Sugar recoveries from wheat straw following treatments with the fungus Irpex lacteus

Characterization of a Novel Dye-Decolorizing Peroxidase (DyP)-Type Enzyme from Irpex lacteus and Its Application in Enzymatic Hydrolysis of Wheat Straw

“…The detection of Mn-independent peroxidase in I. lacteus growing on wheat straw has been recently described (9). With the aim of producing and purifying it, peroxidase production was followed in a liquid medium (CSS) known to produce high I. lacteus biomass in comparison with other media (9). According to the peroxidase production profile found in the present study, it can be suggested that the fungus starts producing MnPs when glucose is almost consumed.…”

“…lacteus and its extracellular oxidative enzymatic system offer a great potential for different biotechnological applications. The detection of Mn-independent peroxidase in I. lacteus growing on wheat straw has been recently described (9). With the aim of producing and purifying it, peroxidase production was followed in a liquid medium (CSS) known to produce high I. lacteus biomass in comparison with other media (9).…”

“…The effectiveness of different enzyme mixtures in wheat straw hydrolysis was checked by measuring the cellulose and hemicellulose digestibility yields. Prior to the enzymatic hydrolysis, wheat straw was either left untreated or subjected to biopretreatment with I. lacteus for 21 days under basal conditions, either with or without a mild alkali treatment (0.1% sodium hydroxide), as previously reported (9). Hydrolysis of the different substrates was performed in duplicate at 5% (wt/vol) in 100 mM sodium citrate buffer (pH 4.8) at 50°C and 165 rpm for 60 h using the following enzymatic mixtures: (i) commercial cellulases, such as Celluclast (15 U of global cellulase) and NS50010 (15 U of ␤-glucosidase), and xylanases, such as NS50013 (30 U of global xylanase) and NS50030 (30 U of ␤-xylosidase), all provided by Novozymes; (ii) the previous commercial cocktails plus the enzymatic crude extract of I. lacteus containing 30 U of DyP; (iii) only the enzymatic crude extract of I. lacteus (30 U of DyP); and (iv) the previous commercial cocktails plus the purified DyP (30 U of DyP which corresponded to 150 g of protein).…”

“…After the stability of the enzyme against H 2 O 2 was proved, an aliquot of 0.8 mM H 2 O 2 was added to all samples at 0, 24, and 48 h. Controls consisted of substrates plus buffer without any enzyme supplementation and were incubated under the same conditions. Substrate characterization (percentage of cellulose and hemicellulose before and after biopretreatment), fermentable sugar release quantification, and digestibility estimations were performed as detailed by Salvachúa et al (9). and ABTS, which corresponded to MnP, was detected at 7 days.…”

Solid State Fermentation: Comprehensive Tool for Utilization of Lignocellulosic through Biotechnology

“…Biofertilizers produced from agro-waste using SSF are found to be more economical in its production and most potent in improving the soil quality and significantly enhance the crop yield. Different wastes from the fruits like, banana, watermelon, papaya, pineapple, citrus orange [120] [121] [122] can be very good substrates for the production of biofertilizer, which are applied to the vegetable plantation [45]. Health consciousness in human beings navigated them towards organic agricultural products and this rapid expansion demanded for inexpensive phosphate source which imposed farmers to apply insoluble rock phosphate, the direct source of soil pollution and eutrophication.…”