Sucrose Nephrosis: Electron Microscopic and Histochemical Observations

Janigan, David T.; Santamaría, Armando; Trump, Benjamin F.

doi:10.1177/8.5.385

Cited by 15 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has long been known that hypertonic sucrose produces vacuolization of hepatic and renal cells (11,12). More recently these vacuoles have been shown to be lysosomal in nature (13), to contain labeled sucrose (14), while their formation is blocked by the prior uptake of invertase (15).…”

Section: Discussionmentioning

confidence: 99%

The Uptake, Storage, and Intracellular Hydrolysis of Carbohydrates by Macrophages

Cohn

Ehrenreich

1969

The Journal of Experimental Medicine

184

View full text Add to dashboard Cite

Recent studies have outlined some of the relationships which exist between various members of the vacuolar system of macrophages (1)(2)(3)(4)(5). In response to environmental stimuli, plasma membrane is interiorized as a pinocytic vesicle which then migrates to the peri-Golgi zone of the cytoplasm. Here it acquires lysosomal enzymes, presumably by way of Golgi vesicles, and is converted into a secondary lysosome or digestive body. This is the locus for the intracellular digestion of exogenous molecules, a process which in the case of proteins may be to the level of amino acids (6, 7).Many questions concerning the macrophage lysosome remain to be answered. These include the permeability of its limiting membrane, the diversity of its hydrolytic enzymes, the fate of nondigestible molecules stored within it, and the role of extraceUular hydrolases taken up by pinocytosis. Certain of these problems have been approached under in vitro conditions and through the use of selected digestible and nondigestible molecules. This article will deal with the mechanism of uptake, storage, and hydrolysis of oligosaccharides added to the culture medium of mouse peritoneal macrophages. Materials and MetkodsMacrophage Cultures.--Leighton tubes: Morphological studies were conducted with unstimulated mouse peritoneal macrophages cultivated on flying cover slips in medium 199 containing varying concentrations of newborn calf serum (NBCS). Components of the medium were obtained from either Microbiological Associates (Bethesda, Md.) or Grand Island Biologicals (Grand Island, N.Y.). The details of this procedure have been reported previously (1). Additions to the medium will be described under Results.T flask cultures: Mass cultures of homogeneous macrophage populations were prepared in 30 cm 2 T flasks and incubated in medium 199 containing NBCS. Cell suspensions were obtained immediately after the removal of lymphocytes at time zero (To) or after 24--48 hr of incubation at 37°C. The preparation and harvesting of cells have been described (1).Fixation, Microscopy, and Pkotograplty--Cells maintained on cover slips were fixed in the

show abstract

Section: Discussionmentioning

confidence: 99%

The Uptake, Storage, and Intracellular Hydrolysis of Carbohydrates by Macrophages

Cohn

Ehrenreich

1969

The Journal of Experimental Medicine

184

View full text Add to dashboard Cite

show abstract

“…158 greatly enlarged lysosomes (Brewer & Heath, 1963;Rohr & Zollinger, 1966). The increased size of the lysosomes is possibly due to an influx of water to maintain the osmotic balance between the interior of the lysosome and the cytosol (Wattiaux, 1966), and leakage of acid hydrolases from the enlarged lysosomes results in liver damage (Janigan et al, 1960). Other cell types in which the effects of sucrose have been studied include cultured chick-embryo skeletal tissue (Dingle et al, 1969), mouse peritoneal macrophages (Cohn & Ehrenreich, 1969), mouse leukaemic cells (Bernacki & Bosmann, 1971) and Chinesehamster ovary fibroblasts (Munro, 1968).…”

mentioning

confidence: 99%

The effect of intralysosomal sucrose storage on the turnover of hamster fibroblast lysosomal and Golgi-apparatus enzymes

Warburton¹,

Wynn²

1976

Biochemical Journal

View full text Add to dashboard Cite

1. The effect of overloading of hamster fibroblast lysosomes with sucrose on the turnover of lysosomal and Golgi-apparatus enzymes was studied. Arylsulphatase B and UDP-galactose-N-acetylglucosamine galactosyltransferase were chosen as appropriate marker enzymes. The relative contributions of changes in the rates of synthesis and degradation to the increased activities of these enzymes after uptake of sucrose were examined by isotopic-labelling experiments. The effects of sucrose uptake on the degradation of total cellular protein and of the cytoplasmic enzyme, alkaline ribonuclease, were determined for comparative purposes. 2. The rates of enzyme synthesis in the presence and absence of sucrose were compared by pulse-labelling the cells with 14C-labelled amino acids, followed by isolation of purified enzymes and determination of their radioactivity. Sucrose uptake produced increases of 270% and 90% respectively in the rates of synthesis of arylsulphatase B and galactosyltransferase, whereas the rate of synthesis of alkaline ribonuclease was not affected. 3. The rates of degration of the enzymes were estimated by measuring the decay with time of the radioactivity of purified enzymes from prelabelled cells. In the absence of sucrose, the apparent half-lives of arylsulphatase B and galactosyltransferase were about 30 days and 40h respectively. After uptake of sucrose, the half-life of arylsulphatase B decreased to about 10 days and that of galactosyltransferase to 10h. Neither the half-life of alkaline ribonuclease (4 days) nor the rate of degradation of total cellular protein was affected by the uptake of sucrose. 4. These results indicate that the hyperactivity of lysosmal and Golgi-apparatus enzymes after the uptake of sucrose is accompanied by increases in the rates of both synthesis and degradation, and that the increased rates of degradation are insufficient to prevent accumulation of the excess of enzymes synthesized; also that the effects of sucrose uptake are restricted to the vacuolar apparatus.

show abstract

“…The only difference observed between functioning and oliguric homotransplants, apart from the degree of cellular infiltration, was a striking intracellular redistribution of acid phosphatase, demonstrated histochemically. The alteration in the localization of this enzyme is not a specific characteristic of a rejecting homotransplanted kidney, but is an early indication of cell damage (Novikoff, 1959;Becker and Barron, 1961;Janigan and Santamaria, 1961). The quantitative changes in enzyme levels observed in the longer-surviving homotransplants can be explained by the massive cellular infiltration.…”

Section: Chemical Analysesmentioning

confidence: 99%

Some Biochemical Changes in the Transplanted Kidney

Williams¹,

Tyler²,

Morton³

et al. 1962

BMJ

View full text Add to dashboard Cite

Sucrose Nephrosis: Electron Microscopic and Histochemical Observations

Abstract: Letters to the Editor 385 SU('l)Sl' N EPHR()SIS: l'IAl'X'TR()N MICI)SC()PI(' ANI) HISTOCHI#{176}\I ICAL OBSERVATIONS I)c I)ouvc limos n'oo'enmt lv dis('uisse(l I lie mole of lvsolsolnio-s i mu i rot n'muchlomlmun' oligest i ye lln'olo'oss(-s

Cited by 15 publications

References 0 publications

The Uptake, Storage, and Intracellular Hydrolysis of Carbohydrates by Macrophages

The Uptake, Storage, and Intracellular Hydrolysis of Carbohydrates by Macrophages

The effect of intralysosomal sucrose storage on the turnover of hamster fibroblast lysosomal and Golgi-apparatus enzymes

Some Biochemical Changes in the Transplanted Kidney

Contact Info

Product

Resources

About