Sugarcane growth promotion by Kosakonia sp. ICB117 an endophytic and diazotrophic bacterium

Kleingesinds, Carolina Krebs; Ferrara, Felipe Ibañez de Santi; Floh, Eny Iochevet Segal; Aidar, Marcos Pereira Marinho; Barbosa, Heloíza Ramos

doi:10.5897/ajmr2017.8738

Cited by 9 publications

(5 citation statements)

References 37 publications

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“…In addition to its use as a biostimulant, the capacity of the bacterial strains tested to synthesize IAA can be taken into account for the production of IAA, replacing the synthetic alternative and making the process more efficient and sustainable [ 55 ]. Some genera have already been reported as being able to produce IAA [ 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

Bacteria Associated with the Roots of Common Bean (Phaseolus vulgaris L.) at Different Development Stages: Diversity and Plant Growth Promotion

et al. 2022

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Current agricultural methodologies are vulnerable to erratic climate and are dependent on cost-intensive fertilization to ensure high yields. Sustainable practices should be pursued to ensure food security. Phaseolus vulgaris L. is one of the most produced legumes worldwide and may be an alternative to reduce the environmental impact of meat production as a reliable source of high-quality protein. Plant growth-promoting rhizobacteria (PGPR) are emerging as a sustainable option to increase agricultural production. To understand the dynamics between plants and microorganisms, the culturable microbiota of bean roots was isolated and identified at distinct stages of plant development (early and late vegetative growth, flowering, and pod) and root compartments (rhizoplane, endosphere, and nodules). Diversity and abundance of bacteria associated with root compartments differed throughout the plant life cycle. Bacterial plant growth promotion (PGP) and protection abilities (indole-3-acetic acid production, siderophore synthesis, and antifungal activity) were assessed and associated with plant phenology, demonstrating that among the bacteria associated with plant roots, several strains had an active role in the response to plant biological needs at each stage. Several strains stood out for their ability to display one or more PGP traits, being excellent candidates for efficient stage-specific biostimulants for application in precision agriculture.

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Section: Discussionmentioning

confidence: 99%

Bacteria Associated with the Roots of Common Bean (Phaseolus vulgaris L.) at Different Development Stages: Diversity and Plant Growth Promotion

et al. 2022

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“…Among the advantages of PSS system are quality and vigor control, greater homogeneity of seedlings and optimization of water and nutritional resources. Several studies have already described the beneficial effect of bacterial inoculants on sugarcane development (Kleingesinds et al 2018;Marcos et al 2016;Santos et al 2018;Silveira et al 2018), but the present study is the first to evaluate the benefit of plant-microorganism interactions with PGPB and AMF on sugarcane PSS focused in growth promoting and nutritional improvement. The evaluation of these beneficial microorganisms on sugarcane development can direct future studies on the inoculant recommendations for sugarcane PSS.…”

Section: Soil and Plant Nutrition Articlementioning

confidence: 99%

Sugarcane pre-sprouted seedlings produced with beneficial bacteria and arbuscular mycorrhizal fungi

et al. 2021

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The demand for the increased productivity of sugarcane crops has required changes in its production chain, such as the use of pre-sprouted seedlings (PSS) of sugarcane into the chain. In this system, the inoculation of beneficial microorganisms, such as plant growthpromoting bacteria (PGPB) and arbuscular mycorrhizal fungi (AMF), can improve seedling development. The objective was to evaluate the beneficial effect of PGPB and AMF inoculation on sugarcane PSS production system. Experiments were carried out in greenhouse using a commercial substrate with different levels of fertilization to evaluate the plant biomass and nutritional status. The bacteria strains are able to produce indole acetic acid and to amplify the nifH gene. The coinoculation of strains IAC-BeCa-095 with AMF (Glomus macrocarpum and Glomus etunicatum) improved the plant shoot biomass (25%) on the fertilized substrate. The strains IAC-BeCa-088 (Burkholderia caribensis), IAC-RBca5 (Pseudomonas sp.) and IAC-RBca10 (Bacillus sp.) without AMF and fertilization improved the shoot biomass by up to 35%. Coinoculation with strain IAC-BeCa-095 (Kosakonia radicincitans) and AMF improved the shoot and root biomass by up to 27 and 75%, respectively, in the conventionally fertilized substrate, demonstrating a synergistic effect of these microorganism consortia. The use of beneficial microorganisms may be a viable practice in the production of PSS sugarcane. Moreover, this study is the first to demonstrate the synergistic effect of endophytic bacteria (K. radicincitans) or rhizobacteria (Bacillus sp.) with AMF and Pseudomonas sp. or B. caribensis, without AMF inoculum on the production of sugarcane PSS to improve plant growth and plant nutrition.

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“…Bacteria of the genus Klebsiella belonging to the family Enterobacteriaceae stimulated plant growth via an array of direct and indirect mechanisms that are of increasing biotechnological interest due to their potential bio-inoculants for plant growth (Lin et al, 2019;Medina-Cordoba et al, 2021). Carolina et al (2018) reported that the N-containing molecules, amino acids, and mainly the excreted PAs could be derived when bacteria can fix nitrogen and release these compounds into either the rhizosphere or inside the plant. Genomic analysis of the endophytic nitrogen fixing bacteria DX120E revealed eight genes related to polyamine synthesis and 31 related to the transport and degradation.…”

Section: Discussionmentioning

confidence: 99%

“…Plates were incubated at 28°C in the dark for 1 day, and a dark red halo found beneath and around colonies indicated Put production by the decarboxylating isolates (El-Tarabily et al, 2021). Soluble polyamine concentrations in the culture medium were analyzed using the method described by Carolina et al (2018), adapted to bacterial cultures. The liquid culture supernatant was taken and tested for Spm, Spd, and Put using the ELISA Kit (Jiangsu MEIMIAN Co., China).…”

Section: Determination Of Pasmentioning

confidence: 99%

“…The contents of IAA, GA, and ACS in the inoculated sugarcane leaves indicated a gradual and significant increase compared to the un-inoculated sugarcane. Several authors attributed the increase in root development of plants inoculated with endophytic bacteria to the release of auxin by the bacteria (Carolina et al, 2018). Straub et al (2013) reported that endophytic bacteria H. frisingense affected the signaling of plant hormones, namely ethylene signaling, in root growth.…”

Section: B Amentioning

confidence: 99%

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Molecular mechanism of endophytic bacteria DX120E regulating polyamine metabolism and promoting plant growth in sugarcane

Qin,

Khan,

Yan

et al. 2024

Front. Plant Sci.

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IntroductionSugarcane endophytic nitrogen-fixing bacterium Klebsiella variícola DX120E displayed broad impact on growth, but the exact biological mechanism, especially polyamines (PAs) role, is still meager.MethodsTo reveal this relationship, the content of polyamine oxidase (PAO), PAs, reactive oxygen species (ROS)-scavenging antioxidative enzymes, phytohormones, 1-aminocyclopropane-1-carboxylic synthase (ACS), chlorophyll content, and biomass were determined in sugarcane incubated with the DX120E strain. In addition, expression levels of the genes associated with polyamine metabolism were measured by transcriptomic analysis.ResultsGenomic analysis of Klebsiella variícola DX120E revealed that 39 genes were involved in polyamine metabolism, transport, and the strain secrete PAs in vitro. Following a 7-day inoculation period, DX120E stimulated an increase in the polyamine oxidase (PAO) enzyme in sugarcane leaves, however, the overall PAs content was reduced. At 15 days, the levels of PAs, ROS-scavenging antioxidative enzymes, and phytohormones showed an upward trend, especially spermidine (Spd), putrescine (Put), catalase (CAT), auxin (IAA), gibberellin (GA), and ACS showed a significant up-regulation. The GO and KEGG enrichment analysis found a total of 73 differentially expressed genes, involving in the cell wall (9), stimulus response (13), peroxidase activity (33), hormone (14) and polyamine metabolism (4).DiscussionThis study demonstrated that endophytic nitrogen-fixing bacteria stimulated polyamine metabolism and phytohormones production in sugarcane plant tissues, resulting in enhanced growth. Dual RNA-seq analyses provided insight into the early-stage interaction between sugarcane seedlings and endophytic bacteria at the transcriptional level. It showed how diverse metabolic processes selectively use distinct molecules to complete the cell functions under present circumstances.

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Sugarcane growth promotion by Kosakonia sp. ICB117 an endophytic and diazotrophic bacterium

Cited by 9 publications

References 37 publications

Bacteria Associated with the Roots of Common Bean (Phaseolus vulgaris L.) at Different Development Stages: Diversity and Plant Growth Promotion

Bacteria Associated with the Roots of Common Bean (Phaseolus vulgaris L.) at Different Development Stages: Diversity and Plant Growth Promotion

Sugarcane pre-sprouted seedlings produced with beneficial bacteria and arbuscular mycorrhizal fungi

Molecular mechanism of endophytic bacteria DX120E regulating polyamine metabolism and promoting plant growth in sugarcane

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