Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen

Domínguez, Pía Guadalupe; Donev, Evgeniy N.; Derba‐Maceluch, Marta; Bünder, Anne; Hedenström, Mattias; Tomášková, Ivana; Mellerowicz, Ewa J.; Niittylä, Totte

doi:10.1111/nph.16721

Cited by 26 publications

(23 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SUS activity is highly associated with C allocation, biomass accumulation, and sink strength ( Stein & Granot, 2019 ). For instance, the deletion or suppression of the SUS gene decreases maize seed weight ( Chourey et al, 1998 ), reduces pea seed mass ( Craig et al, 1999 ), leads to tomato fruit setting abnormality ( D’Aoust, Yelle & Nguyen-Quoc, 1999 ), inhibits stem thickening in Populus tomentosa ( Li et al, 2020 ), and reduces the stem height, diameter, and biomass in aspen ( Dominguez et al, 2021 ). The overexpression of SUS increases the growth rate and facilitates plant biomass accumulation in Arabidopsis ( Xu & Joshi, 2010 ), promotes cellulose biosynthesis and increases the lodging resistance in tobacco stem ( Wei et al, 2015 ), and accelerates vegetative growth, thickens the secondary cell wall, and increases the stem breaking force in poplar ( Li et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and expression analysis of the sucrose synthase gene family in pomegranate (Punica granatum L.)

Liu

Zheng

2022

PeerJ

View full text Add to dashboard Cite

Background Sucrose synthase (SUS, EC 2.4.1.13) is one of the major enzymes of sucrose metabolism in higher plants. It has been associated with C allocation, biomass accumulation, and sink strength. The SUS gene families have been broadly explored and characterized in a number of plants. The pomegranate (Punica granatum) genome is known, however, it lacks a comprehensive study on its SUS genes family. Methods PgSUS genes were identified from the pomegranate genome using a genome-wide search method. The PgSUS gene family was comprehensively analyzed by physicochemical properties, evolutionary relationship, gene structure, conserved motifs and domains, protein structure, syntenic relationships, and cis-acting elements using bioinformatics methods. The expression pattern of the PgSUS gene in different organs and fruit development stages were assayed with RNA-seq obtained from the NCBI SRA database as well as real-time quantitative polymerase chain reaction (qPCR). Results Five pomegranate SUS genes, located on four different chromosomes, were divided into three subgroupsaccording to the classification of other seven species. The PgSUS family was found to be highly conserved during evolution after studying the gene structure, motifs, and domain analysis. Furthermore, the predicted PgSUS proteins showed similar secondary and tertiary structures. Syntenic analysis demonstrated that four PgSUS genes showed syntenic relationships with four species, with the exception of PgSUS2. Predictive promoter analysis indicated that PgSUS genes may be responsive to light, hormone signaling, and stress stimulation. RNA-seq analysis revealed that PgSUS1/3/4 were highly expressed in sink organs, including the root, flower, and fruit, and particularly in the outer seed coats. qPCR analysis showed also that PgSUS1, PgSUS3, and PgSUS4 were remarkably expressed during fruit seed coat development. Our results provide a systematic overview of the PgSUS gene family in pomegranate, developing the framework for further research and use of functional PgSUS genes.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification and expression analysis of the sucrose synthase gene family in pomegranate (Punica granatum L.)

Liu

Zheng

2022

PeerJ

View full text Add to dashboard Cite

show abstract

“…It should be noted however that Arabidopsis sus1sus4 mutants exhibit growth defects under hypoxia pointing to the importance of the SUS pathway under stress conditions (Bieniawska et al, 2007). A role for SUS in plant stress tolerance was recently also supported by the growth defects observed in hybrid aspen SUSRNAi lines grown under natural conditions for five years (Dominguez et al, 2021). Table S1.…”

Section: Resultsmentioning

confidence: 99%

Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis

Wang

Viljamaa

Hodek

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Biosynthesis of plant cell walls requires UDP-glucose as the substrate for cellulose biosynthesis, and as an intermediate for the synthesis of other matrix polysaccharides. The sucrose cleaving enzyme sucrose synthase (SUS) is thought to have a central role in UDP-glucose biosynthesis, and a long held and much debated hypothesis postulates that SUS is required to supply UDP-glucose to cellulose biosynthesis. To investigate the role of SUS in cellulose biosynthesis of Arabidopsis thaliana we characterized mutants in which four, or all six Arabidopsis SUS genes were disrupted. These sus mutants showed no growth phenotypes, vascular tissue cell wall defects or changes in cellulose content. Moreover, the UDP-glucose content of rosette leaves of the sextuple sus mutants was increased by approximately 20% compared to wild type. It can thus be concluded that cellulose biosynthesis is able to employ alternative UDP-glucose biosynthesis pathway(s), and thereby the model of SUS requirement for cellulose biosynthesis in Arabidopsis can be refuted.

show abstract

“…Tracking the carbon flux in transgenic plants is critical for understanding the function of CDR-related genes. To investigate the impact of sucrose synthase on carbon allocation and carbon flow at the tissue and whole tree levels, the source leaves, phloem, developing wood, and roots of transgenic hybrid aspen (P. tremula × P. tremuloides) lines, with the expression of sucrose synthase gene repressed by RNAi, were analyzed using a combination of metabolite profiling, 13 CO 2 pulse labelling experiments, and long-term field tests [158]. These types of data can be very useful for metabolic modeling in the "learn" phase of a DBTL cycle.…”

Section: Identification Of New Biological Parts For Cdr Engineering In Terrestrial Plantsmentioning

confidence: 99%

Biological Parts for Plant Biodesign to Enhance Land-Based Carbon Dioxide Removal

Yang

Liu

et al. 2021

BioDesign Res

View full text Add to dashboard Cite

A grand challenge facing society is climate change caused mainly by rising CO2 concentration in Earth’s atmosphere. Terrestrial plants are linchpins in global carbon cycling, with a unique capability of capturing CO2 via photosynthesis and translocating captured carbon to stems, roots, and soils for long-term storage. However, many researchers postulate that existing land plants cannot meet the ambitious requirement for CO2 removal to mitigate climate change in the future due to low photosynthetic efficiency, limited carbon allocation for long-term storage, and low suitability for the bioeconomy. To address these limitations, there is an urgent need for genetic improvement of existing plants or construction of novel plant systems through biosystems design (or biodesign). Here, we summarize validated biological parts (e.g., protein-encoding genes and noncoding RNAs) for biological engineering of carbon dioxide removal (CDR) traits in terrestrial plants to accelerate land-based decarbonization in bioenergy plantations and agricultural settings and promote a vibrant bioeconomy. Specifically, we first summarize the framework of plant-based CDR (e.g., CO2 capture, translocation, storage, and conversion to value-added products). Then, we highlight some representative biological parts, with experimental evidence, in this framework. Finally, we discuss challenges and strategies for the identification and curation of biological parts for CDR engineering in plants.

show abstract

Sucrose synthase determines carbon allocation in developing wood and alters carbon flow at the whole tree level in aspen

Cited by 26 publications

References 69 publications

Identification and expression analysis of the sucrose synthase gene family in pomegranate (Punica granatum L.)

Identification and expression analysis of the sucrose synthase gene family in pomegranate (Punica granatum L.)

Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis

Biological Parts for Plant Biodesign to Enhance Land-Based Carbon Dioxide Removal

Contact Info

Product

Resources

About