Sugarcane Serine Peptidase Inhibitors, Serine Peptidases, and Clp Protease System Subunits Associated with Sugarcane Borer (Diatraea saccharalis) Herbivory and Wounding

Abstract

Sugarcane’s (Saccharum spp.) response to Diatraea saccharalis (F.) (Lepidoptera: (Crambidae) herbivory was investigated using a macroarray spotted with 248 sugarcane Expressed Sequence Tags (ESTs) encoding serine peptidase inhibitors, serine peptidases. and Clp protease system subunits. Our results showed that after nine hours of herbivory, 13 sugarcane genes were upregulated and nine were downregulated. Among the upregulated genes, nine were similar to serine peptidase inhibitors and four were similar to Bowman-Birk Inhibitors (BBIs). Phylogenetic analysis revealed that these sequences belong to a phylogenetic group of sugarcane BBIs that are potentially involved in plant defense against insect predation. The remaining four upregulated genes included serine peptidases and one homolog to the Arabidopsis AAA+ chaperone subunit ClpD, which is a member of the Clp protease system. Among the downregulated genes, five were homologous to serine peptidases and four were homologous to Arabidopsis Clp subunits (three homologous to Clp AAA+ chaperones and one to a ClpP-related ClpR subunit). Although the roles of serine peptidase inhibitors in plant defenses against herbivory have been extensively investigated, the roles of plant serine peptidases and the Clp protease system represent a new and underexplored field of study. The up- and downregulated D. saccharalis genes presented in this study may be candidate genes for the further investigation of the sugarcane response to herbivory.

Structural and Functional Characterization of PR-4 SUGARWINs From Sugarcaneand Their Role in Plant Defense

“…The plant defense system is under constant selective pressure to improve its response to pathogens and insect damage (Cui et al, 2002; Medeiros et al, 2016). Pathogen recognition by plants activates the host defense response, resulting in cell wall fortification via callose and lignin synthesis, the production of secondary metabolites such as phytoalexins that exhibit an antimicrobial effect, and the accumulation of pathogenesis-related proteins (PR proteins) (Pieterse and van Loon, 1999).…”

EXTRAÇÃO E CARACTERIZAÇÃO DAS TOXINAS PRESENTE EM LAGARTA Lonomia obliqua (LEPIDÓPTERA,SATURNIIDAE) COM ATIVIDADE ANTIBIOFILME

“…Diante disso há uma busca incessante por alternativas ao uso de compostos sintéticos e da crescente bioprospecção de extratos vegetais ricos em proteínas em busca de novas moléculas bioativas e eficazes no combate às pragas (KIM et al, 2010). Devido à sua natureza constitutiva, o papel dos inibidores pode ser ativado por um único gene, diante dissovários estudos sobre a expressão de IPs em plantas transgênicas vem sendo realizados em um esforço para aumentar a resistência das culturas contra esses insetos-pragas que desolam as plantações (MAHESWARAN et al, 2007;MEDEIROS et al, 2016;VELASQUES et al, 2017). frugiperda, a atividade inibitória se manteve dentro da faixa de inibição considerada para a prospecção a campo (50% -80%).…”

AVALIAÇÃO E SELEÇÃO IN SILICO DE PEPTÍDEOS ANTIMICROBIANOS BIOINSPIRADOS NA L-AMINOÁCIDO OXIDASE DA SERPENTE Bothrops jararacussu

“…Diante disso há uma busca incessante por alternativas ao uso de compostos sintéticos e da crescente bioprospecção de extratos vegetais ricos em proteínas em busca de novas moléculas bioativas e eficazes no combate às pragas (KIM et al, 2010). Devido à sua natureza constitutiva, o papel dos inibidores pode ser ativado por um único gene, diante dissovários estudos sobre a expressão de IPs em plantas transgênicas vem sendo realizados em um esforço para aumentar a resistência das culturas contra esses insetos-pragas que desolam as plantações (MAHESWARAN et al, 2007;MEDEIROS et al, 2016;VELASQUES et al, 2017).…”