Sugar and Light Effects on the Condition of the Photosynthetic Apparatus of Arabidopsis thaliana Cultured in vitro

Eckstein, Aleksandra; Zięba, P.; Gabryś, Halina

doi:10.1007/s00344-011-9222-z

Cited by 80 publications

(58 citation statements)

References 33 publications

(19 reference statements)

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“…The lower pigment accumulation observed in tissues from the culture media supplemented with 30 g L -1 sucrose and NAA, regardless the closure type, is a typical response found in the literature, being attributed to the effects of sucrose on biosynthesis of photosynthetic pigments, which result in inhibition of chlorophyll biosynthesis and in vitro photosynthesis (Eckstein et al, 2012). Plantlets grown without sucrose show a higher photosynthetic rate because this is the only route to carbon fixation (Iarema et al, 2012).…”

Section: Discussionmentioning

confidence: 61%

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

et al. 2016

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This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA 3 or 0.2 µM NAA, sucrose at 15 or 30 g L -1 , in test tubes which allowed gas exchange or in a hermetically sealed system, and 180 days after acclimatization. The in vitro maintenance in hermetically sealed flasks, with GA 3 and 15 g L -1 sucrose had adverse metabolic effects, which was demonstrated by the lower proline and photosynthetic pigments accumulation and by the increase in antioxidant enzymes activities. After acclimatization, differences for proline and photosynthetic pigments were no longer found and the enzymatic activities ranged unevenly. The results suggest that the in vitro cultivation in media with 0.2 µM NAA and 30 g L -1 sucrose, in test tubes capped with closures which allowed gas exchange, is more suitable for micropropagation of P. encholirioides, providing a prolonged maintenance of in vitro cultures and plantlets with superior quality for ex vitro development.Keywords: enzymatic activity, oxidative stress, micropropagation, proline. Niveis de prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos durante o crescimento in vitro e aclimatização de Pitcairnia encholirioides L.B. Sm. (Bromeliaceae) ResumoEste trabalho objetivou avaliar a contribuição da prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos na propagação in vitro e aclimatização de Pitcairnia encholirioides, uma bromélia criticamente ameaçada de extinção. As análises foram realizadas após 150 dias de cultivo in vitro em meio MS suplementado com 10 µM de GA 3 ou 0,2 µM de ANA, 15 ou 30 g L -1 de sacarose, em tubos de ensaio que permitiam trocas gasosas ou em sistema hermeticamente vedado, e também 180 dias após aclimatização. A manutenção in vitro em frascos hermeticamente fechados, com GA 3 e 15 g L -1 de sacarose apresentou efeito metabólico adverso, demonstrado pelo menor acúmulo de prolina e pigmentos fotossintéticos e também pelo aumento das atividades de enzimas antioxidantes. Após aclimatização, as diferenças para prolina e pigmentos fotossintéticos não foram mais encontradas e as atividades enzimáticas variaram de maneira desuniforme. Os resultados sugerem que o cultivo in vitro em meio com 0,2 µM de ANA e 30 g L -1 de sacarose, em tubos fechados com tampas que permitem trocas gasosas, é mais adequado para a micropropagação de P. encholirioides, proporcionando uma manutenção prolongada das culturas in vitro e plântulas com qualidade superior para o desenvolvimento ex vitro.Palavras-chave: atividade enzimática, estresse oxidativo, micropropagação, prolina.

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Section: Discussionmentioning

confidence: 61%

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

et al. 2016

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“…Therefore we can infer that the absence of nutrients combined with genetic factors may cause higher rates of abnormal plants. When cultivating Arabidopsis thaliana using different concentrations of sugar, ECKSTEIN et al (2012) observed that plants cultivated without this carbohydrate were slower to develop and did not reach the generative phase. Some species do not depend on nutritive medium, for example the genipap, which showed a higher rate of germination and formation of normal seedlings when cultivated without salts and sucrose (ALMEIDA et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Germinação in vitro de variedades de rosa-do-deserto

Varella

Silva

Cruz

et al. 2015

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The drought stress resistance is a characteristic of the desert rose and its estimable beauty flowers, which gave it great relevance in the ornamental market. However, the desert rose production and germination is hampered by possible sterility of their male and female flowers and frequent problems in pollination, so the tissue culture is a promising alternative to the propagation of these plants. This study aimed to evaluate the effect of gibberellic acid on four commercial varieties of desert rose (Adenium obesum) cultivated in vitro. The seeds of the varieties 'Orange Pallet', 'Carnation violet', 'Diamond ring' and 'Vermiliont' were sterilized and inoculated on Water + Agar (T0), medium MS (T1), ½ MS (T2), MS + 0.25 mg L -1 GA 3 (T3), MS + 0.5 mg L -1 GA 3 (T4), ½ MS + 0.25 mg L -1 GA 3 (T5), ½ MS 0.5 mg L -1 GA 3 (T6). The seeds germination of A. obesum was initiated on the fourth day of cultivation and on the tenth day was possible to observe the expansion of the cotyledons and leaf expansion with subsequent development of early secondary root. The 'Orange pallet' variety germinated 100% of seeds on water + agar and MS ½ + 0.5 mg L -1 of GA 3 . For 'Diamond Ring' and 'Carnation violet' the highest rate of germination occurred in treatments MS ½; 0.25 mg L -1 GA 3; MS + 0.5 mg L -1 GA 3 MS ½ + 0.5 mg L -1 GA 3 averaging 80% and 70%, respectively. For 'Vermiliont' the best response was in MS and MS ½ + 0.5 mg L -1 GA 3 ranging between 70-90% germinated embryos. It was registered different malformations in all treatments like absence of roots and apexes during seedling development. The concentrations of GA 3 did not affect significantly the seed germination.

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“…The effects of sugars on plant aquaporins (and water conductance) have recently been examined; it was observed that glucose reduces the movement of water from the xylem to the mesophyll [41], which could also affect leaf growth. It has also been shown that sugars (such as, glucose and sucrose) can affect the movement of cellular organelles, including chloroplasts [42,43]. Trehalose (at a concentration about 25 mM) inhibited root elongation in A. thaliana seedlings, whereas sucrose did not affect this process [44].…”

Section: Introductionmentioning

confidence: 99%

Regulatory roles of sugars in plant growth and development

Ciereszko

2018

Acta Soc Bot Pol

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In recent years, several studies have focused on the factors and mechanisms that regulate plant growth and development, as well as the functioning of signaling pathways in plant cells, unraveling the involvement of sugars in the processes regulating such growth and development. Saccharides play an important role in the life of plants: they are structural and storage substances, respiratory substrates, and intermediate metabolites of many biochemical processes. Sucrose is the major transport form of assimilates in plants. Sugars can also play an important role in the defense reactions of plants. However, it has been shown that glucose, sucrose, or trehalose-6-phosphate (Tre6P) can regulate a number of growth and metabolic processes, acting independently of the basal functions; they can also act as signaling molecules. Changes in the concentration, qualitative composition, and transport of sugars occur continuously in plant tissues, during the day and night, as well as during subsequent developmental stages. Plants have developed an efficient system of perception and transmission of signals induced by lower or higher sugar availability. Changes in their concentration affect cell division, germination, vegetative growth, flowering, and aging processes, often independently of the metabolic functions. Currently, the mechanisms of growth regulation in plants, dependent on the access to sugars, are being increasingly recognized. The plant growth stimulating system includes hexokinase (as a glucose sensor), trehalose-6-phosphate, and TOR protein kinase; the lack of Tre6P or TOR kinase inhibits the growth of plants and their transition to the generative phase. It is believed that the plant growth inhibition system consists of SnRK1 protein kinases and C/S1 bZIP transcription factors. The signal transduction routes induced by sugars interact with other pathways in plant tissues (for example, hormonal pathways) creating a complex communication and signaling network in plants that precisely controls plant growth and development.

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Sugar and Light Effects on the Condition of the Photosynthetic Apparatus of Arabidopsis thaliana Cultured in vitro

Cited by 80 publications

References 33 publications

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

Germinação in vitro de variedades de rosa-do-deserto

Regulatory roles of sugars in plant growth and development

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