Suitability of culture media for the production of ascospores and maintenance of<i>Ascosphaera apis</i>

Ruffinengo, Sergio; Peña, Nora I.; Clemente, G.E.; Palacio, Marí­a Alejandra; Escande, A. R.

doi:10.1080/00218839.2000.11101034

Cited by 13 publications

(9 citation statements)

References 6 publications

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“…Many techniques have been used to cultivate A. apis for research purposes (Maurizio, 1934;Skou, 1972;Christensen and Gilliam, 1983;Bailey and Ball, 1991;Ruffinengo et al, 2000). In culture, A. apis can grow on a wide variety of media in either aerobic (Spiltoir, 1955;Heath, 1982b;Rose et al, 1984;Bissett, 1988;Gilliam et al, 1988b) or anaerobic conditions (Thomas and Luce, 1972;Heath and Gaze, 1987;Anderson et al, 1997;Johnson et al, 2005).…”

Section: Fungal Culture and Storage Conditionsmentioning

confidence: 97%

Chalkbrood disease in honey bees

Aronstein

Murray

2010

Journal of Invertebrate Pathology

213

146

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Section: Fungal Culture and Storage Conditionsmentioning

confidence: 97%

Chalkbrood disease in honey bees

Aronstein

Murray

2010

Journal of Invertebrate Pathology

213

146

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“…An A. apis isolate starts to show signs of aging after 30 days of growth (Ruffinengo et al ., 2000) and thus requires monthly transfer. However, frequent culture transfers are expensive, time consuming and increase the risk of contamination.…”

Section: Chalkbroodmentioning

confidence: 99%

“…From a standardization point of view, the use of in vitro spores from reference strains are strongly recommended, but spores retrieved from black mummies might be chosen in certain situations. Ascosphaera apis will mate on various media but the yield of spores is increased on malt yeast with 20% dextrose agar (MY20) (Ruffinengo et al ., 2000). It is recommended that a fresh spore solution be made for every experiment.…”

Section: Chalkbroodmentioning

confidence: 99%

Standard methods for fungal brood disease research

Jensen

Aronstein²,

Flores

et al. 2013

Journal of Apicultural Research

144

130

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Summary Chalkbrood and stonebrood are two fungal diseases associated with honey bee brood. Chalkbrood, caused by Ascosphaera apis, is a common and widespread disease that can result in severe reduction of emerging worker bees and thus overall colony productivity. Stonebrood is caused by Aspergillus spp. that are rarely observed, so the impact on colony health is not very well understood. A major concern with the presence of Aspergillus in honey bees is the production of airborne conidia, which can lead to allergic bronchopulmonary aspergillosis, pulmonary aspergilloma, or even invasive aspergillosis in lung tissues upon inhalation by humans. In the current chapter we describe the honey bee disease symptoms of these fungal pathogens. In addition, we provide research methodologies and protocols for isolating and culturing, in vivo and in vitro assays that are commonly used to study these host pathogen interactions. We give guidelines on the preferred methods used in current research and the application of molecular techniques. We have added photographs, drawings and illustrations to assist bee-extension personnel and bee scientists in the control of these two diseases.

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“…2B). An alternative for A. apis is to store cultures on rice kernels (Ruffinengo et al, 2000). Palacio et al (2007) found viability to be 73% for A. apis stored in this way for one year.…”

Section: Resultsmentioning

confidence: 98%