Sugar Catabolism in Aspergillus and Other Fungi Related to the Utilization of Plant Biomass

Khosravi, Claire; Benocci, Tiziano; Battaglia, Evy; Benoit, Isabelle; Vries, Ronald P. de

doi:10.1016/bs.aambs.2014.09.005

Cited by 52 publications

(54 citation statements)

References 103 publications

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“…After release from pectin by A. niger , d ‐galacturonic acid and l ‐rhamnose are catabolized via the d ‐galacturonic acid pathway, involving four genes ( gaaA , gaaB , gaaC and larA ), and the l ‐rhamnose pathway, involving three genes ( lraA , lraB and lraC ) respectively (Khosravi et al ., ). The highest total expression of the d ‐galacturonic acid pathway genes was in the SBP part of the colony (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…org) (Lombard et al, 2014), and their production is mediated by a set of transcriptional activators, responding to specific components of plant biomass (Kowalczyk et al, 2014). These regulators often also control the metabolic pathways converting the released monomeric sugars (Khosravi et al, 2014). It can therefore be hypothesized that the different polysaccharide composition in natural biotopes will result in diverse physiology of A. niger, even within the colony.…”

Section: Introductionmentioning

confidence: 99%

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Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation

Daly

Peng

Mitchell

et al. 2020

Environmental Microbiology

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Summary Saprobic fungi, such as Aspergillus niger, grow as colonies consisting of a network of branching and fusing hyphae that are often considered to be relatively uniform entities in which nutrients can freely move through the hyphae. In nature, different parts of a colony are often exposed to different nutrients. We have investigated, using a multi‐omics approach, adaptation of A. niger colonies to spatially separated and compositionally different plant biomass substrates. This demonstrated a high level of intra‐colony differentiation, which closely matched the locally available substrate. The part of the colony exposed to pectin‐rich sugar beet pulp and to xylan‐rich wheat bran showed high pectinolytic and high xylanolytic transcript and protein levels respectively. This study therefore exemplifies the high ability of fungal colonies to differentiate and adapt to local conditions, ensuring efficient use of the available nutrients, rather than maintaining a uniform physiology throughout the colony.

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation

Daly

Peng

Mitchell

et al. 2020

Environmental Microbiology

View full text Add to dashboard Cite

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“…Hemicelluloses are more diverse in nature, and they are classified in three main types, depending on the backbone: xylan (β-1,4-linked d -xylose), xyloglucan (β-1,4-linked d -glucose), and mannan (β-1,4-linked d -mannose). These backbones are interrupted, branched, or decorated with several different monomers or chains, resulting in different variants of these polymers [28, 29]. Pectin is the third polysaccharide in plant cell wall, but its amount depends on the plant species and tissue.…”

Section: Plant Biomass Composition and Its Degradationmentioning

confidence: 99%

“…Interestingly, regulation of the d -xylose reductase-encoding gene by XYR1 in T. reesei implicates control of XYR1 on d -galactose, lactose, l -arabinose, and d -xylose catabolism, due to the broad specificity and metabolic role of this enzyme [28, 63, 69, 100, 103]. …”

Section: Transcription Factors Directly Involved In Plant Biomass Degmentioning

confidence: 99%

Regulators of plant biomass degradation in ascomycetous fungi

Benocci

Pontes

Zhou

et al. 2017

Biotechnol Biofuels

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Fungi play a major role in the global carbon cycle because of their ability to utilize plant biomass (polysaccharides, proteins, and lignin) as carbon source. Due to the complexity and heterogenic composition of plant biomass, fungi need to produce a broad range of degrading enzymes, matching the composition of (part of) the prevalent substrate. This process is dependent on a network of regulators that not only control the extracellular enzymes that degrade the biomass, but also the metabolic pathways needed to metabolize the resulting monomers. This review will summarize the current knowledge on regulation of plant biomass utilization in fungi and compare the differences between fungal species, focusing in particular on the presence or absence of the regulators involved in this process.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0841-x) contains supplementary material, which is available to authorized users.

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“…These PBD CAZy include as well as the polysaccharide degrading CAZymes, the LMEs and other auxiliary activity (AA) enzymes related to lignin degradation. The relevant carbon catabolism genes in D. squalens were identified by bidirectional BLAST using the carbon catabolism genes from ascomycetes listed in Patyshakuliyeva and colleagues () or from Khosravi and colleagues () (Supporting Information Table S4). CytP450s were those with the InterPro accession IPR001128 (InterPro accessions were also used for the sub‐categories for groups and classes) from the InterProScan (Jones et al ., ) annotations of the Dicsqu464_1 proteins from the JGI.…”

Section: Methodsmentioning

confidence: 99%

Dichomitus squalens partially tailors its molecular responses to the composition of solid wood

Daly

López

Peng

et al. 2018

Environmental Microbiology

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Summary White‐rot fungi, such as Dichomitus squalens, degrade all wood components and inhabit mixed‐wood forests containing both soft‐ and hardwood species. In this study, we evaluated how D. squalens responded to the compositional differences in softwood [guaiacyl (G) lignin and higher mannan content] and hardwood [syringyl/guaiacyl (S/G) lignin and higher xylan content] using semi‐natural solid cultures. Spruce (softwood) and birch (hardwood) sticks were degraded by D. squalens as measured by oxidation of the lignins using 2D‐NMR. The fungal response as measured by transcriptomics, proteomics and enzyme activities showed a partial tailoring to wood composition. Mannanolytic transcripts and proteins were more abundant in spruce cultures, while a proportionally higher xylanolytic activity was detected in birch cultures. Both wood types induced manganese peroxidases to a much higher level than laccases, but higher transcript and protein levels of the manganese peroxidases were observed on the G‐lignin rich spruce. Overall, the molecular responses demonstrated a stronger adaptation to the spruce rather than birch composition, possibly because D. squalens is mainly found degrading softwoods in nature, which supports the ability of the solid wood cultures to reflect the natural environment.

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Sugar Catabolism in Aspergillus and Other Fungi Related to the Utilization of Plant Biomass

Cited by 52 publications

References 103 publications

Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation

Colonies of the fungus Aspergillus niger are highly differentiated to adapt to local carbon source variation

Regulators of plant biomass degradation in ascomycetous fungi

Dichomitus squalens partially tailors its molecular responses to the composition of solid wood

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