Sugar effects on early seedling development in Arabidopsis

Rognoni, Sara; Teng, Sheng; Arru, Laura; Smeekens, Sjef; Perata, Pierdomenico

doi:10.1007/s10725-007-9193-z

Cited by 40 publications

(37 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The analysis of several sugar-sensing mutants reveals that the developmental arrest triggered by sugars is largely independent of the osmotic effects. Furthermore, recent studies have also highlighted that the sugar-sensing processes are uncoupled from the role of sugars as nutrients (Rognoni et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

Wojtania¹,

Wegrzynowicz-Lesiak²,

Dziurka³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

View full text Add to dashboard Cite

The aim of the study was to determine the effect of exogenous sucrose and cytokinin on ethylene production and responsiveness in relation to the shoot formation of Pelargonium × hortorum 'Bergpalais' in vitro. Increasing the concentration of sucrose from 15 to 40 g L -1 in medium containing meta-topolin (mT) resulted in a two-fold decrease in the number of shoots and leaves as well as a reduction in ethylene production. The addition of ethylene synthesis inhibitor (AVG) to mT-medium significantly reduced the ethylene production and the shoot growth, but it had no significant influence on the shoot formation. The mT-induced shoot formation was, however, significantly reduced in the presence of ethylene action inhibitor (AgNO 3 ), in a manner dependent on sucrose levels. At the end of the subculture period, increased sucrose concentrations (15-40 g L -1) in the presence of mT and AgNO 3 resulted in a 3.7-fold increase in ethylene emission. At the same time, the supply of sucrose caused a 2.8-fold increase in the level of endogenous abscisic acid (ABA). Our results may suggest that the inhibitory effect of high sucrose concentration (30 and 40 g L -1 ) may depend on its influence on ethylene sensitivity. It also suggests that sucrose-regulation of the shoot formation of Pelargonium in vitro is mediated by ABA.K Ke ey y w wo or rd ds s: : Ethylene inhibitors, endogenous ABA, geranium, meta-topolin, sucrose concentration, shoot multiplication.

show abstract

Section: Discussionmentioning

confidence: 99%

Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

Wojtania¹,

Wegrzynowicz-Lesiak²,

Dziurka³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

View full text Add to dashboard Cite

show abstract

“…It has been shown that this process is closely associated with plant hormone biosynthesis and signalling, in particular with that of ABA (for review see Finkelstein and Gibson, 2001; Gazzarini and McCourt, 2001; León and Sheen, 2003;Rook et al, 2006;Dekkers and Smeekens, 2007;Rognoni et al, 2007). Mutants that were insensitive to the inhibiting effect of glucose and sucrose on early seedling development were isolated by several groups and identified ABA deficient mutants (i.e.…”

Section: The Genetic Control Of Seed Storage Compounds Seed Deteriormentioning

confidence: 99%

Seed Dormancy and Germination

Bentsink

Koornneef

2008

The Arabidopsis Book

322

271

View full text Add to dashboard Cite

Seed dormancy allows seeds to overcome periods that are unfavourable for seedling established and is therefore important for plant ecology and agriculture. Several processes are known to be involved in the induction of dormancy and in the switch from the dormant to the germinating state. The role of plant hormones, the different tissues and genes involved, including newly identified genes in dormancy and germination are described in this chapter, as well as the use transcriptome, proteome and metabolome analyses to study these mechanistically not well understood processes. of 18 The Arabidopsis BookFinch-Savage and Leubner-Metzger, 2006). Dormancy in Arabidopsis should be described as physiologically non-deep, meaning that embryos released from surrounding structures grow normally, and that dormancy is lost through moist chilling (stratification) or after-ripening (Baskin and Baskin, 2004). However, in addition to the testa and endosperm layer surrounding the embryo, the growth potential of the embryo is also important to overcome the constraint of these structures and thereby affects the dormancy state of a seed (Kucera et al., 2006). Since dormancy is regulated at different developmental phases, in interaction with environmental factors, it is difficult to detect when the genetic and physiological differences are established. This difficulty arises because all dormancy assays are based on seed germination, which is the result of the balance between the degree of dormancy and the capacity of the embryo to overcome dormancy. Mechanistically one can distinguish factors that influence dormancy and germination on the basis of their effect on germination, being either inhibiting or promoting. Mutants that germinate better or faster can represent genes that promote dormancy or those that repress germination. A further distinction can be made by defining the timing and site of action of these factors (during maturation or during imbibition of the seeds, in the embryo, the endosperm or in the testa). The interaction between these factors and the large effect of the environment, both during seed development and during imbibition, make seed dormancy a very complex trait.By definition, germination incorporates those events that commence with the uptake of water by the quiescent dry seed and terminates with the elongation of the embryonic axis (Bewley and Black, 1994). Water uptake by a seed is triphasic; phase I rapid initial uptake; phase II plateau phase and in phase III further increase of water uptake, however, only when germination occurs (Schopfer and Plachy, 1984; Bewley, 1997;Manz et al., 2005). The first signs of germination are the resumption of essential processes, including transcription, translation and DNA repair followed by cell-elongation and eventually at the time of radicle protrusion, resumption of cell division (Barroco et al., 2005;Masubelele et al., 2005). Physically germination is a two-stage process, where testa rupture is followed by endosperm rupture. Following rupture of the micropylar endosper...

show abstract

“…Because mutants in sugar sensing often exhibit seed germination phenotypes Gibson, 2005;Price et al, 2003;Rognoni et al, 2007), we also evaluated the sugar and ABA sensitivity of germination in the xlg triple mutant (Figure 7b). At low sugar concentrations (1% or 3%), germination of the xlg triple mutant was statistically identical to wild type with all sugars tested, including sucrose.…”

Section: Xlgs Are Negative Regulators Of Primary Root Lengthmentioning

confidence: 99%

Arabidopsis extra‐large G proteins (XLGs) regulate root morphogenesis

Ding

Pandey

Assmann³

2007

The Plant Journal

112

130

View full text Add to dashboard Cite

SummaryAs in mammalian systems, heterotrimeric G proteins, composed of a, b and c subunits, are present in plants and are involved in the regulation of development and cell signaling. Besides the sole prototypical G protein a subunit gene, GPA1, the Arabidopsis thaliana genome has three extra-large GTP-binding protein (XLG)-encoding genes: XLG1 (At2g23460), XLG2 (At4g34390) and XLG3 (At1g31930). The C-termini of the XLGs are Ga domains that are homologous to GPA1, whereas their N-termini each contain a cysteine-rich region and a putative nuclear localization signal (NLS). GFP fusions with each XLG confirmed nuclear localization. All three XLG genes are expressed in essentially all plant organs, with strong expression in vascular tissues, primary root meristems and lateral root primordia. Analysis of single, double and triple T-DNA insertional mutants of the XLG genes revealed redundancy in XLG function. Dark-grown xlg1-1 xlg2-1 xlg3-1 triple mutant plants showed markedly increased primary root length compared with wild-type plants. This phenotype was not observed in dark-grown xlg single mutants, and was suppressed upon complementation of the xlg triple mutant with each XLG. Root cell sizes of the xlg triple mutant and root morphology were highly similar to those of wild-type roots, suggesting that XLGs may regulate cell proliferation. Dark-grown roots of the xlg triple mutants also showed altered sensitivity to sugars, ABA hyposensitivity and ethylene hypersensitivity, whereas seed germination in xlg triple mutants was hypersensitive to osmotic stress and ABA. As plantspecific proteins, regulatory mechanisms of XLGs may differ from those of conventional Gas.

show abstract

Sugar effects on early seedling development in Arabidopsis

Cited by 40 publications

References 91 publications

Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

Sucrose and Cytokinin Interactions in Relation to Ethylene and Abscisic Acid Production in the Regulation of Morphogenesis in Pelargonium × hortorum L.H. Bailey In Vitro

Seed Dormancy and Germination

Arabidopsis extra‐large G proteins (XLGs) regulate root morphogenesis

Contact Info

Product

Resources

About