Sucrose Metabolism in Bean Plants Under Water Deficit

Castrillo, M.

doi:10.1093/jxb/43.12.1557

Cited by 44 publications

(34 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4). Increased CIN activity promoted by osmotic stress has been reported in rice , pea (Castrillo 1992), and sweet potato (Wang et al 1999). The expression of OsSUT2 was induced by wounding and sucrose treatment (Aoki et al 2003).…”

Section: Discussionmentioning

confidence: 95%

Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation

Lee

Huang

2013

Plant Growth Regul

View full text Add to dashboard Cite

This study aimed to clarify the possible mechanism of endogenous phytohormone signaling and carbohydrate metabolism during shoot organogenesis induced by osmotic stress in rice (Oryza sativa L. cv. Tainung 71) callus. Non-regenerable calli derived from Tainung 71 immature embryos were inoculated on Murashige and Skoog medium containing 10 lM 2,4-D. They turned to highly regenerable calli (HRC) (regeneration frequency more than 75 %) with lower calli fresh weight and water content when 0.6 M sorbitol was supplemented into the medium. The regeneration frequency was prominently decreased to 25 % while an auxin transport inhibitor, 2,3,5-triiodobenzoic acid (TIBA), was added into the sorbitoltreated medium. It suggested that endogenous auxin signal may be involved in the induction of HRC under osmotic stress treatment. As well, HRC showed high levels of glucose, sucrose, and starch and high expression of cell wall-bound invertase 1, sucrose transporter 1 (OsSUT1), OsSUT2, PIN-formed 1, and late embryogenesis abundant 1 (OsLEA1) genes. Their expressions are all dramatic inhibited except OsLEA1 under TIBA treatment. It suggests a key role of auxin may be linked to the effect of shoot regeneration under osmotic stress treatment. Therefore, we present a putative hypothesis for regenerable calli induction by osmotic stress treatment in rice. Osmotic stress may regulate endogenous levels of auxin interacting with abscisic acid, then affect carbohydrate metabolism to trigger callus initiation and further shoot regeneration in rice.

show abstract

Section: Discussionmentioning

confidence: 95%

Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation

Lee

Huang

2013

Plant Growth Regul

View full text Add to dashboard Cite

show abstract

“…4b, d) was negative for most of the water stress period. The accumulation of soluble sugars during day two of water shortage was probably due to the arrest in foliar growth, a process which is more sensitive with regard to water deficit than photosynthesis (Hsiao, 1973;Boyer, 1976) and sucrose synthase activity (Castrillo, 1992). From stress day four onward, the soluble sugar content at 18:00 h was significantly reduced, especially for Carioca, indicating a significant reduction in A during these days, reducing the soluble sugar daily balance to negative values (Fig.…”

Section: Discussionmentioning

confidence: 96%

“…Many researchers have investigated the relation between CO 2 assimilation and end-products in bean (Castrillo, 1992) and others species (Quick et al, 1992;Farrar et al, 2000;Wingler et al, 2006), showing the important role of leaf soluble sugar amounts in the feedback regulatory mechanisms of photosynthesis. In addition, starch and amino acid biosynthesis, which are regulated by soluble sugar amounts, can also act as a secondary feedback regulatory mechanism of photosynthesis (Paul and Pellny, 2003).…”

Section: Introductionmentioning

confidence: 99%

Daily balance of leaf sugars and amino acids as indicators of common bean (Phaseolus vulgaris L.) metabolic response and drought intensity

Santos

Pimentel

2009

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Leaf soluble sugar, starch and free amino acid amounts were evaluated in two bean genotypes, Carioca and Ouro Negro, grown in 10 L pots in a greenhouse. This was realized during a single day for Carioca and during ten days of water deficit for both genotypes, at 06:00 and 18:00 h. During the day, an increase in all parameters occurred up to midday, while in the afternoon, carbohydrate amounts varied in opposition to amino acids amounts. Under water deficit, the leaf soluble sugars at 18:00 and their daily balance (amount at 18:00 minus amount at 06:00) increased on the 2 nd day, with starch and amino acid amounts and their respective daily balances decreasing. On the 8 th and 10 th days, the soluble sugar daily balance was negative and the starch daily balance approached zero or was negative, while the amino acid daily balance was positive. During rehydration, soluble sugar daily balance was close to zero and starch daily balance increased, whereas amino acid daily balance was negative. The carbohydrate daily balance, strongly related to free amino acid daily balance, were good indicators for evaluating the stage of water deficit and metabolic capacity to respond to the stress in common bean.

show abstract

“…In other plant species, anoxia decreased Sus activity and protein content in soybean nodules [27], but increased Sus4 mRNA in potato [28] and in soybean callus tissue [29]. Other abiotic stresses have also been reported to affect the level of Sus protein or activity ; for example, water stress induced Sus protein in Populus leaves [17] and increased Sus activity in bean leaves [30].…”

Section: Abiotic Stresses Trigger the Differential Expression Of Sus mentioning

confidence: 99%

Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis

Déjardin

Sokolov

Kleczkowski

1999

Biochemical Journal

111

View full text Add to dashboard Cite

Sucrose synthase (Sus) is a key enzyme of sucrose metabolism. Two Sus-encoding genes (Sus1 and Sus2) from Arabidopsis thaliana were found to be profoundly and differentially regulated in leaves exposed to environmental stresses (cold stress, drought or O2 deficiency). Transcript levels of Sus1 increased on exposure to cold and drought, whereas Sus2 mRNA was induced specifically by O2 deficiency. Both cold and drought exposures induced the accumulation of soluble sugars and caused a decrease in leaf osmotic potential, whereas O2 deficiency was characterized by a nearly complete depletion in sugars. Feeding abscisic acid (ABA) to detached leaves or subjecting Arabidopsis ABA-deficient mutants to cold stress conditions had no effect on the expression profiles of Sus1 or Sus2, whereas feeding metabolizable sugars (sucrose or glucose) or non-metabolizable osmotica [poly(ethylene glycol), sorbitol or mannitol] mimicked the effects of osmotic stress on Sus1 expression in detached leaves. By using various sucrose/mannitol solutions, we demonstrated that Sus1 was up-regulated by a decrease in leaf osmotic potential rather than an increase in sucrose concentration itself. We suggest that Sus1 expression is regulated via an ABA-independent signal transduction pathway that is related to the perception of a decrease in leaf osmotic potential during stresses. In contrast, the expression of Sus2 was independent of sugar/osmoticum effects, suggesting the involvement of a signal transduction mechanism distinct from that regulating Sus1 expression. The differential stress-responsive regulation of Sus genes in leaves might represent part of a general cellular response to the allocation of carbohydrates during acclimation processes.

show abstract

Sucrose Metabolism in Bean Plants Under Water Deficit

Cited by 44 publications

References 17 publications

Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation

Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation

Daily balance of leaf sugars and amino acids as indicators of common bean (Phaseolus vulgaris L.) metabolic response and drought intensity

Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis

Contact Info

Product

Resources

About