Sulfated glycosaminoglycan-like polymers are present in an acidophilic biofilm from a sulfidic cave

Bruin, Stefan de; Sarbu, Serban M.; Meysman, Filip J. R.; Sousa, D. Z.; Loosdrecht, Mark C.M. van; Lin, Yue

doi:10.1016/j.scitotenv.2022.154472

Cited by 14 publications

(14 citation statements)

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“…12 de Bruin et al determined that the maximum content of GAGs-like polymers was ∼5.5% of EPS in the microbes of collected biofilms. 16 Thus, the current study is the first to demonstrate the occurrence of amino sugars in WAS, indicating the non-negligible role of amino sugar degradation in WAS fermentation. The genera norank_f__Caldilineaceae (6.0%), Lactococcus (5.5%), and Mycobacterium (3.8%) were identified as the dominant bacteria in WAS by high-throughput sequencing (Figure 1C).…”

Section: Occurrence Of Amino Sugars Identified In Eps Of Wasmentioning

confidence: 62%

“…The occurrence of amino sugars in EPS of WAS can be determined by combining chemical and metagenomic analysis. 4,13,16 Specifically, combining high-throughput sequencing and metagenomic analysis can be further used to reveal the key bacteria in WAS for amino sugar production. 10,17 As the second most abundant biomass in nature (>10 billion tons in aquatic environments annually), chitin is a structural polymer of GlcNAc in numerous marine invertebrates, fungi, and algae.…”

Section: Introductionmentioning

confidence: 99%

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Unveiling the Occurrence and Non-Negligible Role of Amino Sugars in Waste Activated Sludge Fermentation by an Enriched Chitin-Degradation Consortium

Wang,

Zhu,

Hong

et al. 2023

Environ. Sci. Technol.

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Polysaccharides in extracellular polymeric substances (EPS) can form a hybrid matrix network with proteins, impeding waste-activated sludge (WAS) fermentation. Amino sugars, such as N-acetyl-D-glucosamine (GlcNAc) polymers and sialic acid, are the non-negligible components in the EPS of aerobic granules or biofilm. However, the occurrence of amino sugars in WAS and their degradation remains unclear. Thus, amino sugars (∼6.0%) in WAS were revealed, and the genera of Lactococcus and Zoogloea were identified for the first time. Chitin was used as the substrate to enrich a chitin-degrading consortium (CDC). The COD balances for methane production ranged from 83.3 and 95.1%. Chitin was gradually converted to oligosaccharides and GlcNAc after dosing with the extracellular enzyme. After doing enriched CDC in WAS, the final methane production markedly increased to 60.4 ± 0.6 mL, reflecting an increase of ∼62%. Four model substrates of amino sugars (GlcNAc and sialic acid) and polysaccharides (cellulose and dextran) could be used by CDC. Treponema (34.3%) was identified as the core bacterium via excreting chitinases (EC 3.2.1.14) and N-acetyl-glucosaminidases (EC 3.2.1.52), especially the genetic abundance of chitinases in CDC was 2.5 times higher than that of WAS. Thus, this study provides an elegant method for the utilization of amino sugar-enriched organics.

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Section: Occurrence Of Amino Sugars Identified In Eps Of Wasmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Unveiling the Occurrence and Non-Negligible Role of Amino Sugars in Waste Activated Sludge Fermentation by an Enriched Chitin-Degradation Consortium

Wang,

Zhu,

Hong

et al. 2023

Environ. Sci. Technol.

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“…The latter are usually enriched in sulfated glycosaminoglycans (sGAG), negatively charged, highly hydrophilic polymers. Recently, strong evidence was brought that sGAGs-like polymers in biofilms from Sulfur Cave (Romania) can be directly produced by prokaryotes ( De Bruin et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Diversity, distribution and organic substrates preferences of microbial communities of a low anthropic activity cave in North-Western Romania

et al. 2023

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IntroductionKarst caves are characterized by relatively constant temperature, lack of light, high humidity, and low nutrients availability. The diversity and functionality of the microorganisms dwelling in caves micro-habitats are yet underexplored. Therefore, in-depth investigations of these ecosystems aid in enlarging our understanding of the microbial interactions and microbially driven biogeochemical cycles. Here, we aimed at evaluating the diversity, abundance, distribution, and organic substrate preferences of microbial communities from Peștera cu Apă din Valea Leșului (Leșu Cave) located in the Apuseni Mountains (North-Western Romania).Materials and MethodsTo achieve this goal, we employed 16S rRNA gene amplicon sequencing and community-level physiological profiling (CLPP) paralleled by the assessment of environmental parameters of cave sediments and water.Results and DiscussionPseudomonadota (synonym Proteobacteria) was the most prevalent phylum detected across all samples whereas the abundance detected at order level varied among sites and between water and sediment samples. Despite the general similarity at the phylum-level in Leșu Cave across the sampled area, the results obtained in this study suggest that specific sites drive bacterial community at the order-level, perhaps sustaining the enrichment of unique bacterial populations due to microenvironmental conditions. For most of the dominant orders the distribution pattern showed a positive correlation with C-sources such as putrescine, γ-amino butyric acid, and D-malic acid, while particular cases were positively correlated with polymers (Tween 40, Tween 80 and α-cyclodextrin), carbohydrates (α-D-lactose, i-erythritol, D-mannitol) and most of the carboxylic and ketonic acids. Physicochemical analysis reveals that sediments are geochemically distinct, with increased concentration of Ca, Fe, Al, Mg, Na and K, whereas water showed low nitrate concentration. Our PCA indicated the clustering of different dominant orders with Mg, As, P, Fe, and Cr. This information serves as a starting point for further studies in elucidating the links between the taxonomic and functional diversity of subterranean microbial communities.

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“…These differences could be caused by the presence of binding sites other than NulOs in the EPS. For instance, sulfated polysaccharides have been described in the EPS of aerobic and anaerobic granular sludge (de Bruin et al, 2022; Felz et al, 2020), which could potentially contribute to the histone binding capacity of EPS. Further research is needed to examine all the histone binding sites in the EPS and the fractions, in order to fully understand the neutralization mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Enrichment and application of bacterial sialic acids containing polymers from the extracellular polymeric substances of “Candidatus Accumulibacter”

Tomás-Martínez

Chen

Pabst

et al. 2022

Preprint

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Pseudaminic and legionaminic acids are a subgroup of nonulosonic acids (NulOs) unique to bacterial species. There is a lack of advances in the study of these NulOs due to their complex synthesis and production. Recently, it was seen that Candidatus Accumulibacter can produce Pse or Leg analogues as part of its extracellular polymeric substances (EPS). In order to employ a Ca. Accumulibacter enrichment as production platform for bacterial sialic acids, it is necessary to determine which fractions of the EPS of Ca. Accumulibacter contain NulOs and how to enrich and/or isolate them. We extracted the EPS from granules enriched with Ca. Accumulibcater and used size-exclusion chromatography to separate them into different molecular weight fractions. This separation resulted in two high molecular weight (> 5,500 kDa) fractions dominated by polysaccharides, with a NulO content up to 4 times higher than the extracted EPS. This suggests that NulOs in Ca. Accumulibacter are likely located in high molecular weight polysaccharides. Additionally, it was seen that the extracted EPS and the NulO-rich fractions can bind and neutralize histones. This suggest that they can serve as source for sepsis treatment drugs, although further purification needs to be evaluated.

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Sulfated glycosaminoglycan-like polymers are present in an acidophilic biofilm from a sulfidic cave

Cited by 14 publications

References 37 publications

Unveiling the Occurrence and Non-Negligible Role of Amino Sugars in Waste Activated Sludge Fermentation by an Enriched Chitin-Degradation Consortium

Unveiling the Occurrence and Non-Negligible Role of Amino Sugars in Waste Activated Sludge Fermentation by an Enriched Chitin-Degradation Consortium

Diversity, distribution and organic substrates preferences of microbial communities of a low anthropic activity cave in North-Western Romania

Enrichment and application of bacterial sialic acids containing polymers from the extracellular polymeric substances of “Candidatus Accumulibacter”

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