Suicide-Peroxide inactivation of microperoxidase-11: A kinetic study

Khosraneh, M.; Mahmoudi, Ali; Rahimi, H.; Nazari, Kamran; Moosavi-Movahedi, Ali Akbar

doi:10.1080/14756360701270683

Cited by 20 publications

(10 citation statements)

References 53 publications

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“…At low hydrogen peroxide concentrations, multiple additions led to a reproducible signal response. However, microperoxidases are known to be irreversibly inactivated by high concentrations of hydrogen peroxide . Therefore, concentration dependent reduction of hydrogen peroxide was measured using a flow‐cell system to decrease the risk of inactivation of MP‐11 by lowering the contact time with the substrate.…”

Section: Resultsmentioning

confidence: 99%

Bioelectrocatalytic Reduction of Hydrogen Peroxide by Microperoxidase‐11 Immobilized on Mesoporous Antimony‐Doped Tin Oxide

et al. 2017

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The heme‐undecapeptide microperoxidase‐11 (MP‐11) was immobilized on mesoporous antimony‐doped tin oxide (ATO) thin‐film electrodes modified with the positively charged binding promotor polydiallyldimethylammonium chloride. Surface concentrations of MP‐11 of 1.5 nmol cm−2 were sufficiently high to enable spectroelectrochemical analyses. UV/Vis spectroscopy and resonance Raman spectroscopy revealed that immobilized MP‐11 adopts a six‐coordinated low‐spin conformation, as in solution in the presence of a polycation. Cathodic reduction of hydrogen peroxide at potentials close to +500 mV versus Ag/AgCl indicates that the reaction proceeds via a Compound I‐type like intermediate, analogous to natural peroxidases, and confirms mesoporous ATO as a suitable host material for adsorbing the heme‐peptide in its native state. A hydrogen peroxide sensor is proposed by using the bioelectrocatalytic properties of the MP‐11‐modified ATO.

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Section: Resultsmentioning

confidence: 99%

Bioelectrocatalytic Reduction of Hydrogen Peroxide by Microperoxidase‐11 Immobilized on Mesoporous Antimony‐Doped Tin Oxide

et al. 2017

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“…For the other branch, hydrogen peroxide can in fact inhibit MP-11, though we are working well below the relevant concentrations. 87,88 Despite its obvious gross limitations as a realistic model of the biocatalytic processes involved, the present approach has advantages that make it practical for our purposes. It limits the number of fit parameters to reasonable (just two) for the quality of the available data.…”

Section: Model Of the Xor Response Surfacementioning

confidence: 99%

Realization and Properties of Biochemical-Computing Biocatalytic XOR Gate Based on Signal Change

et al. 2010

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We consider a realization of the XOR logic gate in a system involving two competing biocatalytic reactions, for which the logic-1 output is defined by these two processes causing a change in the optically detected signal. A model is developed for describing such systems in an approach suitable for evaluation of the analog noise amplification properties of the gate and optimization of its functioning. The initial data are fitted for gate quality evaluation within the developed model, and then modifications are proposed and experimentally realized for improving the gate functioning.

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“…In all hemoproteins, including peroxidases “suicide-peroxide inactivation” pathway initiates in high concentrations of hydrogen peroxide. A high concentration of hydrogen peroxide likewise can inactivate microperoxidases and then the peroxidases as a result of uncovered heme group [ 69 ]. When catalase meets H 2 O 2 , the bovine liver catalase reaction triggers.…”

Section: Enzyme Inhibitionmentioning

confidence: 99%

An outlook on suicide enzyme inhibition and drug design

2021

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Graphic abstract There have been recent renewed interests in the importance of suicide enzyme inhibition. The principal objective of this review is to investigate all types of suicide inhibitions for natural enzymes, artificial biocatalysts as well as therapeutic potential of enzyme suicide inhibition. It is discussed the suicide inhibition beneficial in drug design and treatments and non-beneficial achievements for some industrial enzymes such as HRP peroxidase enzyme. The design of biomimetic artificial enzymes explained to prevent inhibition by protecting the active site via environmental conditions. Suicide enzyme inhibition development can be the key mechanism against sever diseases such as SARS. In this report, suicide enzyme inactivation classes are classified based on target enzyme groups via their substrates. Suicide substrate deceives enzyme by its shape similarity to normal substrate and after interaction with enzyme, its suicidal functional group appears (e.g., Trojan horse).

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Suicide-Peroxide inactivation of microperoxidase-11: A kinetic study

Cited by 20 publications

References 53 publications

Bioelectrocatalytic Reduction of Hydrogen Peroxide by Microperoxidase‐11 Immobilized on Mesoporous Antimony‐Doped Tin Oxide

Bioelectrocatalytic Reduction of Hydrogen Peroxide by Microperoxidase‐11 Immobilized on Mesoporous Antimony‐Doped Tin Oxide

Realization and Properties of Biochemical-Computing Biocatalytic XOR Gate Based on Signal Change

An outlook on suicide enzyme inhibition and drug design

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