Sugaring-Out Assisted Liquid-Liquid Extraction Combined with High-Performance Liquid Chromatography-Fluorescence Detection for the Determination of Bisphenol A and Bisphenol B in Royal Jelly

Tu, Xijuan; Wu, Siyuan; Liu, Weiyi; Gao, Zhaosheng; Huang, Shaokang; Chen, Wenbin

doi:10.1007/s12161-018-1398-4

Cited by 19 publications

(8 citation statements)

References 42 publications

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“…Then the value of recovery further increased to 95.23% when the content of ACN reached 70:30 ( v / v , ACN:H 2 O). This observation was consistent with our previous reports on the sugaring-out extraction of 10-HDA [24] and bisphenol contaminants [27] in the royal jelly sample, in which the improvement of extraction yields can be attributed to the significant increase of phase ratio when the content of ACN increased.…”

Section: Resultssupporting

confidence: 93%

“…Additionally, at the concentration of 70:30 ( v / v , ACN:H 2 O), recoveries were varied slightly when the sample amount of honey increased from 0.3 g to 2 g. These results suggested that extraction yields were increased as the sample amount of honey increased, then reached a plateau when the concentration of honey was above the critical concentration. The high recovery values under the high concentration of sugars were also observed in sugaring-out extraction of protein and organic compounds by glucose [22,24,26,27]. This indicates that we can use a larger amount of honey sample under the high concentration of ACN to improve the sensitivity of the proposed matrix-induced sugaring-out procedure.…”

Section: Resultsmentioning

confidence: 80%

“…Sugaring-out assisted liquid-liquid extraction (SULLE) is a novel sample preparation method in which sugar is used to trigger phase separation [21,25,27]. ACN [21] and ethanol [30] have been reported to be applied as extraction solvents for sugaring-out.…”

Section: Resultsmentioning

confidence: 99%

“…This novel two-phase system shows the advantages of fast phase separation and is environmentally friendly and has found applications in protein purification [22], natural products extraction [23,24], and bioanalysis [25]. Recently, we reported a sugaring-out assisted liquid-liquid extraction (SULLE) method for the partition of 10-hydroxy-2-decenoic acid (10-HDA) in royal jelly [24] and multiple phenolic compounds in propolis [26] and a SULLE-based sample preparation method for the determination of bisphenol residues in royal jelly [27]. Honey is a natural sweetener composed of ca.…”

Section: Introductionmentioning

confidence: 99%

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Matrix-Induced Sugaring-Out: A Simple and Rapid Sample Preparation Method for the Determination of Neonicotinoid Pesticides in Honey

Chen

Zhang

et al. 2019

Molecules

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In the present work, we developed a simple and rapid sample preparation method for the determination of neonicotinoid pesticides in honey based on the matrix-induced sugaring-out. Since there is a high concentration of sugars in the honey matrix, the honey samples were mixed directly with acetonitrile (ACN)-water mixture to trigger the phase separation. Analytes were extracted into the upper ACN phase without additional phase separation agents and injected into the HPLC system for the analysis. Parameters of this matrix-induced sugaring-out method were systematically investigated. The optimal protocol involves 2 g honey mixed with 4 mL ACN-water mixture (v/v, 60:40). In addition, this simple sample preparation method was compared with two other ACN-water-based homogenous liquid-liquid extraction methods, including salting-out assisted liquid-liquid extraction and subzero-temperature assisted liquid-liquid extraction. The present method was fully validated, the obtained limits of detection (LODs) and limits of quantification (LOQs) were from 21 to 27 and 70 to 90 μg/kg, respectively. Average recoveries at three spiked levels were in the range of 91.49% to 97.73%. Precision expressed as relative standard deviations (RSDs) in the inter-day and intra-day analysis were all lower than 5%. Finally, the developed method was applied for the analysis of eight honey samples, results showed that none of the target neonicotinoid residues were detected.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Matrix-Induced Sugaring-Out: A Simple and Rapid Sample Preparation Method for the Determination of Neonicotinoid Pesticides in Honey

Chen

Zhang

et al. 2019

Molecules

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“…Organic solvents as a BPA extracting medium have been extensively studied and used over the years [ 30 , 31 , 32 ]. In recent years, liquid–liquid microextraction techniques have been widely used to extract BPA using different organic solvents, such as benzene [ 33 ], hexane [ 34 ], ethyl acetate [ 35 ] or ethanol [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

Determination of Very Low Concentration of Bisphenol A in Toys and Baby Pacifiers Using Dispersive Liquid–Liquid Microextraction by In Situ Ionic Liquid Formation and High-Performance Liquid Chromatography

Vicente-Martínez¹,

Caravaca²,

Soto-Meca³

2020

Pharmaceuticals

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Bisphenol A (BPA) is a chemical compound used in the manufacturing of plastics and resins whose presence in the body in low concentrations can cause serious health problems. Due to this, there is a growing interest in the scientific community to develop analytical methods that allow quantifying trace concentrations of BPA in different types of samples. The determination of this compound in toys made of plastics that can be manipulated by children leads to an extra concern, because it is possible for BPA to enter the body by introducing these toys into the mouth. This work presents a novel procedure to the quickly and easily quantification of trace levels of BPA in samples of toys and pacifiers according to the current demanding regulations. The determination of very low levels of BPA was carried out by ionic liquid dispersive liquid–liquid microextraction (IL-DLLME) followed by high-performance liquid chromatography (HPLC). The formation in situ of the ionic liquid (IL) 1-octyl-3-methylimidazolium bis((trifluoromethane)sulfonyl)imide ([C8MIm] [NTf2]), was achieved by mixing 1-octyl-3-methylimidazolium chloride ([C8MIm]Cl) and lithium bis(trifluoromethanesulfonyl)imide ([NTf2]Li) aqueous solutions, reaching an instant dispersion whose cloud of microdrops allows the total extraction of BPA in the IL from aqueous solutions. After centrifugation, BPA concentration in the sedimented phase was determined by HPLC. The optimal experimental conditions for the microextraction and determination of BPA in the IL were studied. The total extraction was achieved at pH 4, heating the sample at 30 °C for 5 min, using 100 µL of IL precursor volume, and spinning after the formation of dispersion at 3000 rpm for 10 min. The enrichment factor (EF) and detection limit (LOD) reached with the procedure were 299 and 0.19 µg L−1, respectively. The relative standard deviation for ten replications at the 0.5 µg L−1 level was 5.2%. Recovery studies showed a mean value for BPA recovery percentage in the samples of 99%. Additionally, a hybrid model was applied to characterize the extraction kinetics. This simple, low cost and fast method simplifies traditional microextraction techniques, representing an outstanding alternative.

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Matrix‐induced sugaring‐out liquid‐liquid microextraction coupled with high‐performance liquid chromatography for the determination of organophosphorus pesticides in fruit jams

Alkan

Çabuk

2022

Separation Science Plus

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A rapid and easy analytical method for the determination of organophosphorus pesticides (fenitrothion, phosalone, profenofos, and chlorpyrifos) in fruit jams was developed using sugaring-out liquid-liquid microextraction followed by high-performance liquid chromatography with ultraviolet detection. Acetonitrile was the only chemical used in the sample pretreatment step. The high sugar content of the sample matrix highly accelerated the extraction process as it simply triggered phase separation. The optimized sample treatment involved mixing 600 μl of ACN/water mixture (50/50%, v/v) with 1 g of jam sample, shaking for 0.5 min at 2500 rpm, and then centrifuging the sample for 5 min at 2500 × g. Under the optimal conditions, the extraction recoveries in the range of 81%-89% were achieved for the analytes. Good linearity in a range of 50-5000 μg/kg (r 2 ≥ 0.9992) and limits of detection less than 25 μg/kg were obtained. Recovery assays for spiked commercial jams made from cherry, strawberry, blackberry, apricot, and blueberry provided recoveries from 89% to 107%.

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Sugaring-Out Assisted Liquid-Liquid Extraction Combined with High-Performance Liquid Chromatography-Fluorescence Detection for the Determination of Bisphenol A and Bisphenol B in Royal Jelly

Cited by 19 publications

References 42 publications

Matrix-Induced Sugaring-Out: A Simple and Rapid Sample Preparation Method for the Determination of Neonicotinoid Pesticides in Honey

Matrix-Induced Sugaring-Out: A Simple and Rapid Sample Preparation Method for the Determination of Neonicotinoid Pesticides in Honey

Determination of Very Low Concentration of Bisphenol A in Toys and Baby Pacifiers Using Dispersive Liquid–Liquid Microextraction by In Situ Ionic Liquid Formation and High-Performance Liquid Chromatography

Matrix‐induced sugaring‐out liquid‐liquid microextraction coupled with high‐performance liquid chromatography for the determination of organophosphorus pesticides in fruit jams

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