Sucrose Represses the Developmentally Controlled Transient Activation of the Plastocyanin Gene in Arabidopsis thaliana Seedlings

Dijkwel, Paul P.; Kock, Patricia; Bezemer, Rex; Weisbeek, Peter; Smeekens, Sjef

doi:10.1104/pp.110.2.455

Cited by 64 publications

(44 citation statements)

References 35 publications

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“…The steady state RNA levels of PC and several additional genes were analyzed ( Figure 1). No PC mRNA was detected in wild-type PC-LUC seedlings grown on a medium with 3% sucrose, in agreement with previous results (Dijkwel et al, 1996). All of the mutants The sun mutants were crossed, and the F, generation of the crosses was sown on a medium with 3% sucrose.…”

Section: Sucrose-uncoupled Mrna Accumulationsupporting

confidence: 78%

“…The plastocyanin-luciferase (PC-LUC) construct was assembled and transferred into Arabidopsis thaliana C24, as described in Dijkwel et al (1996). The PC-LUC transgenic plant line pclA2.7 (wild-type PC-LUC) has been described elsewhere (Dijkwel et al, 1996).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…The expression of the Arabidopsis PC gene has been studied in some detail (Vorst et al, 1988(Vorst et al, , 1993Fisscher et al, 1994Fisscher et al, , 1996Dijkwel et al, 1996). The expression of this gene can be induced by phytochrome through modulation of its promoter activity (P.P.…”

Section: Introductionmentioning

confidence: 99%

“…FR-HIRs were repressed by sucrose in the wild type but were uncoupled from sucrose repression in a subset of the mutants, suggesting that there is a close interaction between metabolic repression and light signaling. (Dijkwel et al, 1996). To obtain more insight into the signal transduction pathways that regulate sucrosedependent repression of PC gene expression, we designed a screen to isolate mutants altered in this process.…”

Section: Introductionmentioning

confidence: 99%

“…(wild-type PC-LUC; Dijkwel et al, 1996) were treated with ethylmethane sulfonate (EMS), as described in Methods. M2-mutagenized seed was collected from 49 independent batches of M, plants.…”

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confidence: 99%

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Sucrose control of phytochrome A signaling in Arabidopsis.

et al. 1997

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The expression of the Arabidopsis plastocyanin (PC) gene is developmentally controlled and regulated by light. During seedling development, PC gene expression is transiently induced, and this induction can be repressed by sucrose. In transgenic seedlings carrying a PC promoter-luciferase fusion gene, the luciferase-induced in vivo luminescence was similarly repressed by sucrose. From a mutagenized population of such transgenic seedlings, we selected for mutant seedlings that displayed a high luminescence leve1 when grown on a medium with 3% sucrose. This screening of mutants resulted in the isolation of several sucrose-uncoupled (sun) mutants showing reduced repression of luminescence by sucrose. Analysis of the sun mutants revealed that the accumulation of PC and chlorophyll a/b binding protein (CAB) mRNA was also sucrose uncoupled, although the extent of uncoupling varied. The effect of sucrose on far-red light high-irradiance responses was studied in wild-type, sunl, sun6, and sun7 seedlings. In wild-type seedlings, sucrose repressed the far-red light-induced cotyledon opening and inhibition of hypocotyl elongation. sun7 seedlings showed reduced repression of these responses. Sucrose also repressed the far-red light-induced block of greening in wild-type seedlings, and both sun6 and sun7 were affected in this response. The results provide evidence for a close interaction between sucrose and light signaling pathways. Moreover, the sun6 and sun7 mutants genetically identify separate branches of phytochrome A-dependent signal transduction pathways.

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Section: Sucrose-uncoupled Mrna Accumulationsupporting

confidence: 78%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%