Sugar compositional determination of polysaccharides from Dunaliella salina by modified RP-HPLC method of precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone

Dai, Jun; Wu, Yan; Chen, Shangwei; Zhu, Shuguang; Yin, Huijuan; Wang, Min; Tang, Jian

doi:10.1016/j.carbpol.2010.05.029

Cited by 301 publications

(116 citation statements)

References 22 publications

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“…The protein, uronic acid and sulfate of polysaccharide were assayed by folin phenol (Lowry et al, 1951), sulfuric acid-carbazole (Bitter and Muir, 1962), and barium sulfate turbidimetry (Dai et al, 2010), respectively. The yield of polysaccharide was determined by the phenol-sulphuric acid method (Dubois et al, 1956) and calculated as follows:…”

Section: Methodsmentioning

confidence: 99%

Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities

Wan

Yang

Cai

et al. 2015

J. Ocean Univ. China

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In the present study, ultrasonic extraction technique (UET) is used to improve the yield of polysaccharides from Laminaria japonica (LJPs). And their antioxidative as well as glycosidase inhibitory activities are investigated. Box-Behnken design (BBD) combined with response surface methodology (RSM) is applied to optimize ultrasonic extraction for polysaccharides. The optimized conditions are obtained as extraction time at 54 min, ultrasonic power at 1050 W, extraction temperature at 80℃ and ratio of material to solvent at 1:50 (g mL −1 ). Under these optimal ultrasonic extraction conditions, an actual experimental yield (5.75% ± 0.3%) is close to the predicted result (5.67%) with no significant difference (P > 0.05). Vitro antioxidative and glycosidase inhibitory activities tests indicate that the crude polysaccharides (LJP) and two major ethanol precipitated fractions (LJP1 and LJP2) are in a concentration-dependent manner. LJP2 (30%60% ethanol precipitated polysaccharides) possesses the strongest α-glucosidase inhibitory activity and moderate scavenging activity against hydroxyl radicals (66.09% ± 2.19%, 3.0 mg mL −1 ). Also, the inhibitory activity against α-glucosidase (59.08% ± 3.79%, 5.0 mg mL −1 ) is close to that of acarbose (63.99% ± 3.27%, 5.0 mg mL −1 ). LJP1 (30% ethanol precipitated polysaccharides) exhibits the strongest scavenging activity against hydroxyl radicals (99.80% ± 0.00%, 3.0 mg mL −1 ) and moderate α-glucosidase inhibitory activity (47.76% ± 1.92%, 5.0 mg mL −1 ). LJP shows the most remarkable DPPH scavenging activity (66.20% ± 0.11%, 5.0 mg mL −1 ) but weakest α-glucosidase inhibitory activity (37.77% ± 1.30%, 5.0 mg mL −1 ). However, all these LJPs exert weak inhibitory effects against α-amylase. These results show that UET is an effective method for extracting bioactive polysaccharides from seaweed materials. LJP1 and LJP2 can be developed as a potential ingredient in hypoglycemic agents or functional food for the management of diabetes. This study provides scientific evidence and advances in the preparation technology and a hypoglycemic activities evaluation method for seaweed polysaccharides, especially glycosidase inhibition in combination with an antioxidative activity evaluation method.

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Section: Methodsmentioning

confidence: 99%

Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities

Wan

Yang

Cai

et al. 2015

J. Ocean Univ. China

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“…There were few studies focus on these exopolysaccharides composition. Dai et al (2010) reported that glucan, sulfated proteoglycan and sulfated heteropolysaccharide mainly containing glucose were the main components of these exopolysaccharides. Zheng et al (1997) and Dai et al (2010) studies showed that monosaccharide composition of the exopolysaccharides from D.…”

Section: Pressurized Liquid Extraction Is An Emerging Extraction Techmentioning

confidence: 99%

Antiviral compounds obtained from microalgae commonly used as carotenoid sources

et al. 2011

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Pressurized liquid extraction (PLE), an environmentally friendly technique, has been used to obtain antiviral compounds from microalgae commonly used as carotenoids sources: Haematococcus pluvialis and Dunaliella salina. The antiviral properties of PLE extracts (hexane, ethanol and water) were evaluated against herpes simplex virus type 1 (HSV-1) at different stages during viral infection. Pre-treatment of Vero cells with 75 g mL -1 of H. pluvialis ethanol extract inhibited virus infection by approx. 85%, whereas the same concentration of water and hexane extracts reduced the virus infectivity 75% and 50% respectively. D. salina extracts were less effective than H. pluvialis extracts and presented a different behaviour, since water and ethanol extracts produced a similar virus inhibition (65%). Moreover, H. pluvialis ethanol extract was also the most effective against HSV-1 intracellular replication.The antiviral activity of water PLE extracts was found to correlate with polysaccharides, since the polysaccharide-rich fraction isolated from these extracts showed higher antiviral activity than the original water extracts. A GC-MS characterization of the H.pluvialis ethanol extract showed the antiviral activity of this extract could be partially related with the presence of short chain fatty acids, although other compounds could be involved in this activity; meanwhile, in the case of D. salina ethanol extract other compounds seemed to be implied, such as: β-ionone, neophytadiene, phytol, palmitic acid and α-linolenic acid.

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“…Furthermore, HPLC-MS has a great advantage over HPLC-UV for its selectivity which is critical for the detection of these PMP-disaccharides in minor amount. PMP derivatization applied in this experiment could not only benefit the chromatograph separation and ionization in LC-MS, but also enhance the method selectivity by reaction specificity for saccharides (Dai et al, 2010). Therefore, it is unnecessary to further purify polysaccharides from biological samples, and a simple isolation procedure is enough for analysis using this method.…”

Section: Identification Of Uacps In Shellfishesmentioning

confidence: 99%

Characterization of acidic polysaccharides from the mollusks through acid hydrolysis

Cao

Wen

et al. 2015

Carbohydrate Polymers

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Sugar compositional determination of polysaccharides from Dunaliella salina by modified RP-HPLC method of precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone

Cited by 301 publications

References 22 publications

Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities

Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities

Antiviral compounds obtained from microalgae commonly used as carotenoid sources

Characterization of acidic polysaccharides from the mollusks through acid hydrolysis

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