Sugar sensing in higher plants

Jang, Jyan Chyun; Sheen, Jen

doi:10.1016/s1360-1385(97)89545-3

Cited by 410 publications

(395 citation statements)

References 103 publications

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“…This reduction in Rubisco activity was the result of a similar loss of Rubisco protein (Krapp etaL, 1993;Van Oosten and Besford, 1995) which was concomitant with a reduction in RBCS transcript (Criqui etaL, 1992;Krapp et aL, 1993;Van Oosten and Besford, 1995) and a repression of the transcriptional activity of a RBCS promoter (Krapp et aL, 1993;Sheen, 1989). The repression of RBCSand other genes by carbohydrates suggests a common mechanism of control of nuclear genes coding for photosynthetic proteins (Jang and Sheen, 1994;Jang etaL, 1997;Sheen, 1989Sheen, , 1994. Like RBCS and CAB genes, it was recently demonstrated that promoter activity of the Arabidopsis PC gene was repressed by sugars in Arabidopsis seedlings (Dijkwel et aL, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…Here, the use of a genetic approach has been tremendously important for the identification of sensing processes and for cloning the genes involved. Interestingly, in yeast, hexokinase was found to be important for carbohydrate sensing and recent results provide evidence that hexokinase has a similar function in plants (Graham etal., 1994;Jang and Sheen, 1994;Jang etal., 1997). In this paper, we describe the analysis of three sucroseuncoupled Arabidopsis mutants defective in sugar sensing.…”

Section: Discussionmentioning

confidence: 99%

“…This glucose analog is phosphorylated by hexokinase and is partially metabolized (Guy and Reinhold, 1974). At a relatively low concentration, it mimics the effect of glucose on photosynthetic gene expression (Sheen, 1989;Jang and Sheen, 1994) and on genes involved in the glyoxylate cycle (Graham etaL, 1994). The advantage of using 2DG is that there is no contamination by micro-organisms for up to two weeks, whereas the use of sucrose and glucose rapidly results in massive contamination.…”

Section: Reversibility Of the Effect Of 2dg Treatment On Gas Exchangementioning

confidence: 99%

“…Results obtained using a biochemical approach suggested that plant hexokinase is involved in sugar-sensing, leading to repression of photosynthetic and glyoxylateassociated genes (Graham et al, 1994;Jang and Sheen, 1994;Jang et aL, 1997). After the phosphorylation by hexokinase, no further metabolism of glucose was required to induce repression (Graham etal., 1994;Jang and Sheen, 1994).…”

Section: Introductionmentioning

confidence: 99%

“…After the phosphorylation by hexokinase, no further metabolism of glucose was required to induce repression (Graham etal., 1994;Jang and Sheen, 1994). 2-deoxy-g-glucose (2DG) which is not rapidly metabolized in plants could mimic the control by glucose of gene expression (Graham etal., 1994;Jang and Sheen, 1994;Sheen, 1989).…”

Section: Introductionmentioning

confidence: 99%

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An Arabidopsis mutant showing reduced feedback inhibition of photosynthesis

et al. 1997

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SummaryMany plant genes are responsive to sugars but the mechanisms used by plants to sense sugars are unknown, A genetic approach has been used in Arabidopsis to identify genes involved in perception and transduction of sugar signals. For this purpose, an in vivo reporter system was established consisting of the light-and sugar-regulated plastocyanin promoter, fused to the luciferase coding sequence (PC-LUC construct). At the seedling stage, expression of the PC-LUC gene is repressed by sucrose, and a number of sucrose-uncoupled (sun) mutants were selected in which sucrose is unable to repress the activity of the PC promoter. Three mutants have been characterized in more detail. The sugar analog 2-deoxy-D-glucose (2DG) was used to repress whole plant photosynthesis, PC-LUC gene expression and total ribulose-l,5-bisphosphate activity. It was found that the sun6 mutation makes plants unresponsive to these 2DG-induced effects. Moreover, unlike wild-type plants, sun6 mutants are insensitive to elevated levels of glucose in the growth medium, These findings suggest that the SUN6gene is active in a hexoseactivated signal transduction pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Reversibility Of the Effect Of 2dg Treatment On Gas Exchangementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

An Arabidopsis mutant showing reduced feedback inhibition of photosynthesis

et al. 1997

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Increasing Rice Productivity and Yield by Manipulation of Starch Synthesis

Okita

Sun

Sakulringharoj

et al. 2007

Novartis Foundation Symposia

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Metabolomics: Integrating the Metabolome and the Proteome for Systems Biology

Weckwerth

2018

Annual Plant Reviews Online

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The term ‘metabolome’ was coined in analogy to ‘genome’ and ‘proteome’ and relates to the metabolism. The metabolome contains all the characteristic metabolic activities of a cell or tissue and can be described on the basis of conversion rates, enzyme activities and intermediates, the set of metabolites. In order to study a cell, tissue or an organism in its entirety it is not sufficient to merely consider its metabolome alone. Only the interactions and synergisms between the transcriptome, the proteome and finally the metabolome will furnish the key to a functioning living system. This approach does not only promise to provide an improved picture of the metabolic processes and the interaction. In this way, we also hope to understand the function of hitherto unknown genes representing a large proportion of all so far published genomes. Finally, computer models for making predictions of the metabolic network and their iterative linkage to experimental data via the interaction of proteins and metabolic processes may contribute to studying fundamental mechanisms of metabolism.

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Sugar sensing in higher plants

Cited by 410 publications

References 103 publications

An Arabidopsis mutant showing reduced feedback inhibition of photosynthesis

An Arabidopsis mutant showing reduced feedback inhibition of photosynthesis

Increasing Rice Productivity and Yield by Manipulation of Starch Synthesis

Metabolomics: Integrating the Metabolome and the Proteome for Systems Biology

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