Sugar Beet (Beta vulgaris L.)

Kagami, Hiroyo; Kurata, Michio; Matsuhira, Hiroaki; Taguchi, Kazunori; Mikami, Tetsuo; Tamagake, Hideto; Kubo, Tomohiko

doi:10.1007/978-1-4939-1695-5_27

Cited by 19 publications

(9 citation statements)

References 12 publications

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“…The expression of the transgene was driven by the cauliflower mosaic virus (CaMV) 35S promoter. Sugar beet line NK‐219 mm‐CMS, a CMS line, was used in these analyses because it is the only sugar beet line available to us that can be used for transgenic analyses (Kagami et al ., ).…”

Section: Resultsmentioning

confidence: 97%

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Post‐translational mechanisms are associated with fertility restoration of cytoplasmic male sterility in sugar beet (Beta vulgaris)

et al. 2015

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SUMMARYGenetic conflict between cytoplasmically inherited elements and nuclear genes arising from their different transmission patterns can be seen in cytoplasmic male sterility (CMS), the mitochondrion-encoded inability to shed functional pollen. CMS is associated with a mitochondrial open reading frame (ORF) that is absent from non-sterility inducing mitochondria (S-orf). Nuclear genes that suppress CMS are called restorer-of-fertility (Rf) genes. Post-transcriptional and translational repression of S-orf mediates the molecular action of Rf that encodes a class of RNA-binding proteins with pentatricopeptide repeat (PPR) motifs. Besides the PPR-type of Rfs, there are also non-PPR Rfs, but the molecular interactions between non-PPR Rf and S-orf have not been described. In this study, we investigated the interaction of bvORF20, a non-PPR Rf from sugar beet (Beta vulgaris), with preSatp6, the S-orf from sugar beet. Anthers expressing bvORF20 contained a protein that interacted with preSATP6 protein. Analysis of anthers and transgenic calli expressing a FLAGtagged bvORF20 suggested the binding of preSATP6 to bvORF20. To see the effect of bvORF20 on pre-SATP6, which exists as a 250-kDa protein complex in CMS plants, signal bands of preSATP6 in bvORF20-expressing and non-expressing anthers were compared by immunoblotting combined with Blue Native polyacrylamide gel electrophoresis. The signal intensity of the 250-kDa band decreased significantly, and 200-and 150-kDa bands appeared in bvORF20-expressing anthers. Transgenic callus expressing bvORF20 also generated the 200-and 150-kDa bands. The 200-kDa complex is likely to include both preSATP6 and bvORF20. Post-translational interaction between preSATP6 and bvORF20 appears to alter the higher order structure of preSATP6 that may lead to fertility restoration in sugar beet.

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Section: Resultsmentioning

confidence: 97%

“…TK‐81 mm‐O has the same nuclear genotype as TK‐81 mm‐CMS but is male fertile because of non‐sterilizing mitochondria (Satoh et al ., ). NK‐219 mm‐CMS is another MS line with CMS mitochondria, but its genotype is known to be suitable for generating transgenic sugar beet plants (Kagami et al ., ).…”

Section: Methodsmentioning

confidence: 97%

Post‐translational mechanisms are associated with fertility restoration of cytoplasmic male sterility in sugar beet (Beta vulgaris)

et al. 2015

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“…These attributes enabled us to develop an Agrobacterium -mediated transformation system for sugar beet [3, 4]. Using this system, molecular analyses of several genes have been completed [5–7].…”

Section: Resultsmentioning

confidence: 99%

“…We investigated the frequency of callus formation in these F 1 populations and five parental lines. As detailed in [3, 4], surface-sterilized seeds were sown on solid medium in vitro, and plantlets were grown. We removed two leaves from each plantlet, cut each of them into six pieces, and placed the explants on callus-inducing medium.…”

Section: Resultsmentioning

confidence: 99%

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Efficient callus formation and plant regeneration are heritable characters in sugar beet (Beta vulgaris L.)

et al. 2016

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BackgroundObtaining dedifferentiated cells (callus) that can regenerate into whole plants is not always feasible for many plant species. Sugar beet is known to be recalcitrant for dedifferentiation and plant regeneration. These difficulties were major obstacles for obtaining transgenic sugar beets through an Agrobacterium-mediated transformation procedure. The sugar beet line ‘NK-219mm-O’ is an exceptional line that forms callus efficiently and is easy to regenerate, but the inheritance of these characters was unknown. Another concern was whether these characters could coexist with an annual habitat that makes it possible to breed short life-cycle sugar beet suitable for molecular genetic analysis.FindingsFive sugar beet lines including NK-219mm-O were crossed with each other and subjected to in vitro culture to form callus. F1s with a NK-219mm-O background generally formed callus efficiently compared to the others, indicating that efficient callus formation is heritable. The regeneration potential was examined based on the phenotypes of calli after placement on regeneration medium. Five phenotypes were observed, of which two phenotypes regenerated shoots or somatic embryo-like structures. Vascular differentiation was evident in regenerable calli, whereas non-regenerable calli lacked normally developed vascular tissues. In a half-diallel cross, the callus-formation efficiency and the regeneration potential of reciprocal F1s progeny having a NK-219mm-O background were high. Finally, we crossed NK-219mm-O with an annual line that had a poor in vitro performance. The callus-formation efficiency and the regeneration potential of reciprocal F1 were high. The regenerated plants showed an annual habitat.ConclusionsEfficient callus formation and the high plant regeneration potential of NK-219mm-O were inherited and expressed in the F1. The annual habitat does not impair these high in vitro performances.Electronic supplementary materialThe online version of this article (doi:10.1186/s41065-016-0015-z) contains supplementary material, which is available to authorized users.

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Naturally Occurring Coloring and Flavoring Agents

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2020

Plant-Derived Bioactives

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Sugar Beet (Beta vulgaris L.)

Cited by 19 publications

References 12 publications

Post‐translational mechanisms are associated with fertility restoration of cytoplasmic male sterility in sugar beet (Beta vulgaris)

Post‐translational mechanisms are associated with fertility restoration of cytoplasmic male sterility in sugar beet (Beta vulgaris)

Efficient callus formation and plant regeneration are heritable characters in sugar beet (Beta vulgaris L.)

Naturally Occurring Coloring and Flavoring Agents

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