed-type, time-dependent and more selective to positively charged elastases. The half-maximal anti-oxidative potency of CDSO3 was 3.52 μM, 4.8-fold potentiated from that of the monomer, caffeic acid (CA). In contrast, the half-maximal inhibitory potency to TNFα-induced inflammation was 5–10 μM, despite no activity with the monomer. More intriguingly, this anti-inflammatory activity was essentially identical with different stimuli, okadaic acid and hydrogen peroxide (H2O2), which implied that CDSO3 acts directly on inflammatory cascades within the cells. Overall, oligomerization and sulfation produced or significantly potentiated the activity, in comparison to the monomer. Thus, sulfated and unsulfated LMWLs are novel non-peptidic 2.8–4.1 kDa macromolecules that exhibit for the first time potent triple inhibitory activity against elastase, oxidation and inflammation, the three major pathogenic mechanisms known to cause emphysema.

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Section: Discussionmentioning

confidence: 63%

Novel low molecular weight lignins as potential anti-emphysema agents: In vitro triple inhibitory activity against elastase, oxidation and inflammation

Saluja

Thakkar

et al. 2013

Pulmonary Pharmacology & Therapeutics

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“…There are concentrated sulfuric acid method, chlorosulfonic acid-pyridine (CSA-Pry) method, sulfur trioxide-pyridine and so on, in which CSA-Pry method is commonly used since the reagent preparation is easy, reaction condition is simple and production recovery is convenient. The main affection factors of CSA-Pry method include CSA-Pry ratio, reaction temperature and reaction time [11,12]. Ophiopogonis japonicus (Radix Ophiopogonis Japonici) is a traditional Chinese medicine with the function of nourishing yin and moisturizing lung.…”

Section: Abbreviationsmentioning

confidence: 99%

The optimization of sulfation modification conditions for ophiopogonpolysaccharide based on antiviral activity

Zhang

Wang

et al. 2012

International Journal of Biological Macromolecules

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“…This inflammation is claimed to be the connection between insulin resistance, obesity and diabetes (20). Our previous studies have demonstrated, that EPS has an immunomodulatory and antioxidant effect (14,21) and notably, administration of EPS resulted in a reduction of blood glucose levels and showed significant anti-inflammatory and anti diabetic effects (12,13). Thus, EPS was used as a therapeutic drug for the HFD-induced diabetes of KKAy mice in the present experiment.…”

Section: Discussionmentioning

confidence: 60%

Selenium-enriched exopolysaccharides produced by Enterobacter cloacae Z0206 alleviate adipose inflammation in diabetic KKAy mice through the AMPK/SirT1 pathway

Zhou

Wang

Yang

et al. 2013

Molecular Medicine Reports

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Abstract. Polysaccharides belong to a structurally diverse class of macromolecules, with the necessary flexibility for the precise regulatory mechanisms and high capacity for carrying biological information. On the basis of a previous study regarding the administration of selenium-enriched exopolysaccharides (Se-ECZ-EPS) produced by Enterobacter cloacae (E. cloacae) Z0206 which resulted in a reduction of blood glucose levels and showed significant anti-inflammatory and anti-diabetic effects, the present study was conducted to evaluate the effects and mechanism of EPS on the alleviation of fat inflammation in high-fat-diet (HFD) induced-diabetic KKAy mice. The HFD induced-diabetic KKAy mice were gavaged once daily with EPS (0.2 mg/g body weight) or distilled water, while the C57BL/6J mice were gavaged with distilled water. Six weeks later visceral adipose tissue (VAT) was collected for quantified polymerase chain reaction (qPCR) and western blot (WB) analysis. The results showed that following supplementation with EPS, interleukin (IL) 6, IL1β and tumor necrosis factor (TNF) α mRNA expression in VAT were significantly reduced, while Glut4, pAMPK and SirT1 protein expression were markedly increased when compared with KKAy mice gavaged with water. Furthermore, ATGL and HSL mRNA were also significantly decreased. Subsequently, 3T3-L1 adipocytes were treated with insulin to induce insulin resistance to determine the mechanism by which EPS affects inflammation. Following the treatment of adipocytes with 100 nM insulin for 8 h, IL6 and TNFα mRNA expression were significantly increased, while the content of glucose uptake and Glut4 protein expression were significantly decreased. When treated with 100 nM insulin and 0.1 mg/ml EPS, no significant change in IL6 and TNFα mRNA expression or glucose uptake were observed. However, when SirT1-siRNA or AMPKα1-siRNA was tranfected into the 3T3-L1 adipocytes prior to treatment with insulin and EPS, there was a significant increase in IL6 and TNFα mRNA abundance. In conclusion, VAT inflammation and lipolysis in HFD-induced KKAy mice were significantly decreased following EPS usage. Moreover, EPS may alleviate VAT inflammation primarily through the AMPK/SirT1 pathway.

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Sulfated modification and antioxidant activity of exopolysaccahrides produced by Enterobacter cloacae Z0206

Cited by 47 publications

References 35 publications

Novel low molecular weight lignins as potential anti-emphysema agents: In vitro triple inhibitory activity against elastase, oxidation and inflammation

Novel low molecular weight lignins as potential anti-emphysema agents: In vitro triple inhibitory activity against elastase, oxidation and inflammation

The optimization of sulfation modification conditions for ophiopogonpolysaccharide based on antiviral activity

Selenium-enriched exopolysaccharides produced by Enterobacter cloacae Z0206 alleviate adipose inflammation in diabetic KKAy mice through the AMPK/SirT1 pathway

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