Sucrose synthase is involved in the carbohydrate metabolism-based regulation of seed dormancy release in<i>Pyrus calleryana</i>Decne

Liu, Xiao; Huang, Xv; Kong, Xiao-Xiong; Zhang, Jing; Wang, Jizhong; Yang, Meiling; Chun-lei, Wang

doi:10.1080/14620316.2020.1740612

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…Additionally, enzymes and corresponding genes associated with starch-sucrose conversion have been widely studied in many species [20,21]. Emerging findings also report that sucrose and starch are hydrolyzed into glucose by sucrose synthase (SUS/SS) and α-amylase (AMY) during pear seed dormancy release and the inhibition of PcaSS8 blocks pear seeds from germinating [22].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and Metabolite Conjoint Analysis Reveals the Seed Dormancy Release Process in Callery Pear

Zhang

Qian

Bian

et al. 2022

IJMS

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Seed dormancy transition is a vital developmental process for seedling propagation and agricultural production. The process is precisely regulated by diverse endogenous genetic factors and environmental cues. Callery pear (Pyrus calleryana Decne) is an important rootstock species that requires cold stratification to break seed dormancy, but the mechanisms underlying pear seed dormancy release are not yet fully understood. Here, we analyzed the transcriptome profiles at three different stages of cold stratification in callery pear seeds using RNA sequencing combined with phytohormone and sugar content measurements. Significant alterations in hormone contents and carbohydrate metabolism were observed and reflected the dormancy status of the seeds. The expressions of genes related to plant hormone metabolism and signaling transduction, including indole-3-acetic acid (IAA) biosynthesis (ASAs, TSA, NITs, YUC, and AAO) genes as well as several abscisic acid (ABA) and gibberellic acid (GA) catabolism and signaling transduction genes (CYP707As, GA2ox, and DELLAs), were consistent with endogenous hormone changes. We further found that several genes involved in cytokinin (CTK), ethylene (ETH), brassionolide (BR), and jasmonic acid (JA) metabolism and signaling transduction were differentially expressed and integrated in pear seed dormancy release. In accordance with changes in starch and soluble sugar contents, the genes associated with starch and sucrose metabolism were significantly up-regulated during seed dormancy release progression. Furthermore, the expression levels of genes involved in lipid metabolism pathways were also up-regulated. Finally, 447 transcription factor (TF) genes (including ERF, bHLH, bZIP, NAC, WRKY, and MYB genes) were observed to be differentially expressed during seed cold stratification and might relate to pear seed dormancy release. Our results suggest that the mechanism underlying pear seed dormancy release is a complex, transcriptionally regulated process involving hormones, sugars, lipids, and TFs.

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Section: Introductionmentioning

confidence: 99%

Transcriptome and Metabolite Conjoint Analysis Reveals the Seed Dormancy Release Process in Callery Pear

Zhang

Qian

Bian

et al. 2022

IJMS

Self Cite

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“…One explanation is that sugar production and consumption varied among the types. In the 50 days for dormancy release of Pyrus calleryana seeds, sucrose content was always stable, while it decreased at 60 d, and the content of glucose and fructose were not changed in the first 20 d but increased at 50 d [18,19]. The increase in amylase activities is important for seed germination, and exogenous intervention of their activities and gene expression can inhibit germination [20][21][22].…”

Section: Discussionmentioning

confidence: 99%

Morphology and Physiology Response to Stratification during Seeds Epicotyl Dormancy Breaking of Paeonia emodi Wall. ex Royle

Wan

Zhang

Liu³

et al. 2022

Agronomy

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Paeonia emodi is a type of wild herbaceous peony with high ornamental and breeding value. Cold stratification is the only method to break its seed epicotyl dormancy to date, however, the key physiological factors during this process are not clear. In this study, rooted seeds of P. emodi were treated with 4 °C stratification, and the changes of the embryo, four nutrients, and two endogenous hormones in the seeds were investigated. The results showed that the plumule elongated at S6 (i.e., ten weeks of cold stratification), and grew to nearly the same length as the cotyledon at S9. Cold stratification increased starch consumption, significantly decreased soluble sugar content in the later stages, and decreased soluble protein content at S9, but it did not influence crude fat content. The activities of α-amylase and β-amylase increased significantly at S4 and S4 to S6, respectively. At S8 and S9, acid protease activity increased, and the increase in lipase activity continued throughout the whole process. At the same time, the ABA content decreased significantly after S6; from this stage, the ABA/GA3 ratio gradually decreased compared with that of the control, and the difference was significant at S9. Correlation analysis showed that the ABA/GA3 ratio was significantly correlated with starch content and α-amylase activity. It can be concluded that both carbohydrates and proteins were the energy supply for the epicotyl dormancy breaking of P. emodi seeds, rather than crude fat. Cold stratification promoted substance transformation by increasing the corresponding enzyme activities. The balance of ABA and GA3 suggested the key stage for the release of dormancy.

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“…Our results showed that two SSs increased in abundance by HEHP and the activity of SS was also increased by HEHP treatment during imbibition. A recent study also indicated that SS participates in the release of seed dormancy ( Liu et al, 2020 ). Early activation of respiratory pathways was also found, which is responsible for providing energy for cell activities and the carbon skeleton of macromolecular biosynthesis during seed germination.…”

Section: Discussionmentioning

confidence: 99%

Carrot (Daucus carota L.) Seed Germination Was Promoted by Hydro-Electro Hybrid Priming Through Regulating the Accumulation of Proteins Involved in Carbohydrate and Protein Metabolism

Zhao

Zou

Jia³

et al. 2022

Front. Plant Sci.

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Asynchronized and non-uniform seed germination is causing obstacles to the large-scale cultivation of carrot (Daucus carota L.). In the present study, the combination of high voltage electrostatic field treatment (EF) with hydropriming (HYD), namely hydro-electro hybrid priming (HEHP), significantly improved all germination indicators of carrot seeds, and the promoting effect was superior to that of the HYD treatment. A tandem mass tags (TMT)-based proteomic analysis identified 4,936 proteins from the seeds, and the maximum number of differentially abundant proteins (DAPs) appeared between CK and HEHP. KEGG analysis revealed that the upregulated DAPs were mainly enriched in the pathways related to protein synthesis and degradation such as “ribosome” and “proteasome,” while the downregulated DAPs were mainly enriched in photosynthesis-related pathways. Furthermore, the maximum DAPs were annotated in carbohydrate metabolism. Some proteins identified as key enzymes of the glyoxylate cycle, the tricarboxylate cycle, glycolysis and the pentose phosphate pathway showed enhanced abundance in priming treatments. The activities of several key enzymes involved in carbohydrate metabolism were also enhanced by the priming treatments, especially the HEHP treatment. Real-time quantitative PCR (qRT-PCR) analysis revealed that the effect of priming is mainly reflected before sowing. In conclusion, the optimal effect of HEHP is to regulate the synthesis and degradation of proteins in seeds to meet the requirements of germination and initiate the utilization of seed storage reserves and respiratory metabolism. The present work expanded the understanding of the response mechanism of carrot seed germination to priming and the biological effects of high voltage electrostatic field.

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Sucrose synthase is involved in the carbohydrate metabolism-based regulation of seed dormancy release inPyrus calleryanaDecne

Cited by 6 publications

References 38 publications

Transcriptome and Metabolite Conjoint Analysis Reveals the Seed Dormancy Release Process in Callery Pear

Transcriptome and Metabolite Conjoint Analysis Reveals the Seed Dormancy Release Process in Callery Pear

Morphology and Physiology Response to Stratification during Seeds Epicotyl Dormancy Breaking of Paeonia emodi Wall. ex Royle

Carrot (Daucus carota L.) Seed Germination Was Promoted by Hydro-Electro Hybrid Priming Through Regulating the Accumulation of Proteins Involved in Carbohydrate and Protein Metabolism

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