Sugarcane inoculated with endophytic diazotrophic bacteria: effects on yield, biological nitrogen fixation and industrial characteristics

Abstract

The growing need for the use of clean energy has led society to seek alternatives to fossil fuels in order to less pollute the environment. Sugarcane has been known to produce enough biomass and to have associative microorganisms, such as plant growth-promoting bacteria (PgPB), which have the potential to improve the yield of crops. The objective of this study is to evaluate the efficiency and the isotopic dilution of 15 N of microbial inoculants containing endophytic diazotrophic bacteria, individually inoculated and in mixture, on productivity, technological quality and biological nitrogen fixation (BNF) of sugarcane variety RB92579. The experiment was conducted for three consecutive years, comprising different crop cycles, in a completely randomized block design with four replications. The evaluations were at 11 months after planting for plant-cane, and at 12 months after cutting for ratoon cane and second ratoon cane. The contribution of BNF by 15 N natural abundance technique to inoculated treatments ranged from 18% to 57.31%. This study showed that no strain promoted the improvement on yield and biological nitrogen fixation during the three cycles of sugarcane (plant-cane, ratoon cane and second ratoon cane). However, industrial characteristics of sugar cane can be affectd by PGPB inoculation.

Exploiting Biological Nitrogen Fixation: A Route Towards a Sustainable Agriculture

“…According to Serna-Cock et al [107], applying Azospirillum brasilense, Azotobacter chroccocum, and Trichoderma lignorum as biofertilizer in sugarcane plants (variety CC 934418) can replaces 60% of the nitrogen needed by this cultivar. Corroborating these results, Antunes et al [108] showed that inoculation with Herbaspirillum seropedicae, Pseudomonas sp., and Bacillus megaterium increase sugarcane (variety RB92579) yield from 18% to 57.31%. Although cereals benefit significantly from diazotrophs, most microbes are unlikely to fix nitrogen in the presence of high rate of chemical fertilizers.…”

Deciphering Microbial Community and Nitrogen Fixation in the Legume Rhizosphere

“…seropedicae, Pseudomonas sp., and B. megaterium, the sugar cane yield was >18% greater than that without inoculant …”

Complete genome analysis of sugarcane root associated endophytic diazotroph Pseudomonas aeruginosa DJ06 revealing versatile molecular mechanism involved in sugarcane development

“…The nitrogen contribution level in sugarcane reached 18–57.31%, verified by the 15 N natural abundance technique after inoculating nitrogen-fixing bacteria, including Herbaspirillum seropedicae IPA-CC9, Pseudomonas sp. IPA-CC33, and Bacillus megaterium IPA-CF6 (Antunes et al, 2019 ). The nitrogen uptake of sugarcane varieties, GT11 and B8, has significantly improved on inoculation of Enterobacter roggenkampii ED5 strain, and physiological enzymatic activities were also considerably changed (Guo et al, 2021 ).…”