Sugarcane Breeding, Germplasm Development and Supporting Genetic Research in Argentina

Ostengo, Santiago; Perera, María Francisca; Racedo, Josefina; González, Silvana Yacqueline Mamaní; Cornejo, Fernando Yáñez; Cuenya, María Inés

doi:10.1007/s12355-021-00999-z

Cited by 8 publications

(14 citation statements)

References 20 publications

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“…It has also contributed to sustained sugarcane production in other countries. In 2020, it was the leading sugarcane variety grown in two regions of Argentina (Tucumán [67%], and Jujuy and Salta [21%]) each with their own variety development program (Ostengo et al., 2022) (Figure 9). It is also actively being used for crossing in Argentina and is the parent of a recent release, TUC 06‐7 (Cuenya & Chavanne, 2020).…”

Section: Resultsmentioning

confidence: 99%

“…It is also actively being used for crossing in Argentina and is the parent of a recent release, TUC 06‐7 (Cuenya & Chavanne, 2020). After LCP 85‐384 was released in Tucumán in 2000, it expanded rapidly and occupied 45% of the acreage by 2005 (Ostengo et al., 2022), the same year it showed susceptibility to brown rust in Argentina (Cuenya et al., 2005; Ramallo et al., 2005) and 1 year after it showed susceptibility in Louisiana. Despite this, the acreage continued to increase, and it achieved its peak in 2013–2014 (Ostengo et al., 2022) (Figure 9).…”

Section: Resultsmentioning

confidence: 99%

“…After LCP 85‐384 was released in Tucumán in 2000, it expanded rapidly and occupied 45% of the acreage by 2005 (Ostengo et al., 2022), the same year it showed susceptibility to brown rust in Argentina (Cuenya et al., 2005; Ramallo et al., 2005) and 1 year after it showed susceptibility in Louisiana. Despite this, the acreage continued to increase, and it achieved its peak in 2013–2014 (Ostengo et al., 2022) (Figure 9). According to Dr. Kenneth Gravois, sugarcane specialist at the LSU AgCenter (Personal Communication, 2021), the rust infection on LCP 85‐384 in Argentina is inconsequential because it occurs towards the end of the growing season as opposed to in Louisiana where it occurs early in the growing cycle.…”

Section: Resultsmentioning

confidence: 99%

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The release of ‘LCP 85‐384’ and its contribution to the Louisiana sugar industry

Blanchard,

Taylor,

Rivette

et al. 2024

J of Plant Registrations

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Basic breeding efforts initiated at the USDA‐ARS in Houma, LA, in the late 1950s allowed Louisiana sugarcane breeders, once again, to cross with the wild germplasm of cultivated sugarcane, Sacharum spontaneum. ‘LCP 85‐384’ was the first success story from this effort and was quickly adopted by growers. It became clear that the cultivar would prove to be substantially impactful to the Louisiana sugar industry. Performing at unprecedented yields in ratoon crops, LCP 85‐384 changed how Louisiana growers would manage their crops. The variety proved to possess traits that fared well in the subtropical production zones of the state. Substantial stalk populations seemed to play a vital role in the necessary ratoon ability and consequent cold tolerance necessary for production in Louisiana. After significant disease pressure, the variety quickly declined in production acreage, but not in impact. It continued to provide sustained progress as a parent for years, contributing to most modern commercial cultivars in Louisiana. Today, LCP 85‐384 and its progeny are used in breeding and genetic research to usher in the next era of progress in the Louisiana sugar industry. Meanwhile, through cooperative efforts of breeding programs around the world, it is making its mark on the sugarcane industries of Argentina in much the same way as it did in Louisiana. In the future, it is expected that the progress made in the breeding program will continue by building upon the gains achieved from LCP 85‐384 and its progeny for the sustained success of the Louisiana sugarcane industry.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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The release of ‘LCP 85‐384’ and its contribution to the Louisiana sugar industry

Blanchard,

Taylor,

Rivette

et al. 2024

J of Plant Registrations

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“…In an attempt to commercially release a glyphosate-resistant variety in Argentina, an in depth study on transgenic sugarcane events of elite variety, RA 87-3, expressing C4 epsps gene from A. tumefaciens that confers glyphosate resistance was conducted. To evaluate the potential impact on different agricultural systems and food safety necessary for commercial deregulation of any transgenic event in Argentina, several health and environmental regulatory studies were carried out (Noguera et al, 2015;Perera et al, 2020;Enrique et al, 2021;Ostengo et al, 2021). These studies demonstrated the feasibility to produce transgenic varieties indistinguishable from their parental genotype regarding physiological, agronomical and industrial characters.…”

Section: Resistance To Herbicidesmentioning

confidence: 99%

Genetic Transformation of Sugarcane, Current Status and Future Prospects

et al. 2021

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Sugarcane (Saccharum spp.) is a tropical and sub-tropical, vegetative-propagated crop that contributes to approximately 80% of the sugar and 40% of the world’s biofuel production. Modern sugarcane cultivars are highly polyploid and aneuploid hybrids with extremely large genomes (>10 Gigabases), that have originated from artificial crosses between the two species, Saccharum officinarum and S. spontaneum. The genetic complexity and low fertility of sugarcane under natural growing conditions make traditional breeding improvement extremely laborious, costly and time-consuming. This, together with its vegetative propagation, which allows for stable transfer and multiplication of transgenes, make sugarcane a good candidate for crop improvement through genetic engineering. Genetic transformation has the potential to improve economically important properties in sugarcane as well as diversify sugarcane beyond traditional applications, such as sucrose production. Traits such as herbicide, disease and insect resistance, improved tolerance to cold, salt and drought and accumulation of sugar and biomass have been some of the areas of interest as far as the application of transgenic sugarcane is concerned. Although there have been much interest in developing transgenic sugarcane there are only three officially approved varieties for commercialization, all of them expressing insect-resistance and recently released in Brazil. Since the early 1990’s, different genetic transformation systems have been successfully developed in sugarcane, including electroporation, Agrobacterium tumefaciens and biobalistics. However, genetic transformation of sugarcane is a very laborious process, which relies heavily on intensive and sophisticated tissue culture and plant generation procedures that must be optimized for each new genotype to be transformed. Therefore, it remains a great technical challenge to develop an efficient transformation protocol for any sugarcane variety that has not been previously transformed. Additionally, once a transgenic event is obtained, molecular studies required for a commercial release by regulatory authorities, which include transgene insertion site, number of transgenes and gene expression levels, are all hindered by the genomic complexity and the lack of a complete sequenced reference genome for this crop. The objective of this review is to summarize current techniques and state of the art in sugarcane transformation and provide information on existing and future sugarcane improvement by genetic engineering.

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“…They also con rmed that S. o cinarum is the largest contributor, followed by S. barberi, and S. spontaneum. In addition, LCP 85-384, the most cultivated genotype (67.7%, according to Ostengo et al 2021) in Tucumán, has 3 sorghum genotypes in its genealogy (Sorghum bicolor cv Wiley, TADA and Rex) (Acevedo et al 2017). This complexity directly affects the rearrangement of chromosomes during meiosis and generates additional variation through the gain or loss of genetic material (Glyn et al 2004;Vieira et al 2018).…”

Section: Introductionmentioning

confidence: 99%

ddRADseq-mediated detection of genetic variants in sugarcane

et al. 2022

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Sugarcane (Saccharum sp.), a world-wide known feedstock for producing sugar, bioethanol, and energy, has an extremely complex genome due to its highly polyploid and aneuploid nature. A double digestion restriction site-associated DNA sequencing protocol (ddRADseq) was tested in four commercial sugarcane hybrids and one high-bre biotype for the detection and potential application of single nucleotide polymorphisms (SNPs) in genetic breeding. This genotyping approach compared two Illumina sequencing platforms and different ltering schemes, with and without a reference genome. A greater number of reads were obtained with Illumina platform Novaseq6000 than with Nextseq500, being this consistent with a greater number of SNPs determined with high accuracy. Additionally, more SNPs were found using a denovo pipeline compared to de refmap pipeline of the Stacks software. Longer read size, paired-end reads and 4M reads per individual represent the most e cient combination in recovering SNPs. The optimal combination of lter parameters varied depending on the different matrices created based on the different platforms. In both matrices, the highest number of SNPs localized in the longest chromosome 1, whereas the fewest landed in the shortest chromosomes 5 and 8. Multivariate comparisons of the SNPs matrices showed closer relationships among commercial hybrids than with the high-bre biotype. Functional analysis of the obtained SNPs, performed with the Variant Effect Predictor, demonstrated the appearance of variants throughout the sugarcane genome. The pipeline applied in this study can be exploited to identify useful molecular markers that can be used in sugarcane breeding programs to reduce selection cycles.

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Sugarcane Breeding, Germplasm Development and Supporting Genetic Research in Argentina

Cited by 8 publications

References 20 publications

The release of ‘LCP 85‐384’ and its contribution to the Louisiana sugar industry

The release of ‘LCP 85‐384’ and its contribution to the Louisiana sugar industry

Genetic Transformation of Sugarcane, Current Status and Future Prospects

ddRADseq-mediated detection of genetic variants in sugarcane

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