Sulfamethoxazole persistence in a river water ecosystem and its effects on the natural microbial community and Lemna minor plant

Grenni, Paola; Patrolecco, Luisa; Rauseo, Jasmin; Spataro, Francesca; Lenola, Martina Di; Aimola, Giorgia; Zacchini, Massimo; Pietrini, Fabrizio; Baccio, D. Di; Stanton, Isobel; Gaze, William H.; Caracciolo, Anna Barra

doi:10.1016/j.microc.2019.103999

Cited by 34 publications

(8 citation statements)

References 69 publications

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“…These are properties, among others, that make these products a potential risk for biocenoses and environmental health once they are released into the aquatic environment. In addition, several studies demonstrated that drug residues in treated wastewater and surface water are widespread (e.g., Brain et al 2004a , b ; Fent et al 2006 ; Küster and Adler 2014 ; Godoy et al 2018 ; Grenni et al 2019 ) and once released in the environment they may have adverse effects on exposed biocenoses (Küster and Adler 2014 ; Alkimin et al 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Aquatic plants and ecotoxicological assessment in freshwater ecosystems: a review

Ceschin

Bellini

Scalici

2020

Environ Sci Pollut Res

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This paper reviews the current state-of-the-art, limitations, critical issues, and new directions in freshwater plant ecotoxicology. We selected peer-reviewed studies using relevant databases and for each (1) publication year, (2) test plant species, (3) reference plant group (microalgae, macroalgae, bryophytes, pteridophytes, flowering plants), (4) toxicant tested (heavy metal, pharmaceutical product, hydrocarbon, pesticide, surfactant, plastic), (5) experiment site (laboratory, field), and (6) toxicant exposure duration. Although aquatic plant organisms play a key role in the functioning of freshwater ecosystems, mainly linked to their primary productivity, their use as biological models in ecotoxicological tests was limited if compared to animals. Also, toxicant effects on freshwater plants were scarcely investigated and limited to studies on microalgae (80%), or only to a certain number of recurrent species (Pseudokirchneriella subcapitata, Chlorella vulgaris, Lemna minor, Myriophyllum spicatum). The most widely tested toxicants on plants were heavy metals (74%), followed by pharmaceutical products and hydrocarbons (7%), while the most commonly utilized endpoints in tests were plant growth inhibition, variations in dry or fresh weight, morpho-structural alterations, chlorosis, and/or necrosis. The main critical issues emerged from plant-based ecotoxicological tests were the narrow range of species and endpoints considered, the lack of environmental relevance, the excessively short exposure times, and the culture media potentially reacting with toxicants. Proposals to overcome these issues are discussed.

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Section: Resultsmentioning

confidence: 99%

Aquatic plants and ecotoxicological assessment in freshwater ecosystems: a review

Ceschin

Bellini

Scalici

2020

Environ Sci Pollut Res

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“…The replication of freshwater environments such as rivers in the laboratory is difficult and microcosms studies were usually performed on static or shaking systems which only partially resembled the natural environment dynamic characteristics (Grenni et al, 2019;Patrolecco et al, 2018;Tong and Xie, 2019;Vila-Costa et al, 2017). In the current study flumes were set up in closed in vitro systems where water flowed on a sediment bed to better mimic river and environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

Impact of sulfamethoxazole on a riverine microbiome

Borsetto

Raguideau

Travis

et al. 2021

Preprint

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The continued emergence of bacterial pathogens presenting antimicrobial resistance is widely recognised as a global health threat and recent attention focused on potential environmental reservoirs of antibiotic resistance genes (ARGs). Freshwater environments such as rivers represent a potential hotspot for ARGs and antibiotic resistant bacteria as they are receiving systems for effluent discharges from wastewater treatment plants (WWTPs). Effluent also contains low levels of different antimicrobials including antibiotics and biocides. Sulfonamides are antibacterial chemicals widely used in clinical, veterinary and agricultural settings and are frequently detected in sewage sludge and manure in addition to riverine ecosystems. The impact of such exposure on ARG prevalence and diversity is unknown, so the aim of this study was to investigate the release of a sub-lethal concentration of the sulfonamide compound sulfamethoxazole (SMX) on the river bacterial microbiome using a flume system. This system was a semi-natural in vitro flume using river water (30 L) and sediment with circulation to mimic river flow. A combination of 'omics' approaches were conducted to study the impact of SMX exposure on the microbiomes within the flumes. Metagenomic analysis showed that the addition of low concentrations of SMX (<4 μg L-1) had a limited effect on the bacterial resistome in the water fraction only, with no impact observed in the sediment. Metaproteomics did not show differences in ARGs expression with SMX exposure in water. Overall, the river bacterial community was resilient to short term exposure to sub-lethal concentrations of SMX which mimics the exposure such communities experience downstream of WWTPs throughout the year.

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“…Of 75 articles reviewed, 45 − ,− ,,,− detected 48 different antibiotic (bio)TPs in surface waters (Table S10). TPs from the parent compounds SMX − ,,,,,− ,− , were the most frequently reported (40%, 18 of 45 articles).…”

Section: Occurrences Of Tps In Surface Water Environmentsmentioning

confidence: 99%

Antimicrobial Transformation Products in the Aquatic Environment: Global Occurrence, Ecotoxicological Risks, and Potential of Antibiotic Resistance

Löffler

Escher

Baduel

et al. 2023

Environ. Sci. Technol.

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The global spread of antimicrobial resistance (AMR) is concerning for the health of humans, animals, and the environment in a One Health perspective. Assessments of AMR and associated environmental hazards mostly focus on antimicrobial parent compounds, while largely overlooking their transformation products (TPs). This review lists antimicrobial TPs identified in surface water environments and examines their potential for AMR promotion, ecological risk, as well as human health and environmental hazards using in silico models. Our review also summarizes the key transformation compartments of TPs, related pathways for TPs reaching surface waters and methodologies for studying the fate of TPs. The 56 antimicrobial TPs covered by the review were prioritized via scoring and ranking of various risk and hazard parameters. Most data on occurrences to date have been reported in Europe, while little is known about antibiotic TPs in Africa, Central and South America, Asia, and Oceania. Occurrence data on antiviral TPs and other antibacterial TPs are even scarcer. We propose evaluation of structural similarity between parent compounds and TPs for TP risk assessment. We predicted a risk of AMR for 13 TPs, especially TPs of tetracyclines and macrolides. We estimated the ecotoxicological effect concentrations of TPs from the experimental effect data of the parent chemical for bacteria, algae and water fleas, scaled by potency differences predicted by quantitative structure–activity relationships (QSARs) for baseline toxicity and a scaling factor for structural similarity. Inclusion of TPs in mixtures with their parent increased the ecological risk quotient over the threshold of one for 7 of the 24 antimicrobials included in this analysis, while only one parent had a risk quotient above one. Thirteen TPs, from which 6 were macrolide TPs, posed a risk to at least one of the three tested species. There were 12/21 TPs identified that are likely to exhibit a similar or higher level of mutagenicity/carcinogenicity, respectively, than their parent compound, with tetracycline TPs often showing increased mutagenicity. Most TPs with increased carcinogenicity belonged to sulfonamides. Most of the TPs were predicted to be mobile but not bioaccumulative, and 14 were predicted to be persistent. The six highest-priority TPs originated from the tetracycline antibiotic family and antivirals. This review, and in particular our ranking of antimicrobial TPs of concern, can support authorities in planning related intervention strategies and source mitigation of antimicrobials toward a sustainable future.

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Sulfamethoxazole persistence in a river water ecosystem and its effects on the natural microbial community and Lemna minor plant

Cited by 34 publications

References 69 publications

Aquatic plants and ecotoxicological assessment in freshwater ecosystems: a review

Aquatic plants and ecotoxicological assessment in freshwater ecosystems: a review

Impact of sulfamethoxazole on a riverine microbiome

Antimicrobial Transformation Products in the Aquatic Environment: Global Occurrence, Ecotoxicological Risks, and Potential of Antibiotic Resistance

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