“…In an in situ study of humans wearing palatal devices containing titanium specimens, daily sucrose exposure was found to increase biofilm biomass and negatively affect the biochemical and microbiological composition of the biofilm formed [ 84 ]. In addition, the adhesion of S. mutans biofilms on titanium substrates was found to be induced by sucrose levels ranging from 0–750 mM [ 85 ]. In particular, S. mutans biofilms formed in the presence of 75 mM sucrose were found to show the maximum adhesion and mounds.…”