Sulfate‐reducing and methanogenic bacteria from deep aquifers in montana

Olson, Gregory J.; Dockins, William S.; McFeters, Gordon A.; Iverson, Warren P.

doi:10.1080/01490458109377772

Cited by 64 publications

(27 citation statements)

References 18 publications

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“…Consequently, it is likely that the bacteria that utilized lactate in the anaerobic incubations were the SRB. This suggests that SRB constitute a substantial part of the bacterial populations in the fractures of the Stripa crystalline bedrock, as has been reported for other deep geological formations (Olson et al, 1981 ;Pedersen & Ekendahl, 1990). Sulphate reduction by SRB will increase the 634S isotopic content of a groundwater due to the preference for 32S by sulphate reducers (Widdel, 1988) and also result in increasing amounts of sulphide.…”

Section: Discussionmentioning

confidence: 62%

Incorporation of CO2 and introduced organic compounds by bacterial populations in groundwater from the deep crystalline bedrock of the Stripa mine

Pedersen

Ekendahl

1992

Journal of General Microbiology

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~~The incorporation of C 0 2 and assimilation of introduced organic compounds by bacterial populations in deep groundwater from fractured crystalline bedrock has been studied. Three depth horizons of the subvertical borehole V2 in the Stripa mine, Sweden, 799-807m, 812-820m and 970-1240m, were sampled. The groundwaters, obtained from fracture systems without close hydraulic connections, were anoxic and had the following populations was demonstrated using microautoradiographic and liquid scintillation counting techniques. The measured C02 incorporation reflected the in sifu production of organic carbon from C02. Incorporation of formate followed that of C 0 2 and indicated the presence of bacteria able to substitute formate for C02, e.g. methanogenic bacteria. The presence of sulphate-reducing bacteria is suggested by the observed incorporation of lactate by up to 74% of the bacterial populations. The recorded uptake of glucose indicates the presence of heterotrophic bacteria other than sulphate-reducing bacteria. Up to 99 % of the populations incorporated leucine, showing that major fractions of the populations were viable.

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Section: Discussionmentioning

confidence: 62%

Incorporation of CO2 and introduced organic compounds by bacterial populations in groundwater from the deep crystalline bedrock of the Stripa mine

Pedersen

Ekendahl

1992

Journal of General Microbiology

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“…Several other studies have discovered evidence of Desulfotomaculum spp. and other sulphate-reducing bacteria in the deep subsurface (Baker et al, 2003;Detmers et al, 2004;Fredrickson et al, 1997;Havemann et al, 1999;Olson et al, 1981;Onstott et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Monitoring of the microbial community composition in saline aquifers during CO2 storage by fluorescence in situ hybridisation

Morozova¹,

Wandrey²,

Alawi³

et al. 2010

International Journal of Greenhouse Gas Control

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This study reveals the first analyses of the composition and activity of the microbial community of a saline CO 2 storage aquifer. Microbial monitoring during CO 2 injection has been reported. By using Fluorescence in situ Hybridisation (FISH), we have shown that the microbial community was strongly influenced by the CO 2 injection. Before CO 2 arrival, up to 6x10 6 cells ml -1 were detected by DAPI-staining at a depth of 647 m below the surface. The microbial community was dominated by the domain Bacteria that represented approximately 60 to 90 % of the total cell number, with Proteobacteria and Firmicutes as the most abundant phyla comprising up to 47 % and 45 % of the entire population, respectively. Both the total cell counts as well as the counts of the specific physiological groups revealed quantitative and qualitative changes after CO 2 arrival. Our study revealed temporal shifts in the microbial community from chemoorganotrophic to chemolithotrophic populations, as evidenced by the outcompetition of sulphate-reducing bacteria by methanogenic archaea. In addition, an enhanced activity of the microbial population after five months CO 2 storage indicated that the bacterial community was able to adapt to the extreme conditions of the deep biosphere and to the extreme changes of these atypical conditions.

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“…the dissolution and precipitation of minerals (Culver et al 2004;Malard et al 1994;Mathieu et al 1992;Northup and Lavoie 2001;Rouch and Danielopol 1997;SchmitterSoto et al 2002;Sket 1999;Smith and Wood 2002;Vervier and Gibert 1991;Wood et al 2002). Considerable research has also been done on microbial communities in extreme hydrogeological environments, such as hydrothermal fluids and deep aquifers (Ekendahl et al 1994;Olson et al 1981;Schulze-Makuch and Kennedy 2000).…”

Section: Introductionmentioning

confidence: 99%

Review: Microbial biocenoses in pristine aquifers and an assessment of investigative methods

2006

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The current knowledge of microbial biocenoses (communities) in pristine aquifers is presented in a review, which also discusses their relevance for questions of groundwater protection. Aquifers are heterogeneous on all scales and structured in a variety of habitats. The void spaces in many aquifers are small. The biocenoses are thus predominantly composed of microorganisms and, often, microinvertebrates. Larger voids and macroorganisms occur in karst cavities. Due to the absence of light, the biocenoses depend on chemical energy resources, which are, however, scarce in non-contaminated groundwater. The microorganisms thus show small cell sizes, low population densities and reduced activity; they developed specific strategies to survive oligotrophic conditions. The review also discusses the impact of contamination on the biocenoses, and the potential use of the biocenoses or specific organisms as indicators for groundwater quality, and the limits of this approach. Bacteria are either planktonic or attached to aquifer material, which requires both fluid and solid phase sampling. Most groundwater bacteria are viable but non-culturable. Consequently, cultivation techniques give an incomplete picture of the biocenoses, while methods from molecular microbiology provide genetic fingerprints of the entire community. Different analytical methods are available to count microorganisms, identify species, characterise microbial diversity, and measure activity.Résumé Cette revue expose l'état actuel des connaissances concernant les biocénoses microbiennes présentes dans les aquifères oligotrophes. L'impact d'une contamination sur les biocénoses est discuté, ainsi que le potentiel que Les bactéries sont planctoniques ou attachées aux matériaux de l'aquifère, ce qui demande unéchantillonnagè a la fois de l'eau et du substrat. De nombreuses méthodes sont aujourd'hui disponibles pour le comptage, l'identification et la caractérisation de la diversité, ainsi que la mesure des activités des organismes des aquifères. Comme la grande majorité des bactéries est viable mais non cultivable, les techniques de cultures actuelles ne donnent qu'une image incomplète des communautés, alors que les méthodes moléculaires développées récemment offrent la possibilité d'obtenir un profil de la communauté plus complet.Resumen Se presentauna reseña crítica del conocimiento actual de biocenosis microbiana (comunidades) en acuíferos prístinos la cual también discute su relevancia en términos de protección de aguas subterráneas. Los acuíferos son heterogéneos en todas las escalas y estructurados en una variedad de habitats. Los espacios vacíos en muchos acuíferos son pequeños. La biocenosis está por lo tanto compuesta predominantemente por microorganismos y, frecuentemente, microinvertebrados. Espacios más grandes y macroorganismos ocurren en cavidades kársticas. Debido a la ausencia de luz la biocenosis depende de recursos energéticos químicos los cuales, sin embargo, son escasos en agua subterránea no contaminada. Los microorganismos muestran...

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Sulfate‐reducing and methanogenic bacteria from deep aquifers in montana

Cited by 64 publications

References 18 publications

Incorporation of CO2 and introduced organic compounds by bacterial populations in groundwater from the deep crystalline bedrock of the Stripa mine

Incorporation of CO2 and introduced organic compounds by bacterial populations in groundwater from the deep crystalline bedrock of the Stripa mine

Monitoring of the microbial community composition in saline aquifers during CO2 storage by fluorescence in situ hybridisation

Review: Microbial biocenoses in pristine aquifers and an assessment of investigative methods

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