Sugar signals and molecular networks controlling plant growth

Smeekens, Sjef; Ma, Jingkun; Hanson, Johannes; Rolland, Filip

doi:10.1016/j.pbi.2009.12.002

Cited by 528 publications

(421 citation statements)

References 66 publications

Supporting

Mentioning

406

Contrasting

Unclassified

Order By: Relevance

“…T6P is an important regulatory molecule in plants with a large impact on metabolism, growth, and development (Eastmond et al, 2002;Schluepmann et al, 2003;Paul et al, 2008;Smeekens et al, 2010). We recently established a mechanistic basis for the signaling function of T6P in growing tissues through inhibition of SnRK1 of the SNF1/AMPK group of protein kinases (Zhang et al, 2009;Paul et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Wheat Grain Development Is Characterized by Remarkable Trehalose 6-Phosphate Accumulation Pregrain Filling: Tissue Distribution and Relationship to SNF1-Related Protein Kinase1 Activity

et al. 2011

View full text Add to dashboard Cite

Trehalose 6-phosphate (T6P) is a sugar signal that regulates metabolism, growth, and development and inhibits the central regulatory SNF1-related protein kinase1 (SnRK1; AKIN10/AKIN11). To better understand the mechanism in wheat (Triticum aestivum) grain, we analyze T6P content and SnRK1 activities. T6P levels changed 178-fold 1 to 45 d after anthesis (DAA), correlating with sucrose content. T6P ranged from 78 nmol g 21 fresh weight (FW) pregrain filling, around 100-fold higher than previously reported in plants, to 0.4 nmol g 21 FW during the desiccation stage. In contrast, maximum SnRK1 activity changed only 3-fold but was inhibited strongly by T6P in vitro. To assess SnRK1 activity in vivo, homologs of SnRK1 marker genes in the wheat transcriptome were identified using Wheat Estimated Transcript Server. SnRK1-induced and -repressed marker genes were expressed differently pregrain filling compared to grain filling consistent with changes in T6P. To investigate this further maternal and filial tissues were compared pre-(7 DAA) and during grain filling (17 DAA). Strikingly, in vitro SnRK1 activity was similar in all tissues in contrast to large changes in tissue distribution of T6P. At 7 DAA T6P was 49 to 119 nmol g 21 FW in filial and maternal tissues sufficient to inhibit SnRK1; at 17 DAA T6P accumulation was almost exclusively endospermal (43 nmol g 21 FW) with 0.6 to 0.8 nmol T6P g 21 FW in embryo and pericarp. The data show a correlation between T6P and sucrose overall that belies a marked effect of tissue type and developmental stage on T6P content, consistent with tissuespecific regulation of SnRK1 by T6P in wheat grain.

show abstract

Section: Discussionmentioning

confidence: 99%

Wheat Grain Development Is Characterized by Remarkable Trehalose 6-Phosphate Accumulation Pregrain Filling: Tissue Distribution and Relationship to SNF1-Related Protein Kinase1 Activity

et al. 2011

View full text Add to dashboard Cite

show abstract

“…SnRK1 controls metabolic enzymes directly by protein phosphorylation (Baena-González and Sheen, 2008). It also regulates greater than 1000 transcripts in response to carbohydrate availability, for example by adjusting bZIP transcription factor activity (Baena-González et al, 2007;Smeekens et al, 2010;Delatte et al, 2011;Matiolli et al, 2011;Mair et al, 2015). Both SnRK1-and hexokinasemediated sugar signaling involve specific sugars functioning as signaling molecules that provide cellular information concerning sugar availability.…”

mentioning

confidence: 99%

The Energy-Signaling Hub SnRK1 Is Important for Sucrose-Induced Hypocotyl Elongation

et al. 2017

View full text Add to dashboard Cite

Emerging seedlings respond to environmental conditions such as light and temperature to optimize their establishment. Seedlings grow initially through elongation of the hypocotyl, which is regulated by signaling pathways that integrate environmental information to regulate seedling development. The hypocotyls of Arabidopsis (Arabidopsis thaliana) also elongate in response to sucrose. Here, we investigated the role of cellular sugar-sensing mechanisms in the elongation of hypocotyls in response to Suc. We focused upon the role of SnRK1, which is a sugar-signaling hub that regulates metabolism and transcription in response to cellular energy status. We also investigated the role of TPS1, which synthesizes the signaling sugar trehalose-6-P that is proposed to regulate SnRK1 activity. Under light/dark cycles, we found that Suc-induced hypocotyl elongation did not occur in tps1 mutants and overexpressors of KIN10 (AKIN10/SnRK1.1), a catalytic subunit of SnRK1. We demonstrate that the magnitude of Suc-induced hypocotyl elongation depends on the day length and light intensity. We identified roles for auxin and gibberellin signaling in Suc-induced hypocotyl elongation under short photoperiods. We found that Suc-induced hypocotyl elongation under light/dark cycles does not involve another proposed sugar sensor, HEXOKINASE1, or the circadian oscillator. Our study identifies novel roles for KIN10 and TPS1 in mediating a signal that underlies Suc-induced hypocotyl elongation in light/dark cycles.

show abstract

“…As sessile organisms, land plants endure continual environmental fluctuations that may abruptly deplete their energy stores. To survive such challenges, plant SnRK1s modulate transcriptional, metabolic, and developmental processes (Hey et al, 2010;Smeekens et al, 2010). In Arabidopsis (Arabidopsis thaliana), SnRK1s KIN10 and KIN11 manage energy-depleting conditions through the control of stress-responsive gene expression and stress hormone abscisic acid signaling, which also modulates plant susceptibility to pathogens (Baena-González et al, 2007;Jossier et al, 2009).…”

mentioning

confidence: 99%

Regulatory Functions of SnRK1 in Stress-Responsive Gene Expression and in Plant Growth and Development

et al. 2012

View full text Add to dashboard Cite

Sucrose-nonfermentation1-related protein kinase1 (SnRK1) is an evolutionarily conserved energy sensor protein that regulates gene expression in response to energy depletion in plants. Efforts to elucidate the functions and mechanisms of this protein kinase are hampered, however, by inherent growth defects of snrk1-null mutant plants. To overcome these limitations and study SnRK1 functions in vivo, we applied a method combining transient expression in leaf mesophyll protoplasts and stable expression in transgenic plants. We found that both rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana) SnRK1 activities critically influence stress-inducible gene expression and the induction of stress tolerance. Genetic, molecular, and chromatin immunoprecipitation analyses further revealed that the nuclear SnRK1 modulated target gene transcription in a submergencedependent manner. From early seedling development through late senescence, SnRK1 activities appeared to modulate developmental processes in the plants. Our findings offer insight into the regulatory functions of plant SnRK1 in stressresponsive gene regulation and in plant growth and development throughout the life cycle.

show abstract

Sugar signals and molecular networks controlling plant growth

Cited by 528 publications

References 66 publications

Wheat Grain Development Is Characterized by Remarkable Trehalose 6-Phosphate Accumulation Pregrain Filling: Tissue Distribution and Relationship to SNF1-Related Protein Kinase1 Activity

Wheat Grain Development Is Characterized by Remarkable Trehalose 6-Phosphate Accumulation Pregrain Filling: Tissue Distribution and Relationship to SNF1-Related Protein Kinase1 Activity

The Energy-Signaling Hub SnRK1 Is Important for Sucrose-Induced Hypocotyl Elongation

Regulatory Functions of SnRK1 in Stress-Responsive Gene Expression and in Plant Growth and Development

Contact Info

Product

Resources

About