Suitable Experimental Design for Determination of Auxin Polar Transport in Space Using a Spacecraft.

Shimazu, Toru; Miyamoto, Kazuaki; Hoson, Takayuki; Kamisaka, Seiichiro; Ueda, Junichi

doi:10.2187/bss.14.9

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…At the end of incubation, a 2-mm piece of the opposite side from the donor side was excised, and the radioactivity was measured directly using a liquid scintillation counter (2200CA, Packard Instrument, Meriden, CT). Almost all the radioactivity in the opposite side of the segments donated radiolabeled IAA in the segments resulted from that of [1-14 C]IAA transported within at least 16 h in planta (Oka et al 1995;Shimazu et al 2000). Each experiment was carried out using at least ten segments and was repeated three times.…”

Section: Measurement Of Polar Auxin Transportmentioning

confidence: 99%

Gravity-controlled asymmetrical transport of auxin regulates a gravitropic response in the early growth stage of etiolated pea (Pisum sativum) epicotyls: studies using simulated microgravity conditions on a three-dimensional clinostat and using an agravitropic mutant, ageotropum

2007

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Increased expression of the auxin-inducible gene PsIAA4/5 was observed in the elongated side of epicotyls in early growth stages of etiolated pea (Pisum sativum L. cv. Alaska) seedlings grown in a horizontal or an inclined position under 1 g conditions. Under simulated microgravity conditions on a 3D clinostat, accumulation of PsIAA4/5 mRNA was found throughout epicotyls showing automorphosis. Polar auxin transport in the proximal side of epicotyls changed when the seedlings were grown in a horizontal or an inclined position under 1 g conditions, but that under clinorotation did not, regardless of the direction of seed setting. Accumulation of PsPIN1 and PsPIN2 mRNAs in epicotyls was affected by gravistimulation, but not by clinorotation. Under 1 g conditions, auxin-transport inhibitors made epicotyls of seedlings grown in a horizontal or inclined position grow toward the proximal direction to cotyledons. These inhibitors led to epicotyl bending toward the cotyledons in seedlings grown in an inclined position under clinorotation. Polar auxin transport, as well as growth direction, of epicotyls of the agravitropic mutant ageotropum did not respond to various gravistimulation. These results suggest that alteration of polar auxin transport in the proximal side of epicotyls regulates the graviresponse of pea epicotyls.

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Section: Measurement Of Polar Auxin Transportmentioning

confidence: 99%

Gravity-controlled asymmetrical transport of auxin regulates a gravitropic response in the early growth stage of etiolated pea (Pisum sativum) epicotyls: studies using simulated microgravity conditions on a three-dimensional clinostat and using an agravitropic mutant, ageotropum

2007

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“…The growth and development of inflorescence axes of Arabidopsis thaliana observed under simulated microgravity conditions on a clinostat was concerned with the changes in their activities of auxin polar transport , Oka et al 1995, Ueda et al 1996, in which auxin moves basipetally in plants. Moreover, auxin polar transport in etiolated pea epicotyls, and maize coleoptiles and mesocotyls was substantially influenced under simulated microgravity conditions on a 3-D clinostat (Shimazu et al 2000, Ueda et al 1999a. Etiolated pea and maize seedlings have also been shown to be suitable plant materials to investigate the effect of microgravity conditions in space on auxin polar transport (Ueda et al 1997).…”

Section: Introduction (Objective and Background)mentioning

confidence: 99%

STS-95 space experiment for plant growth and development, and auxin polar transport.

et al. 2000

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The principal objective of the space experiment, BRIC-AUX on STS-95, was the integrated analysis of the growth and development of etiolated pea and maize seedlings in space, and the effect of microgravity conditions in space on auxin polar transport in the segments. Microgravity conditions in space strongly affected the growth and development of etiolated pea and maize seedlings. Etiolated pea and maize seedlings were leaned and curved during space flight, respectively. Finally the growth inhibition of these seedlings was also observed. Roots of some pea seedlings grew toward the aerial space of Plant Growth Chamber. Extensibilities of cell walls of the third internode of etiolated pea epicotyls and the top region of etiolated maize coleoptiles which were germinated and grown under microgravity conditions in space were significantly low. Activities of auxin polar transport in the second internode segments of etiolated pea seedlings and coleoptile segments of etiolated maize seedlings were significantly inhibited and extremely promoted, respectively, under microgravity conditions in space. These results strongly suggest that auxin polar transport as well as the growth and development of plants is controlled under gravity on the earth.

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Requirement for the Gravity-Controlled Transport of Auxin for a Negative Gravitropic Response of Epicotyls in the Early Growth Stage of Etiolated Pea Seedlings

Hoshino

Miyamoto

Ueda

2006

Plant and Cell Physiology

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Gravity-controlled transport of auxin was studied for a negative gravitropic response in the early growth stage of etiolated pea (Pisum sativum L. cv. Alaska) seedlings, in which epicotyl bending was observed near the cotyledon nodes of the seedlings grown continuously from seeds germinated in a horizontal or an inclined position. Increased expression of an auxin-inducible gene, PsIAA4/5, was observed in the elongated side of epicotyls grown in a horizontal or an inclined position. Regardless of the conditions of seed germination, polar auxin transport in the proximal side of the first internodes of the seedlings was significantly higher than in the distal side. Polar auxin transport in the proximal side of epicotyls grown in an inclined position was significantly lower than in those grown in a horizontal position. In contrast, lateral auxin distribution from the proximal to distal sides in epicotyls grown in an inclined position was significantly higher than in epicotyls grown in a horizontal position. Accumulation of PsPIN1 mRNA encoding a putative auxin efflux facilitator, which was observed in vascular tissue, cortex and epidermis in the proximal and distal sides of epicotyls, was markedly influenced by gravistimulation. These results strongly suggest that gravistimulation induces changeable polar auxin transport and one-way lateral auxin distribution in epicotyls as well as asymmetric auxin accumulation in the proximal and distal sides of epicotyls, resulting in a negative gravitropic response of epicotyls in the early growth stage of pea seedlings.

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Suitable Experimental Design for Determination of Auxin Polar Transport in Space Using a Spacecraft.

Cited by 3 publications

References 13 publications

Gravity-controlled asymmetrical transport of auxin regulates a gravitropic response in the early growth stage of etiolated pea (Pisum sativum) epicotyls: studies using simulated microgravity conditions on a three-dimensional clinostat and using an agravitropic mutant, ageotropum

Gravity-controlled asymmetrical transport of auxin regulates a gravitropic response in the early growth stage of etiolated pea (Pisum sativum) epicotyls: studies using simulated microgravity conditions on a three-dimensional clinostat and using an agravitropic mutant, ageotropum

STS-95 space experiment for plant growth and development, and auxin polar transport.

Requirement for the Gravity-Controlled Transport of Auxin for a Negative Gravitropic Response of Epicotyls in the Early Growth Stage of Etiolated Pea Seedlings

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