Sucrose metabolism during somatic and zygotic embryogeneses in Norway spruce: content of soluble saccharides and localisation of key enzyme activities

Konrádová, Hana; Lipavská, Helena; Albrechtová, Jana; Vreugdenhil, D.

doi:10.1078/0176-1617-00624

Cited by 29 publications

(17 citation statements)

References 33 publications

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“…Interestingly, five Suc-binding proteins showed opposite expression trends, their relative abundance decreased during seed filling (spots 405, 463, 522, 631, and 754). The expression profiles of these proteins may support previous investigations on the importance of Suc as a signaling molecule in seed and embryo development (Konradova et al, 2002;Bate et al, 2004;Yang et al, 2004; for review, see Borisjuk et al, 2004).…”

Section: Multiple Isoforms For Suc-binding Protein and Suc Synthase Ssupporting

confidence: 71%

A Systematic Proteomic Study of Seed Filling in Soybean. Establishment of High-Resolution Two-Dimensional Reference Maps, Expression Profiles, and an Interactive Proteome Database

et al. 2005

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A high-throughput proteomic approach was employed to determine the expression profile and identity of hundreds of proteins during seed filling in soybean (Glycine max) cv Maverick. Soybean seed proteins were analyzed at 2, 3, 4, 5, and 6 weeks after flowering using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. This led to the establishment of high-resolution proteome reference maps, expression profiles of 679 spots, and corresponding matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra for each spot. Database searching with these spectra resulted in the identification of 422 proteins representing 216 nonredundant proteins. These proteins were classified into 14 major functional categories. Proteins involved in metabolism, protein destination and storage, metabolite transport, and disease/defense were the most abundant. For each functional category, a composite expression profile is presented to gain insight into legume seed physiology and the general regulation of proteins associated with each functional class. Using this approach, an overall decrease in metabolism-related proteins versus an increase in proteins associated with destination and storage was observed during seed filling. The accumulation of unknown proteins, sucrose transport and cleavage enzymes, cysteine and methionine biosynthesis enzymes, 14-3-3-like proteins, lipoxygenases, storage proteins, and allergenic proteins during seed filling is also discussed. A user-intuitive database (http://oilseedproteomics.missouri.edu) was developed to access these data for soybean and other oilseeds currently being investigated.

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Section: Multiple Isoforms For Suc-binding Protein and Suc Synthase Ssupporting

confidence: 71%

A Systematic Proteomic Study of Seed Filling in Soybean. Establishment of High-Resolution Two-Dimensional Reference Maps, Expression Profiles, and an Interactive Proteome Database

et al. 2005

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“…A dificuldade na retomada do desenvolvimento desses embriões somáticos foi relacionada a anomalias no meristema apical caulinar e nos cotilédones, características que podem derivar de processos mutagênicos durante a embriogênese somática e também da produção inadequada de carboidratos de reserva (Mendes-da-Glória 1998). Segundo Konrádová et al (2002), as células suspensoras do ápice caulinar são locais com alta atividade enzimática, como de sacarose sintase, metabolizando a sacarose para fornecimento de energia e carbono para as etapas iniciais do desenvolvimento embrionário zigótico. Assim, a inexistência desta etapa na indução e desenvolvimento dos embriões somáticos de A. sellowiana, não estimulando a produção e o acúmulo de teores adequadamente suficientes e/ou tipos específicos de carboidratos nesta fase de desenvolvimento tornariam os explantes inviáveis.…”

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Efeito do frio sobre os carboidratos solúveis em culturas embriogênicas de Acca sellowiana O. Berg (Myrtaceae)

et al. 2009

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-(Effect of cold on soluble carbohydrates of embryogenic cultures of Acca sellowiana O. Berg (Myrtaceae)). The pathway of somatic embryogenesis in Acca sellowiana, a fruit tree species with limitations concerning vegetative propagation, was well established but the number of embryos that restart growth after the cotyledonary phase is low. In this work, somatic embryos at different developmental stages obtained after treatments with 2,4-D were submitted to cold (4 °C) during 20 days to induce transformation of embryos into plantlets. High proportion (95%) of somatic embryogenesis was found from zygotic embryos treated with 2,4-D continuously (T1) when compared to transitory treatment with 2,4-D (T2 -55%). However, T1 induced higher rates (82%) of deformation than T2 (62%). In both treatments, no plantlets were obtained from somatic embryos after cold. In T1, 50% of the embryos cultivated at the globular phase remained quiescent and only embryos at the cotyledonary phase (76%) showed positive responses indicating restart of growth. In T2, only 30% of embryos at cotyledonary phase turned greenish, and presented higher values of total sugars. The carbohydrate analyses showed myo-inositol, glucose, fructose and sucrose as the major components, present in different proportions, the latter decreasing during the culture period. The low levels of raffinose during the growth period and their reduction under cold treatment could be related to changes of the normal somatic embryo development, without accumulation of carbohydrates, thus inhibiting plantlet regeneration.Key words -Acca sellowiana, raffinose, soluble carbohydrates, somatic embryogenesis RESUMO -(Efeito do frio sobre os carboidratos solúveis em culturas embriogênicas de Acca sellowiana O. Berg, (Myrtaceae)). A rota da embriogênese somática de Acca sellowiana, uma espécie arbórea frutífera com limitações quanto à propagação vegetativa, já foi estabelecida mas o número de embriões que restabelecem o crescimento após a fase cotiledonar é baixo. No presente trabalho, embriões somáticos em diferentes fases de desenvolvimento foram obtidos após tratamentos com 2,4-D e submetidos à condição de frio (4 °C) durante 20 dias, visando induzir a transformação de embriões somáticos em plântulas. Observou-se que 95% dos embriões zigóticos desenvolveram embriões somáticos quando tratados com 2,4-D de forma contínua (T1), enquanto que apenas 55% foram formados quando a auxina foi usada na forma pulsada (T2). Porém, o tratamento T1 induziu altas taxas (82%) de deformações nos embriões quando comparados a T2 (62%). Em ambos os tratamentos, não houve formação de plântulas a partir de embriões somáticos após a exposição ao frio, sendo observada redução nos níveis de rafinose. Em T1, 50% dos embriões cultivados na fase globular permaneceram quiescentes e apenas os embriões na fase cotiledonar (76%) apresentaram características positivas de retomada de desenvolvimento, havendo, porém, redução nos teores de açúcares totais com o desenvolvimento e a diferenciação dos embriões....

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“…Marked differences were observed in carbohydrate spectra between developing zygotic and maturing somatic embryos of Picea abies (Gösslová et al 2001). The decrease in total carbohydrate as well as the accumulation of sucrose in later developing stages was a common features in both systems (Konrádová et al 2002). The concentrations of total lipids exhibited marked variation during maturation of Picea abies somatic embryos, indicating the importance of lipid reserves during embryo development (Svobodová et al 1999, Grigová et al 2007).…”

Section: Biochemistry Of Zygotic and Somatic Embryosmentioning

confidence: 98%

Study of Abies somatic embryogenesis and its application

Vooková

2013

Dendrobiology

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This paper provides results of somatic embryogenesis study in our laboratory. General description of somatic embryogenesis (SE) induction, maturation of somatic embryos and plantlets regeneration of the Abies species, followed by a comparisons of some characteristics of zygotic and somatic embryos, seedlings and emblings (somatic seedlings). Own results are supplemented with some literature data. Also aplication of SE for improving of plantlet regeneration of elite fir trees from Dobroč primeval is described as well as initiation of the SE from seeds of incompatible crossings of firs where zygotic embryos abort usually several weeks after pollination.

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Sucrose metabolism during somatic and zygotic embryogeneses in Norway spruce: content of soluble saccharides and localisation of key enzyme activities

Cited by 29 publications

References 33 publications

A Systematic Proteomic Study of Seed Filling in Soybean. Establishment of High-Resolution Two-Dimensional Reference Maps, Expression Profiles, and an Interactive Proteome Database

A Systematic Proteomic Study of Seed Filling in Soybean. Establishment of High-Resolution Two-Dimensional Reference Maps, Expression Profiles, and an Interactive Proteome Database

Efeito do frio sobre os carboidratos solúveis em culturas embriogênicas de Acca sellowiana O. Berg (Myrtaceae)

Study of Abies somatic embryogenesis and its application

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