Sugar-Dependent Expression of the <i>CHS-A</i> Gene for Chalcone Synthase from Petunia in Transgenic <i>Arabidopsis</i>

Tsukaya, Hirokazu; Ohshima, Toshiyuki; Naito, Shuichi; Chino, Mitsuo; Komeda, Yoshibumi

doi:10.1104/pp.97.4.1414

Cited by 252 publications

(192 citation statements)

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“…Elevated sugar levels cause stunted growth, reduced photosynthesis, enhanced anthocyanin accumulation, and curled, chlorotic, and necrotic leaves (Casper et al, 1986;von Schaewen et al, 1990;Dickinson et al, 1991;Sonnewald et al, 1991Tsukaya et al, 1991;Huber and Hanson, 1992). In addition, environmental factors, such as elevated C02 (Stitt, 1991;Stitt et al, 1991;van Oosten et al, 1994;Nie To whom correspondence should be addressed at Department of Molecular Biology, Massachusetts General Hospital, Wellman 11 17, Boston, MA 021 14.…”

Section: Introductionmentioning

confidence: 99%

Hexokinase as a sugar sensor in higher plants.

et al. 1997

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Section: Introductionmentioning

confidence: 99%

Hexokinase as a sugar sensor in higher plants.

et al. 1997

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“…Examples of sugar-induced genes involved in metabolism are maize invertase or sucrose synthase genes (Koch, 1996), an Arabidopsis nitrate reductase (NR) gene (Cheng et al, 1992) and a subunit of ADP-glucose pyrophosphorylase in potato and tomato plants (M~iller-R6ber et al, 1990;Van Oosten etal., 1994). In addition, genes that encode proteins which have a presumed function in plant defence such as the Arabidopsis chalcone synthase (CHS, Tsukaya et al, 1991), a potato protease inhibitor (PIN2, Pena-Cort~z etal., 1992) and other PR genes (Herbers etal., 1996) were induced by sucrose. Genes encoding storage proteins are also positively regulated by sucrose (VSPB, Sadka etal., 1994).…”

Section: Introductionmentioning

confidence: 99%

An Arabidopsis mutant showing reduced feedback inhibition of photosynthesis

et al. 1997

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SummaryMany plant genes are responsive to sugars but the mechanisms used by plants to sense sugars are unknown, A genetic approach has been used in Arabidopsis to identify genes involved in perception and transduction of sugar signals. For this purpose, an in vivo reporter system was established consisting of the light-and sugar-regulated plastocyanin promoter, fused to the luciferase coding sequence (PC-LUC construct). At the seedling stage, expression of the PC-LUC gene is repressed by sucrose, and a number of sucrose-uncoupled (sun) mutants were selected in which sucrose is unable to repress the activity of the PC promoter. Three mutants have been characterized in more detail. The sugar analog 2-deoxy-D-glucose (2DG) was used to repress whole plant photosynthesis, PC-LUC gene expression and total ribulose-l,5-bisphosphate activity. It was found that the sun6 mutation makes plants unresponsive to these 2DG-induced effects. Moreover, unlike wild-type plants, sun6 mutants are insensitive to elevated levels of glucose in the growth medium, These findings suggest that the SUN6gene is active in a hexoseactivated signal transduction pathway.

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“…Because CHS gene expression is regulated by various stimuli, including UV light (Tsukaya et al, 1991;Fuglevand et al, 1996;Ni et al, 1996;van Eldik et al, 1997;Hartmann et al, 1998;Kubasek et al, 1998), it was important to investigate if the constitutive expression of CHS in uvt1 was due to a defect in the UV-mediated regulation of the CHS gene. The data in Figure 9 (lane 4) show that CHS gene mRNA levels are significantly elevated after UVB exposure and, therefore, that the UVB-mediated regulation of CHS gene expression is still operating in uvt1.…”

Section: Chalcone Synthase (Chs) Mrna Levels In Uvt1 Are Constitutivementioning

confidence: 99%

An Arabidopsis Mutant Tolerant to Lethal Ultraviolet-B Levels Shows Constitutively Elevated Accumulation of Flavonoids and Other Phenolics

2001

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The isolation and characterization of mutants hypersensitive to ultraviolet (UV) radiation has been a powerful tool to learn about the mechanisms that protect plants against UV-induced damage. To increase our understanding of the various mechanisms of defense against UVB radiation, we searched for mutations that would increase the level of tolerance of Arabidopsis plants to UV radiation. We describe a single gene dominant mutation (uvt1) that leads to a remarkable tolerance to UVB radiation conditions that would kill wild-type plants. Pigment analyses show a constitutive increase in accumulation of UV-absorbing compounds in uvt1 that increases the capacity of the leaves to block UVB radiation and therefore is likely to be responsible for the elevated resistance of this mutant to UVB radiation. These increases in absorption in the UV region are due, at least in part, to increases in flavonoid and sinapate accumulation. Expression of chalcone synthase (CHS) mRNA was shown to be constitutively elevated in uvt1 plants, suggesting that the increases in absorption may be a consequence of changes in gene expression. Expression of CHS in uvt1 was shown to be still inducible by UV, indicating that the uvt1 lesion may not affect the UV-mediated regulation of CHS gene expression. Our data support an important role for UV screens in the overall protection of plants to UVB radiation. The uvt1 mutant could prove to be an important tool to elucidate further the exact role of UV-absorbing pigments in UV protection as well as the relative contribution of other mechanisms to the overall tolerance of plants to UV radiation.

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Sugar-Dependent Expression of the CHS-A Gene for Chalcone Synthase from Petunia in Transgenic Arabidopsis

Abstract: Transgenic Arabidopsis thaliana plants were constructed by introduction of a fusion of the gene for #-glucuronidase (GUS) to

Cited by 252 publications

References 31 publications

Hexokinase as a sugar sensor in higher plants.

Hexokinase as a sugar sensor in higher plants.

An Arabidopsis mutant showing reduced feedback inhibition of photosynthesis

An Arabidopsis Mutant Tolerant to Lethal Ultraviolet-B Levels Shows Constitutively Elevated Accumulation of Flavonoids and Other Phenolics

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