Sugar Transport &amp; Sugar Sensing In Grape

Agasse, Alice; Vignault, Ceݩline; Kappel, Christian; Conde, Carlos; Gerós, Hernâni; Delrot, Serge

doi:10.1007/978-90-481-2305-6_5

Cited by 36 publications

(36 citation statements)

References 214 publications

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“…[50], the unloading pathway shifts from symplastic to apoplastic at the onset of ripening, and is accompanied by a concominant increase in the expression and activity of cell wall invertases (cwINV). Proteomic data [31] have provided evidence for a switch between sucrose synthase (SuSy) and vacuolar acid invertase (vINV) before véraison , thus supporting a supposed functional mechanism between SuSy and INV for unloading sugars during grape berry development and ripening, respectively [84], as demonstrated in tomato [85-87]. In the current experiments, cwINV was not identified, but a vINV (Figure 6, ‘23’) was strongly up-regulated (14-fold) from 7-to-15 mm and protein levels were maintained during ripening.…”

Section: Resultsmentioning

confidence: 99%

iTRAQ-based protein profiling provides insights into the central metabolism changes driving grape berry development and ripening

et al. 2013

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BackgroundGrapevine (Vitis vinifera L.) is an economically important fruit crop. Quality-determining grape components such as sugars, acids, flavors, anthocyanins, tannins, etc., accumulate in the different grape berry development stages. Thus, correlating the proteomic profiles with the biochemical and physiological changes occurring in grape is of paramount importance to advance in our understanding of berry development and ripening processes.ResultsWe report the developmental analysis of Vitis vinifera cv. Muscat Hamburg berries at the protein level from fruit set to full ripening. An iTRAQ-based bottom-up proteomic approach followed by tandem mass spectrometry led to the identification and quantitation of 411 and 630 proteins in the green and ripening phases, respectively. Two key points in development relating to changes in protein level were detected: end of the first growth period (7 mm-to-15 mm) and onset of ripening (15 mm-to-V100, V100-to-110). A functional analysis was performed using the Blast2GO software based on the enrichment of GO terms during berry growth.ConclusionsThe study of the proteome contributes to decipher the biological processes and metabolic pathways involved in the development and quality traits of fruit and its derived products. These findings lie mainly in metabolism and storage of sugars and malate, energy-related pathways such as respiration, photosynthesis and fermentation, and the synthesis of polyphenolics as major secondary metabolites in grape berry. In addition, some key steps in carbohydrate and malate metabolism have been identified in this study, i.e., PFP-PFK or SuSy-INV switches among others, which may influence the final sugar and acid balance in ripe fruit. In conclusion, some proteins not reported to date have been detected to be deregulated in specific tissues and developmental stages, leading to formulate new hypotheses on the metabolic processes underlying grape berry development. These results open up new lines to decipher the processes controlling grape berry development and ripening.

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Section: Resultsmentioning

confidence: 99%

iTRAQ-based protein profiling provides insights into the central metabolism changes driving grape berry development and ripening

et al. 2013

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“…Several authors have reported similar effects on sugars and anthocyanins [35,36], but there is disagreement on the impact of thinning on titratable acidity, with reports suggesting that acidity is unaffected [7,37], slightly increased [1] or decreased by yield reduction [2,38]. In all cases, the effect on acidity seems to be related to the impact of cluster thinning on ripening, particularly the soluble solids content, because thinning reduces acidity only when the soluble solids concentration is strongly and positively affected ([38,39] and this study).…”

Section: Discussionmentioning

confidence: 99%

Increasing the source/sink ratio in Vitis vinifera (cv Sangiovese) induces extensive transcriptome reprogramming and modifies berry ripening

et al. 2011

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BackgroundCluster thinning is an agronomic practice in which a proportion of berry clusters are removed from the vine to increase the source/sink ratio and improve the quality of the remaining berries. Until now no transcriptomic data have been reported describing the mechanisms that underlie the agronomic and biochemical effects of thinning.ResultsWe profiled the transcriptome of Vitis vinifera cv. Sangiovese berries before and after thinning at veraison using a genome-wide microarray representing all grapevine genes listed in the latest V1 gene prediction. Thinning increased the source/sink ratio from 0.6 to 1.2 m2 leaf area per kg of berries and boosted the sugar and anthocyanin content at harvest. Extensive transcriptome remodeling was observed in thinned vines 2 weeks after thinning and at ripening. This included the enhanced modulation of genes that are normally regulated during berry development and the induction of a large set of genes that are not usually expressed.ConclusionCluster thinning has a profound effect on several important cellular processes and metabolic pathways including carbohydrate metabolism and the synthesis and transport of secondary products. The integrated agronomic, biochemical and transcriptomic data revealed that the positive impact of cluster thinning on final berry composition reflects a much more complex outcome than simply enhancing the normal ripening process.

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“…In plants, vacuoles contribute to long‐term and temporary storage of sugars (Agasse et al , Wingenter et al ). The composition of the vacuole and its volume depend on the coordinated activities of transporters and channels localized in the tonoplast (Reisen et al ).…”

Section: Introductionmentioning

confidence: 99%

The gene PbTMT4 from pear (Pyrus bretschneideri) mediates vacuolar sugar transport and strongly affects sugar accumulation in fruit

Cheng

Lü

et al. 2018

Physiologia Plantarum

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Tonoplast monosaccharide transporters (TMTs) play important roles in vacuolar sugar accumulation in plants. In this study, six TMT genes (PbTMT1-6) were identified in the Pyrus bretschneideri genome database, and their expression profiles were correlated with soluble sugar contents during the pear (P. bretschneideri cv. Ya Li) fruit development process. Subsequently, PbTMT4 was identified as a strong contributor to fructose, glucose and sucrose accumulation in fructescence of pears. Heterologous expression of PbTMT4, in the hexose transporter-deficient yeast strain EBY.VW4000, facilitated growth in media containing low levels of glucose, fructose, sucrose or sorbitol. In addition, PbTMT4-transformed tomato plants flowered and bore fruit significantly earlier than wild-type (WT) plants, and glucose and fructose levels in mature tomatoes were increased by about 32 and 21% compared with those in WT plants. However, no obvious alterations in sucrose content, plant height and weight per fruit were observed. Finally, subcellular localization experiments in transformed Arabidopsis plants showed that PbTMT4 is localized to tonoplast vesicles of protoplasts. These preliminary results suggest that PbTMT4 participates in vacuolar accumulation of sugars, and thus affects plant growth and development.

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Sugar Transport & Sugar Sensing In Grape

Cited by 36 publications

References 214 publications

iTRAQ-based protein profiling provides insights into the central metabolism changes driving grape berry development and ripening

iTRAQ-based protein profiling provides insights into the central metabolism changes driving grape berry development and ripening

Increasing the source/sink ratio in Vitis vinifera (cv Sangiovese) induces extensive transcriptome reprogramming and modifies berry ripening

The gene PbTMT4 from pear (Pyrus bretschneideri) mediates vacuolar sugar transport and strongly affects sugar accumulation in fruit

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