Sucrose regulation of ADP-glucose pyrophosphorylase subunit genes transcript levels in leaves and fruits

Li, Xiangyang; Xing, Jinpeng; Gianfagna, Thomas J.; Janes, Harry W.

doi:10.1016/s0168-9452(01)00565-9

Cited by 45 publications

(40 citation statements)

References 22 publications

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“…Coordinated sugar transcriptional regulation of several starch biosynthetic genes has been suggested by numerous works (19). Induction of several ADP-Glc PPase-encoding genes has been reported in different species including Arabidopsis (16,(25)(26)(27)(28). We show that, under physiological conditions, only two ADP-Glc PPaseencoding genes, ApL3 and ApL4, are controlled by sugars (Figs.…”

Section: Discussionsupporting

confidence: 66%

Differential Pattern of Expression and Sugar Regulation of Arabidopsis thaliana ADP-glucose Pyrophosphorylase-encoding Genes

Crevillén

Ventriglia

Pinto

et al. 2005

Journal of Biological Chemistry

106

104

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ADP-glucose pyrophoshorylase (ADP-Glc PPase) catalyzes the first and limiting step in starch biosynthesis. In plants, the enzyme is composed of two types of subunits (small and large) and is allosterically regulated by 3-phosphoglycerate and phosphate. The pattern of expression and sugar regulation of the six Arabidopsis thaliana ADP-Glc PPase-encoding genes (two small subunits, ApS1 and ApS2; and four large subunits, ApL1-ApL4) has been studied. Based on mRNA expression, ApS1 is the main small subunit or catalytic isoform responsible for ADP-Glc PPase activity in all tissues of the plant. Large subunits play a regulatory role, and the data presented define a clear functional distinction among them. ApL1 is the main large subunit in source tissues, whereas ApL3 and, to a lesser extent, ApL4 are the main isoforms present in sink tissues. Thus, in source tissues, ADP-Glc PPase would be finely regulated by the 3-phosphoglycerate/phosphate ratio, whereas in sink tissues, the enzyme would be dependent on the availability of substrates for starch synthesis. Sugar regulation of ADP-Glc PPase genes is restricted to ApL3 and ApL4 in leaves. Sugar induction of ApL3 and ApL4 transcription in leaves allows the establishment of heterotetramers less sensitive to the allosteric effectors, resembling the situation in sink tissues. The results presented on the expression pattern and sugar regulation allow us to propose a gene evolution model for the Arabidopsis ADP-Glc PPase gene family.

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Section: Discussionsupporting

confidence: 66%

Differential Pattern of Expression and Sugar Regulation of Arabidopsis thaliana ADP-glucose Pyrophosphorylase-encoding Genes

Crevillén

Ventriglia

Pinto

et al. 2005

Journal of Biological Chemistry

106

104

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“…However, in contrast to the results of Li et al (2002), who showed that the expression of both AgpL1 and AgpS1 (referred to as AgpS1 and AgpB, respectively) were induced in fruit by sucrose at the transcriptional level, a clear response was not observed in our study (Yin et al 2010). At present, there is less information regarding the regulation of the gene for the small subunit than those for the large subunits.…”

contrasting

confidence: 99%

“…However, a proline-responsive cis-element was not found in the promoter sequence. It was reported that a 3,178 bp promoter sequence of AgpL1 encoding the AGPase large subunit has at least four possible cis-elements responsive to sugar (Li et al 2002). Based on sequence comparison, there is little similarity between the promoters of AgpS1 and AgpL1.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that plant AGPase genes are regulated at the transcriptional level by phosphate, nitrate and sugars (Li et al 2002;Müller-Röber et al 1990;Nielsen et al 1998;Scheible et al 1997;Sokolov et al 1998). Additionally, our previous research revealed that AgpS1 and AgpL1 were specifically upregulated at the transcriptional level by salinity stress in early developing fruits in an ABA-and osmotic stressindependent manner (Yin et al 2010).…”

mentioning

confidence: 91%

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Isolation and characterisation of the ADP-glucose pyrophosphorylase small subunit gene (AgpS1) promoter in tomato (Solanum lycopersicum L.)

Goto

Nonaka

Yin

et al. 2013

Plant Biotechnology

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ADP-glucose pyrophosphorylase (AGPase) is a key regulatory enzyme in starch biosynthesis. In this research, 2,885 bp of the predicted promoter sequence for the AgpS1 gene encoding the AGPase small subunit was isolated from tomato. Sequence analyses revealed a number of known cis-elements related to responses to salt and dehydration stress and sugar repression; predicted TATA boxes are located at −88 to −94 bp and −114 to −120 bp. The spatial expression pattern and tissue/organ specificity of AgpS1 were analysed in during development using promoter-GUS transgenic tomato plants. Based on GUS staining, the obtained sequence was proven to be the functional promoter and directed broad expression in both sink and source tissues/organs, including seedling, stem, flower, fruit stalk, fruit and root. In source leaf and early developing fruit, GUS staining was observed in all tissues, except for epidermal tissue. In contrast, GUS staining tended to be confined to vascular tissues in seedling, stem, fruit stalk and ripening fruit. In particular, a patchy staining pattern was observed in the phloem of the stem and fruit stalk, suggesting that AgpS1 is expressed in the phloem companion cells in those organs. These results also suggest that AGPase mainly functions in the vascular tissue of those organs.

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“…It has been shown that Arabidopsis APL3 is restricted to the starch sheath layer below the major veins in leaves and petioles and that its expression is strongly induced by sucrose or trehalose in the mesophyl of the leaf (33,47). Induction by sucrose has also been reported for the tomato and potato LS1 genes (48,49) that are closely related to Arabidopsis APL3 (Fig. 3).…”

Section: Fig 2 Activation By 3-pga Of the Recombinant Arabidopsis Amentioning

confidence: 72%

The Different Large Subunit Isoforms of Arabidopsis thaliana ADP-glucose Pyrophosphorylase Confer Distinct Kinetic and Regulatory Properties to the Heterotetrameric Enzyme

Crevillén

Ballícora

Mérida

et al. 2003

Journal of Biological Chemistry

126

153

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ADP-glucose pyrophosphorylase catalyzes the first and limiting step in starch biosynthesis and is allosterically regulated by the levels of 3-phosphoglycerate and phosphate in plants. ADP-glucose pyrophosphorylases from plants are heterotetramers composed of two types of subunits (small and large). In this study, the six Arabidopsis thaliana genes coding for ADP-glucose pyrophosphorylase isoforms (two small and four large subunits) have been cloned and expressed in an Escherichia coli mutant deficient in ADP-glucose pyrophosphorylase activity. The co-expression of the small subunit APS1 with the different Arabidopsis large subunits (APL1, APL2, APL3, and APL4) resulted in heterotetramers with different regulatory and kinetic properties. Heterotetramers composed of APS1 and APL1 showed the highest sensitivity to the allosteric effectors as well as the highest apparent affinity for the substrates (glucose-1-phosphate and ATP), whereas heterotetramers formed by APS1 and APL2 showed the lower response to allosteric effectors and the lower affinity for the substrates. No activity was detected for the second gene coding for a small subunit isoform (APS2) annotated in the Arabidopsis genome. This lack of activity is possibly due to the absence of essential amino acids involved in catalysis and/or in the binding of glucose-1-phosphate and 3-phosphoglycerate. Kinetic and regulatory properties of the different heterotetramers, together with sequence analysis has allowed us to make a distinction between sink and source enzymes, because the combination of different large subunits would provide a high plasticity to ADP-glucose pyrophosphorylase activity and regulation. This is the first experimental data concerning the role that all the ADP-glucose pyrophosphorylase isoforms play in a single plant species. This phenomenon could have an important role in vivo, because different large subunits would confer distinct regulatory properties to ADP-glucose pyrophosphorylase according to the necessities for starch synthesis in a given tissue.

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Sucrose regulation of ADP-glucose pyrophosphorylase subunit genes transcript levels in leaves and fruits

Cited by 45 publications

References 22 publications

Differential Pattern of Expression and Sugar Regulation of Arabidopsis thaliana ADP-glucose Pyrophosphorylase-encoding Genes

Differential Pattern of Expression and Sugar Regulation of Arabidopsis thaliana ADP-glucose Pyrophosphorylase-encoding Genes

Isolation and characterisation of the ADP-glucose pyrophosphorylase small subunit gene (AgpS1) promoter in tomato (Solanum lycopersicum L.)

The Different Large Subunit Isoforms of Arabidopsis thaliana ADP-glucose Pyrophosphorylase Confer Distinct Kinetic and Regulatory Properties to the Heterotetrameric Enzyme

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