Sucrose metabolism in sugar cane grown under varying climatic conditions: Synthesis and storage of sucrose in relation to the activities of sucrose synthase, sucrose-phosphate synthase and invertase

Batta, S. K.; Singh, Rangil

doi:10.1016/s0031-9422(00)84484-2

Cited by 80 publications

(43 citation statements)

References 26 publications

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“…lB), those internodes were selected for carbon partitioning and metabolite analysis. Since both SUC (Lingle, 1989) and the two reducing monomers, Glc and Fru (Sacher et al, 1963;Glasziou and Gaylor, 1972;Batta and Singh, 1986), can be taken up by sugarcane culm tissue, the extent of carbon partitioning in the sink tissues was investigated by independently supplying tissue slices with a11 three of these sugars uniformly labeled.…”

Section: Carbon Partitioningmentioning

confidence: 99%

“…Knowledge about carbon partitioning between different cellular constituents in sugarcane culm tissues is limited to partitioning within the sugar pool (Sacher et al, 1963;Batta and Singh, 1986;Lingle, 1989). In sugarcane cellsuspension cultures, increased carbon partitioning into SUC is at the expense of both respiration and structural material under nitrogen-limiting conditions (Veith and Komor, 1993).…”

mentioning

confidence: 99%

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Carbon Partitioning during Sucrose Accumulation in Sugarcane Internodal Tissue

1997

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l h e temporal relationship between sucrose (Suc) accumulation and carbon partitioning was investigated in developing sugarcane internodes. Radiolabeling studies on tissue slices, which contained Suc concentrations ranging from 14 to 42% of the dry mass, indicated that maturation coincided with a redirection of carbon from water-insoluble matter, respiration, amino acids, organic acids, and phosphorylated intermediates into SUC. It is evident that a cycle of Suc synthesis and degradation exists in all of the internodes. l h e decreased allocation of carbon to respiration coincides with a decreased flux from the hexose pool. 60th the glucose and fructose (Fru) concentrations significantly decrease during maturation. The phosphoenolpyruvate, Fru-6-phosphate (Fru-6-P), and Fru-2,6-bisphosphate (Fru-2, 6-P,) concentrations decrease between the young and older internodal tissue, whereas the inorganic phosphate concentration increases. lhe calculated mass-action ratios indicate that the ATP-dependent phosphofructokinase, pyruvate kinase, and Fru-l,6-bisphosphatase reactions are tightly regulated in all of the internodes, and no evidence was found that major changes in the regulation of any of these enzymes occur. l h e pyrophosphatedependent phosphofructokinase reaction is in apparent equilibrium in ali the internodes. Substrate availability might be one of the prime factors contributing to the observed decrease in respiration.

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Section: Carbon Partitioningmentioning

confidence: 99%

mentioning

confidence: 99%

Carbon Partitioning during Sucrose Accumulation in Sugarcane Internodal Tissue

1997

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“…Sugarcane storage tissue accumulates sugar against a concentration gradient using energy provided by respiration (Burg and Bieleski 1962). This is accompanied by a continuous cleavage and synthesis of sucrose during accumulation of sucrose in storage tissue (Batta and Singh 1986). Primary sucrose metabolism is governed by several enzymes (Quick and Schaffer 1996).…”

Section: Introductionmentioning

confidence: 99%

Transcript expression and soluble acid invertase activity during sucrose accumulation in sugarcane

et al. 2011

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Soluble acid invertase (SAI, EC 3.2.1.26), catalyzes the hydrolysis of sucrose into hexose sugars, and it has been considered a key enzyme for carbohydrate metabolism. In the present study, the activity of SAI enzyme was determined to establish a correlation between the change in transcript levels and enzyme activity in high and low sugar accumulating sugarcane cultivars, in various internodal tissues at different developmental stages. A decrease in SAI activity and transcript levels was observed with age, during all the developmental stages in both the cultivars. A negative correlation between SAI activity and sucrose content was observed in mature and immature internodes; however, there was a positive correlation between SAI activity and content of hexose sugars. These results imply that SAI plays a crucial role in sucrose partitioning in various intermodal tissues in high and low sugar cultivars. In addition to this, the changes in enzyme activity also resulted in changes in transcript level.

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“…The activities of SAI and NI were measured according to Batta and Singh (1986) and the absorbance was determined at 520 nm. The protein content in the enzyme extract was measured according to Bradford (1976).…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, we examined whether glucose application can protect membranes of chloroplasts and improve cucumber growth under heat stress due to increased antioxidant enzyme activities. It has been reported that proline (Zhang et al 2014) and soluble sugars (Santarius 1973) are able to mitigate stress, and soluble acid invertase (SAI) and neutral invertase (NI) are important enzymes in sugar metabolism in plants (Batta and Singh 1986). Thus, we also investigated whether proline, soluble sugars, SAI, and NI are involved in heat stress mitigation after glucose pretreatment.…”

Section: Introductionmentioning

confidence: 97%

Glucose application protects chloroplast ultrastructure in heat-stressed cucumber leaves through modifying antioxidant enzyme activity

Huang¹,

Zhou²,

Yang³

et al. 2015

Biologia plant.

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To elucidate a physiological mechanism of heat stress mitigation by exogenous glucose, seedlings of Cucumis sativus cv. Jinchun No. 4 were pretreated with glucose and then exposed to normal (25/18 °C) and elevated (42/38 °C) temperatures. We investigated whether glucose can protect cucumber plantlets and chloroplast ultrastructure from heat and whether this protection is associated with antioxidant enzymes, proline, and soluble sugars. Heat inhibited plant growth, disorganized membranes of 86.33 % of chloroplasts, and elevated the content of malondialdehyde (MDA), superoxide radical (O 2 .-), and hydrogen peroxide (H 2 O 2 ). An optimum concentration of glucose was 30 mM as it significantly alleviated plant growth inhibition and obviously reduced the content of MDA, O 2 .-, and H 2 O 2 under the heat stress. The pretreatment with 30 mM glucose mitigated heat-induced damage of chloroplast ultrastructure and changes in leaf morphology more than 30 mM mannitol suggesting that glucose did not act only as osmolyte. Moreover, the glucose pretreatment increased activities of some antioxidant enzymes and enhanced the content of proline and soluble sugars under the heat stress, as well as the transcriptions of Cu/Zn-superoxide dismutase, Mn-superoxide dismutase, catalase, and glutathione reductase genes. We conclude that the pretreatment with 30 mM glucose protected chloroplast ultrastructure and enhanced heat tolerance of the seedlings by the increased activites of antioxidants and the content of proline and soluble sugars, and the repressed accumulation of reactive oxygen species.

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Sucrose metabolism in sugar cane grown under varying climatic conditions: Synthesis and storage of sucrose in relation to the activities of sucrose synthase, sucrose-phosphate synthase and invertase

Cited by 80 publications

References 26 publications

Carbon Partitioning during Sucrose Accumulation in Sugarcane Internodal Tissue

Carbon Partitioning during Sucrose Accumulation in Sugarcane Internodal Tissue

Transcript expression and soluble acid invertase activity during sucrose accumulation in sugarcane

Glucose application protects chloroplast ultrastructure in heat-stressed cucumber leaves through modifying antioxidant enzyme activity

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