Sulfanilic acid increases intracellular free-calcium concentration, induces reactive oxygen species production and impairs trypsin secretion in pancreatic AR42J cells

Ameur, Fatma Zohra; Mehedi, Nabila; Khéroua, O.; Saïdi, D.; Salido, Ginés M.; Gonzalez, Antonio

doi:10.1016/j.fct.2018.07.001

Cited by 11 publications

(4 citation statements)

References 44 publications

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“…AR42J cells, derived from rat pancreatic acinar cells, have proven to be a valuable tool in various studies exploring pancreatic function, cell biology, and disease mechanisms [18,24,27]. In this study, AR42J cells treated with 9 mM of STC for 1 h showed a significant increase in intracellular Ca 2+ concentration, approximately a 50% reduction in cell viability, a decrease in MMP (Figure 3C), and heightened ROS production compared to untreated cells.…”

Section: Discussionmentioning

confidence: 55%

“…In the early stages of AP, intracellular Ca 2+ overload plays an important role, initiating inflammation and triggering the secretion of digestive enzymes such as amylase and lipase in pancreatic acinar cells [23]. Previous studies have established a close association between intracellular Ca 2+ overload, mitochondrial dysfunction, and the excessive production of ROS during AP progression [8,9,24,25]. The heightened ROS production further activates the NF-κB signaling pathway, initiating local inflammation in the pancreas by upregulating pro-inflammatory cytokines like TNF-α and IL-6 and initiating the NLRP3 inflammasome to trigger the inflammatory cascade in AP [26].…”

Section: Discussionmentioning

confidence: 99%

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Algal Oil Mitigates Sodium Taurocholate-Induced Pancreatitis by Alleviating Calcium Overload, Oxidative Stress, and NF-κB Activation in Pancreatic Acinar Cells

Fang,

Lin,

Chen

et al. 2024

CIMB

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Acute pancreatitis (AP) is characterized by elevated intracellular Ca2+ concentrations, mitochondrial dysfunction, and oxidative stress in pancreatic acinar cells. Algal oil (AO) has demonstrated antioxidant and anti-inflammatory properties. This study aims to explore the effects of algal oil on the microenvironment of AP. Rat pancreatic acinar AR42J cells were pretreated with AO containing 0, 50, 100, or 150 μM of docosahexaenoic acid (DHA) 2 h prior to AP induction using sodium taurocholate (STC). After 1 h of STC treatment, AR42J cells exhibited a significant increase in intracellular Ca2+ concentration and the production of amylase, lipase, reactive oxygen species, and pro-inflammatory mediators, including tumor necrosis factor-α and interleukin-6. These STC-induced increases were markedly reduced in cells pretreated with AO. In comparison to cells without AO, those treated with a high dose of AO before STC exposure demonstrated a significant increase in mitochondrial membrane potential and a decrease in lipid peroxidation. Furthermore, STC-activated nuclear factor kappa-B (NF-κB) was attenuated in AO-pretreated cells, as evidenced by a significant decrease in activated NF-κB. In conclusion, AO may prevent damage to pancreatic acinar cells by alleviating intracellular Ca2+ overload, mitigating mitochondrial dysfunction, reducing oxidative stress, and attenuating NF-κB-targeted inflammation.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Algal Oil Mitigates Sodium Taurocholate-Induced Pancreatitis by Alleviating Calcium Overload, Oxidative Stress, and NF-κB Activation in Pancreatic Acinar Cells

Fang,

Lin,

Chen

et al. 2024

CIMB

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“…In addition, research has focused on its potential to cause genetic mutations and cancer because it can be converted into an aromatic amine, sulfanilic acid, by the microorganisms in the gut [15]. Sufanilic acid has been shown to cause oxidative stress and cellular damage to human pancreatic cells, for instance [16].…”

Section: Introductionmentioning

confidence: 99%

Tartrazine Modifies the Activity of DNMT and HDAC Genes—Is This a Link between Cancer and Neurological Disorders?

Zand,

Enkhbilguun,

Macharia

et al. 2023

Nutrients

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In recent years, artificial additives, especially synthetic food colorants, were found to demonstrate wider properties compared to their natural equivalents; however, their health impact is still not totally mapped. Our study aimed to determine the long-term (30 and 90 days) exposure effect of one of the commonly used artificial food colorants, tartrazine, on NMRI mice. The applied dose of tartrazine referred to the human equivalent dose for acceptable daily intake (ADI). Further, we evaluated its impact on the transcription of a range of epigenetic effectors, members of the DNA methyltransferase (DNMT) as well as histone deacetylase (HDAC) families. Following the exposure, organ biopsies were collected from the lungs, kidneys, liver, and spleen, and the gene expression levels were determined by real-time quantitative reverse transcription PCR (RT-qPCR). Our results demonstrated significant upregulation of genes in the tested organs in various patterns followed by the intake of tartrazine on ADI. Since DNMT and HDAC genes are involved in different steps of carcinogenesis, have roles in the development of neurological disorders and the effect of dose of everyday exposure is rarely studied, further investigation is warranted to study these possible associations.

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“…Tartrazine is metabolized by the intestinal microflora into sulfanilic acid and aminopyrazolone-metabolites which generate reactive oxygen species (ROS), leading to oxidative stress. 6 Tartrazine induced oxidative stress damages rat brain tissue 7 and alters Ca 2+ signaling and enzyme secretion in pancreatic AR42J cells 8 via sulfanilic acid production. Rats fed on tartrazine show an inflammation of stomach lining.…”

Section: Introductionmentioning

confidence: 99%

Toxic Effects of Food Colorants Erythrosine and Tartrazine on Zebrafish Embryo Development

Gupta

Ranjan²,

Yadav

et al. 2019

Curr Res Nutr Food Sci

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Erythrosine and tartrazine are common artificial food additives which have become a part of daily human consumption. Advised daily intake values for these agents are set strictly, however, the actual intake is much higher than the recommended ADI. A higher intake of erythrosine and tartrazine is shown to exhibit adverse effects in mammalian models, and is thus a matter of public health concern. In this study we have assessed and compared the dose-dependent effects of erythrosine and tartrazine on inducing oxidative stress in zebrafish embryos. We performed the superoxide dismutase (SOD) enzyme activity assay to test the effect of the two food colorants on reactive oxygen species (ROS) production. Erythrosine and tartrazine treated embryos showed significantly increased SOD activity in an enzyme assay. Additionally SOD mRNA transcripts in the treated embryos were found to be upregulated. Erythrosine and tartrazine treatment specifically altered SOD1 mRNA transcript levels while it had no effect on SOD2 mRNA, the other isoform found in zebrafish. Our study shows that erythrosine at a concentration of 0.05% is embryotoxic in a dose and time dependent manner. Tartrazine treated embryos exhibit similar toxicity at a concentration of 0.5%. Erythrosine treated zebrafish embryos hatch much slower when compared to tartrazine treated embryos and control embryos. While erythrosine affects the yolk utilization, tartrazine exhibits teratogenic effects on early zebrafish embryos. The mRNA expression as well as biochemical analysis indicates that exposure to food colorants induces cytoplasmic SOD transcription to combat the ROS toxicity in zebrafish embryos.

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Sulfanilic acid increases intracellular free-calcium concentration, induces reactive oxygen species production and impairs trypsin secretion in pancreatic AR42J cells

Cited by 11 publications

References 44 publications

Algal Oil Mitigates Sodium Taurocholate-Induced Pancreatitis by Alleviating Calcium Overload, Oxidative Stress, and NF-κB Activation in Pancreatic Acinar Cells

Algal Oil Mitigates Sodium Taurocholate-Induced Pancreatitis by Alleviating Calcium Overload, Oxidative Stress, and NF-κB Activation in Pancreatic Acinar Cells

Tartrazine Modifies the Activity of DNMT and HDAC Genes—Is This a Link between Cancer and Neurological Disorders?

Toxic Effects of Food Colorants Erythrosine and Tartrazine on Zebrafish Embryo Development

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