Sugars in the Blood and Urine of Children Following the Ingestion of Disaccharides

Haworth, J. C.

doi:10.1136/adc.35.184.552

Cited by 31 publications

(7 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the 38 controls only 13 showed sucrosuria after a loading dose. This figure is in accord with the findings of Haworth (1960). He reports that five out of 13 healthy babies and five out of 16 older children showed sucrosuria following the administration of a test dose.…”

Section: Methodssupporting

confidence: 89%

“…This was confirmed by Le Goff (1911) who administered 100 g. sugar to adults, all of whom developed temporary sucrosuria. Sucrosuria has been reported in children both in health as well as in those suffering from gastro-intestinal disturbances (Lahdensuu, 1947;Reuss, 1910;Owen and Lewis, 1956;Haworth, 1960). Sucrosuria has always been assumed to be secondary to the intake of sucrose with the exception of the cases reported by Elmer, Krasowska and Ptaszek (1939) and by Hoesch (1934).…”

mentioning

confidence: 99%

See 1 more Smart Citation

The Relation between Sucrosuria, Hiatus Hernia and Mental Defect

Freier¹

1962

Archives of Disease in Childhood

View full text Add to dashboard Cite

Section: Methodssupporting

confidence: 89%

mentioning

confidence: 99%

The Relation between Sucrosuria, Hiatus Hernia and Mental Defect

Freier¹

1962

Archives of Disease in Childhood

View full text Add to dashboard Cite

“…The studies by Haworth and McCredie [2], Woolf and Norman [3], and Haworth [4] demonstrated qualitatively that urine contained lactose fol lowing the ingestion of the disaccharide. However, there was no controlled quantita tive assessment of lactose excretion.…”

Section: Discussionmentioning

confidence: 99%

Intestinal Permeability to Intact Lactose in Newborns and Adults

et al. 1990

View full text Add to dashboard Cite

Small amounts of lactose have been shown to be absorbed intact across the intestine and excreted unchanged in the urine of newborns and adults. We designed a study to quantitate the intestinal uptake and urinary excretion of this disaccharide in these age groups. Similar amounts of lactose were given orally to 17 term newborns (age: 24.8 ± 3.0 h) as a standard infant formula, and to 15 adult lactose absorbers (age: 28.1 ± 2.6 years) and 11 adult lactose malabsorbers (age: 24.7 ± 2.9 years) as a 20% water solution. Following lactose ingestion, breath was collected every 30 or 60 min for 3 h and analyzed for hydrogen concentration. Urine was also collected, and lactose and creatinine concentrations were determined. Peak hydrogen concentration was < 20 ppm above baseline in newborns and adult lactose absorbers and 85 ± 14 ppm in adult lactose malabsorbers. Urinary lactose excretion, expressed as a function of body weight (mg/ml/kg b.w.), was substantially greater in newborns (4.2 ± 0.82) than in adult lactose absorbers (0.29 ± 0.07; p < 0.001) and adult lactose malabsorbers (0.55 ± 0.04; p < 0.01). Similarly, urinary lactose excretion expressed as a ratio of urinary lactose to urinary creatinine (mg/mg) was increased (p < 0.001) in newborns (2.05 ± 0.26) when compared to adult lactose absorbers (0.11 ± 0.02) and adult lactose malabsorbers (0.20 ± 0.02). Our data demonstrate that the intestinal uptake and urinary excretion of intact lactose is significantly increased in newborns compared to adult subjects.

show abstract

“…Although several types of direct measurement have been applied to zones separated by paper (PC) and thin Apparatus and materials layer chromatography (TLC) (Franglen, 1968), these techniques have not been regarded as sufficiently MATERIALS reliable (Bark, 1969) to justify the labour of sophisticated densitometry. Quantitative elution techniques are well established, but the preference shown by most authors for methods based on direct visual comparison of 'test' with 'standard' zones developed on the same chromatogram, which are at best semiquantitative (Owen and Lewis, 1956;Apthorp, 1957;Woodruff, 1958;Haworth, 1960;Bickel, 1961;Solvents Gryboski et al, 1963;Clarke et al, 1964;Launiala Ethyl acetate, pyridine, glacial acetic acid, and et al, 1964;Arthur et al, 1966) (Menzies and Mount, 1975), to examine the purity of all sugar preparations before selecting them for standards or internal markers.…”

Section: From the Department Of Chemical Pathology St Thomas's Hospimentioning

confidence: 99%

Quantitative Estimation of Clinically Important Monosaccharides in Plasma by Rapid Thin Layer Chromatography

1978

View full text Add to dashboard Cite

Summary A method is described for the quantitative estimation of clinically important monosaccharides in plasma or whole blood by direct densitometry of chromatographically-separated zones on silica gel layers. Simple modifications of technique originally introduced to improve the reproducibility of paper chromatography have now been adapted for thin layers. The present method is based on peak height measurement with an internal marker correction. Galactose, fructose, D-xylose, and 3-O-methyl glucose can be estimated in addition to glucose, either singly or in combination, within three or four hours, using an initial sample volume of 0·5 ml. With reasonable experience and skill a coefficient of variation of 3 to 6 %, depending on sugar concentration, can be achieved without replication, and the limit of sensitivity is about 0·05 mmol/l. When the performance was compared with an automated glucose oxidase/peroxidase system for glucose and the recovery for the other monosaccharides was calculated, the results were satisfactory.

show abstract

Sugars in the Blood and Urine of Children Following the Ingestion of Disaccharides

Cited by 31 publications

References 29 publications

The Relation between Sucrosuria, Hiatus Hernia and Mental Defect

The Relation between Sucrosuria, Hiatus Hernia and Mental Defect

Intestinal Permeability to Intact Lactose in Newborns and Adults

Quantitative Estimation of Clinically Important Monosaccharides in Plasma by Rapid Thin Layer Chromatography

Contact Info

Product

Resources

About