Sudies of O-specific polysaccharide chains of Pseudomonassolanacearum lipopolysaccharides consisting of structually different repeating units.

Kocharova, Nina A.; Knirel, Yuriy A.; Shashkov, Alexander S.; Nifant'ev, Nikolay E.; Kochetkov, Nikolay K.; Varbanets, L.D.; Moskalenko, N. V.; Brovarskaya, O. S.; Muras, V. A.; Young, J. M.

doi:10.1016/0008-6215(93)84006-r

Cited by 23 publications

(13 citation statements)

References 15 publications

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“…Mild acid hydrolysis of the LPS of R. solanacearum Toudk-2 released the O-polysaccharide and higher oligosaccharides. The former belongs to a large group of R. solanacearum Opolysaccharides 6 synthesized by polymerization of the same preassembled lipid-linked R-Rha-(1f2)-R-Rha-(1f3)-R-Rha-(1f3)-GlcNAc tetrasaccharide O-unit called biological O-unit. 15 The resultant O-polysaccharides may differ from each other in the linkage between the O-units, which is R1f2, R1f3, or β1f3, 6 depending on the specificity of O-antigen polymerase that catalyzes the polymerization.…”

Section: Discussionmentioning

confidence: 99%

Structure of the Oligosaccharide Chain of the SR-Type Lipopolysaccharide of Ralstonia solanacearum Toudk-2

et al. 2008

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Aiming at improving classification and taxonomy of Gram-negative phytopathogenic bacteria, we studied the structure of the lipopolysaccharide of Ralstonia solanacearum. Mild acid hydrolysis of the lipopolysaccharide of strain Toudk-2 followed by gel chromatography resulted in an O-polysaccharide and two oligosaccharide fractions. The smallest-size oligosaccharide fraction was studied by sugar analysis, high-resolution electrospray ionization mass spectrometry, and, after fractionation by anion-exchange chromatography on HiTrap Q, by one- and two-dimensional (1)H and (13)C NMR spectroscopy. It was found that the isolated oligosaccharides consist of the lipopolysaccharide core with one O-polysaccharide repeat (O-unit) attached. The core exists in two major glycoforms differing from each other in a lateral octulosonic acid residue, which is either D-glycero-D-talo-oct-2-ulosonic acid or 3-deoxy-D-manno-oct-2-ulosonic acid. A peculiar feature of the core is the occurrence of 4-amino-4-deoxy-L-arabinose nonstoichiometrically linked to a heptose residue. The full structures of the core and the biological O-unit as well as the site of the attachment of the O-unit to the core were established.

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Section: Discussionmentioning

confidence: 99%

Structure of the Oligosaccharide Chain of the SR-Type Lipopolysaccharide of Ralstonia solanacearum Toudk-2

et al. 2008

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“…B. pseudomallei [18,191, and a number of other organims, e.g. Acinetobacter baumannii 01 1, Serratia plymuthica, Klebsiella pneumoniae 0 1 , 0 2 and 0 8 , Pseudomonas aeruginosa, Salmonella boecker [ 10,201, Salmonella madelia, which was shown to produce three structurally different 0-polysaccharides [21], a feature also described for Campylobacter jejuni 0 : 2 3 [22] and Ralstonia (Burkholderia or Pseudomonas) solanacearum strain 8072 [23]. Similarly, Salmonella zuerich D3 has been shown to produce four types of 0-antigenic polymers sharing the same basic sequence [24].…”

Section: Discussionmentioning

confidence: 99%

Structural Elucidation of two Polysaccharides Present in the Lipopolysaccharide of a Clinical Isolate of Burkholderia Cepacia

Cérantola

Montrozier

1997

European Journal of Biochemistry

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Based on the sugar composition, methylation analyses and Smith degradation, supported by NMR spectroscopic analyses and fast-atom-bombardment MS experiments, the lipopolysacharide produced by a clinical isolate of Burkholderia cepacia was shown to contain two distinct polymers, both with linear trisaccharide repeating units ; a major, containing D-rhamnose and D-galactose residues (2 : 1) with the structureand a minor repeating unit, constituted by D-rhamnosyl residues, with the structureKeywards: Burkholderia cepacia; lipopolysaccharide; D-rhamnose; cystic fibrosis; fast-atom-bombardinent MS.Burkholderiu cepacia, previously known as Pseudomonas cepacia, is the type species of the new genus Burkholderia described by Yabuuchi et al. [l]. This bacterium, originally considered as a saprophyte causing soft rot of onions [2], has emerged during the last decade as an important opportunistic pathogen in nosocomial infections and notably as a real threat in patients with cystic fibrosis (CF) due to its association, in some cases, with the cepacia syndrome, i.e. a rapid and fatal decline in pulmonary functions [3-51.In contrast to Pseudomonus ueruginosa, another well-known CF pathogen, little information is available on the molecular basis of the pathogenicity of B. cepucia. Several cellular and extracellular products have been listed as potential virulence factors : these include iron-chelating siderophores, outer membrane proteins, hemolysin, lipases, proteases, exopolysaccharides (EPS) and lipopolysaccharides (LPS) [6-81. Their precise role, however, as pathogenic determinants remains unclear and needs further investigation.In order to distinguish strains of B. cepucia, due to the marked phenotypic variability observed for clinical isolates of this organism, several typing methods have been proposed, notably those based on 0-antigenic polysaccharides of the LPS [9]. The aim of these studies is to understand the epidemiology of B. cepacia and to permit a better biological control of this bacterium. The chemical structures of the 0-polysaccharide repeating units of LPS have been elucidated for many strains of B. cepacia. They were shown to be rather simple, compared with other organisms, with linear disaccharide or trisaccharide repeating Here, we describe the structure of two D-rhamnose-containing LPS-related polymers produced by a clinical isolate of B. cepacia. MATERIALS AND METHODSGrowth conditions, extraction, purification and fractionation of the bacterial LPS. A B. cepacia clinical isolate was obtained from Dr T. L. Pitt (Central Public Health Laboratory, Colindale, London). An 18-h bacterial culture was inoculated on a Pseudomonas isolation agar medium (PTA, Difco) containing 2 % glycerol and incubated for 72 h at 37°C. LPS was extracted from the dry cells by the hot aqueous phenol method [Ill. The aqueous phase was then extensively dialysed against distilled water (4 days at 4°C) to remove phenol and freeze dried. The material was then dissolved in distilled water, digested with nucleases (DNAse I and RNAse A)...

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“…Remarkably, structure 1 has also been reported for the OPS of human pathogen Serratia marcescens O22 [17] and differs from the OPS of Shigella flexneri serotype Y only by the anomeric configuration of the GlcNAc residue [18]. Structure 2 has been identified in LPS of strains B. solanacearum ICMP 767, 7864, 7942, and 7955 [15] [16] [19].…”

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confidence: 96%

Host and Non‐Host Plant Response to Bacterial Wilt in Potato: Role of the Lipopolysaccharide Isolated from Ralstonia solanacearum and Molecular Analysis of Plant–Pathogen Interaction

Esposito

Ovchinnikova

Barone

et al. 2008

Chemistry & Biodiversity

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Ralstonia solanacearum is one of the most devastating phytopathogenic bacteria, in particular its race 3. This microorganism is the causal agent of destructive diseases of different crops including tomato and potato. An important aspect of the interaction between this pathogen, and the host and non-host plants was its biochemical and molecular basis. Thus, the lipopolysaccharides (LPS) were extracted from the R. solanacearum cell wall, purified, and the O-specific polysaccharide (OPS) was isolated and chemically characterized by compositional analyses and NMR spectroscopy. The OPS was constituted of two linear polymers of an approximate ratio of 3 : 1, both of which were built up from three rhamnose and one N-acetylglucosamine residues and differed only in the substitution of one rhamnose residue. The LPS inhibited the hypersensitivity reaction (HR) in non-host tobacco plants and induced localized resistance in host potato plants, both of which were pre-treated with the LPS before being inoculated with the pathogen. A cDNA-AFLP approach was used to study transcriptome variation during the resistant and susceptible interactions. This revealed the presence of metabolites specifically expressed in the S. commersonii-resistant genotypes, which could be involved in the plant-pathogen incompatible reaction. Furthermore, a specific EST collection of the Ralstonia-potato interaction has been built up.

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Sudies of O-specific polysaccharide chains of Pseudomonassolanacearum lipopolysaccharides consisting of structually different repeating units.

Cited by 23 publications

References 15 publications

Structure of the Oligosaccharide Chain of the SR-Type Lipopolysaccharide of Ralstonia solanacearum Toudk-2

Structure of the Oligosaccharide Chain of the SR-Type Lipopolysaccharide of Ralstonia solanacearum Toudk-2

Structural Elucidation of two Polysaccharides Present in the Lipopolysaccharide of a Clinical Isolate of Burkholderia Cepacia

Host and Non‐Host Plant Response to Bacterial Wilt in Potato: Role of the Lipopolysaccharide Isolated from Ralstonia solanacearum and Molecular Analysis of Plant–Pathogen Interaction

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