Sucrose Preference and Novelty-Induced Hypophagia Tests in Rats using an Automated Food Intake Monitoring System

Schalla, Martha A.; Kühne, Stephanie Gladys; Friedrich, Tiemo; Hanel, Vivien; Kobelt, Peter; Goebel-Stengel, Miriam; Rose, Matthias; Stengel, Andreas

doi:10.3791/60953

Cited by 5 publications

(7 citation statements)

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“…We incorporated several features into the basic NSF paradigm to permit reliable and meaningful assessments of caloric intake. We trained mice to eat under the test conditions for several days until baseline levels of intake stabilized and excluded those that failed to train ( Schalla et al, 2020 ). We calculated intake from the first bite and not the start of the test.…”

Section: Discussionmentioning

confidence: 99%

“…This is critical, as the high novel cage latencies (≥15 min) exhibited by most of the female groups and the overweight males would confound interpretation of feeding measurements that started at the onset of the test. We evaluated intake for 30 min, as recommended ( Schalla et al, 2020 ; Ellacott et al, 2010 ). Finally, analyzing both baseline and stress-induced conditions makes it possible to parse the effects of acute stress from those imposed by stressors built into the paradigm.…”

Section: Discussionmentioning

confidence: 99%

“…This could stem from a lower appetitive drive in the home test compared to an overnight fast. Diurnal influences, and not prandial state, are also more important influences on food intake in rats ( Schalla et al, 2020 ). The high degree of variability in food intake responses in the dark cycle in lean females and males is similar to what is seen in humans ( Wardle et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

“…To minimize stressors associated with manual measurements, such as cage transport, lid opening, and human interaction, we adapted the assay to an automated food intake monitoring system (BioDAQ, Research Diets) ( Schalla et al, 2020 ). Mice were acclimated to the BioDAQ cages and food hoppers for 5–7 days.…”

Section: Methodsmentioning

confidence: 99%

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Assessing the effects of stress on feeding behaviors in laboratory mice

François

Delgado

Shargorodsky

et al. 2022

eLife

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Stress often affects eating behaviors, increasing caloric intake in some individuals and decreasing it in others. The determinants of feeding responses to stress are unknown, in part because this issue is rarely studied in rodents. We focused our efforts on the novelty-suppressed feeding (NSF) assay, which uses latency to eat as readout of anxiety-like behavior, but rarely assesses feeding per se. We explored how key variables in experimental paradigms – estrous and diurnal cyclicity, age and duration of social isolation, prandial state, diet palatability, and elevated body weight – influence stress-induced anxiety-like behavior and food intake in male and female C57BL/6J mice. Latency to eat in the novel environment is increased in both sexes across most of the conditions tested, while effects on caloric intake are variable. In the common NSF assay (i.e., lean mice in the light cycle), sex-specific effects of the length of social isolation, and not estrous cyclicity, are the main source of variability. Under conditions that are more physiologically relevant for humans (i.e., overweight mice in the active phase), the novel stress now elicits robust hyperphagia in both sexes . This novel model of stress eating can be used to identify underlying neuroendocrine and neuronal substrates. Moreover, these studies can serve as a framework to integrate cross-disciplinary studies of anxiety and feeding related behaviors in rodents.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Assessing the effects of stress on feeding behaviors in laboratory mice

François

Delgado

Shargorodsky

et al. 2022

eLife

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“…Because some of the assays were designed to study anxiety-like behavior, the standard reported measures of food intake are not adequate to draw meaningful conclusion about appetite drive (57). In assays involving intake of a palatable food/drink, rodents are typically acclimated to the novel substance for a few days, without confirming that they reached a stable baseline (138). If the test involves consumption of the diet in a manner to which the rodent is not accustomed (i.e., in a weight boat or pipette), they should be trained for 5-7 days beforehand.…”

Section: Technical Considerations In Measuring Feeding Behaviorsmentioning

confidence: 99%

A Framework for Developing Translationally Relevant Animal Models of Stress-Induced Changes in Eating Behavior

François¹,

Fernández-Gayol²,

Zeltser³

2022

Biological Psychiatry

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Sex-dependent effect of inflammatory pain on negative affective states is prevented by kappa opioid receptors blockade in the nucleus accumbens shell

Lorente

Cuitavi

Rullo

et al. 2023

Preprint

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Background and purpose: Psychological disorders, such as anxiety and anhedonia are pain comorbidities, however how pain affects male and female individuals and through which mechanism is not well understood. Previous research show pain-induced alterations in the dynorphinergic pathway in the mesocorticolimbic system (MCLS) together with a relationship between corticotropin-releasing system and dynorphin release in the MCLS. Here, we analyse the sex and time course-dependent effects of pain on negative affect. Additionally, we study the implication of dynorphinergic and corticotropin releasing factor involvement in these pain related behaviours. Experimental approach: We used behavioural pharmacology and biochemical tools to characterize negative affective states induced by inflammatory pain in male and female rats, and the alterations in dynorphinergic and corticotropin systems in the MCLS. Key results: Female rats showed a persistent anxiety-like together with a reversible anhedonia-like behaviours derived from inflammatory pain. Additionally, we found alterations of in both dynorphin and corticotropin releasing factor in NAc and amygdala that suggest sex-dependent dynamic adaptations. Finally blockade on the kappa opioid receptor in the NAc confirmed its role in pain-induced anxiety-like behaviour in female rats. Conclusions and implications: Our results show sex and time dependent anxiety- and anhedonia-like behaviours induced by the presence of pain in female rats. Furthermore, we replicated previous data pointing to the KOR/dyn recruitment in the NAc as key neurological substrate mediating these behaviours. This research encourages the study the mechanisms underlying these behaviours, to better understand the emotional dimension of pain.

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Sucrose Preference and Novelty-Induced Hypophagia Tests in Rats using an Automated Food Intake Monitoring System

Cited by 5 publications

References 0 publications

Assessing the effects of stress on feeding behaviors in laboratory mice

Assessing the effects of stress on feeding behaviors in laboratory mice

A Framework for Developing Translationally Relevant Animal Models of Stress-Induced Changes in Eating Behavior

Sex-dependent effect of inflammatory pain on negative affective states is prevented by kappa opioid receptors blockade in the nucleus accumbens shell

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