Sucrose, maltodextrin and inulin efficacy as cryoprotectant, preservative and prebiotic – towards a freeze dried Lactobacillus plantarum topical probiotic

Oluwatosin, Sumbo Oluwatoyin; Tai, Siew Leng; Fagan-Endres, Marijke A.

doi:10.1016/j.btre.2021.e00696

Cited by 44 publications

(23 citation statements)

References 34 publications

(46 reference statements)

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“…The lyophilization process is the most common way to keep bacteria in an inactivated form to avoid losing colony-forming units (CFU) during a long storage period. This method also enables keeping adequate living probiotics for later use [ 77 , 78 ]. However, this process can damage the structure of probiotic bacteria cells (see Figure 3 ).…”

Section: Resultsmentioning

confidence: 99%

Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics

et al. 2023

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Probiotics have been used in human and veterinary medicine to increase resistance to pathogens and provide protection against external impacts for many years. Pathogens are often transmitted to humans through animal product consumption. Therefore, it is assumed that probiotics protecting animals may also protect the humans who consume them. Many tested strains of probiotic bacteria can be used for individualized therapy. The recently isolated Lactobacillus plantarum R2 Biocenol™ has proven to be preferential in aquaculture, and potential benefits in humans are expected. A simple oral dosage form should be developed to test this hypothesis by a suitable preparation method, i.e., lyophilization, allowing the bacteria to survive longer. Lyophilizates were formed from silicates (Neusilin® NS2N; US2), cellulose derivates (Avicel® PH-101), and saccharides (inulin; saccharose; modified starch® 1500). They were evaluated for their physicochemical properties (pH leachate, moisture content, water absorption, wetting time, DSC tests, densities, and flow properties); their bacterial viability was determined in conditions including relevant studies over 6 months at 4 °C and scanned under an electron microscope. Lyophilizate composed of Neusilin® NS2N and saccharose appeared to be the most advantageous in terms of viability without any significant decrease. Its physicochemical properties are also suitable for capsule encapsulation, subsequent clinical evaluation, and individualized therapy.

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Section: Resultsmentioning

confidence: 99%

Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics

et al. 2023

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“…Maltodextrin has been added as drying aid in drying process of several products such as in infrared drying of avocado pulp [2], freeze drying of Lactobacillus plantarum [10], and spray drying of soybean oil [11]. It was reported that together with sucrose and inulin, maltodextrin act as cryoprotectant, preservative and prebiotic in freeze drying process of L. plantarum, while maltodextrin provide a beneficial in avoiding the reduction of total polyphenols, total chlorophylls, and antioxidant activity as well as minimizing of lipid oxidation in avocado pulp [2], [10].…”

Section: Resultsmentioning

confidence: 99%

Kinetics of Cucurbita Moschata Puree Drying with Maltodextrin as Drying Aid

Hartati

Setiawati

Suwardiyono

et al. 2022

RICE

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The objective of this research was to investigate the influence of maltodextrin addition of pumpkin puree drying as well as studied the drying kinetics. Several thin layer drying kinetics model comprised of Page, Lewis, Henderson Pabis, Two Term, Weibull as well as diffusion model were used to studied the behavior of pumpkin drying. The puree drying was conducted by added 15% of maltodextrin on pumpkin puree. The drying temperatures were varied of 60-70 The research result show that temperature influence the pumpkin puree drying in which added with maltodextrin as the drying aid. Pumpkin puree drying with addition of maltodextrin performed at temperature of 70 for 150 minutes is considered as the relatively suitable condition as it gives low moisture ratio of 0.07. The thin layer drying kinetics, Midili, give good fittest to the experimental data followed by Page and Weibull. The effective diffusivity is in range of m/s with the obtained activation energy is 30.36 kJ/mol.

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“…It has been suggested that these cryoprotectants may serve also as potential prebiotics in topical applications. 19 There are numerous references to the benefits of applying living Lactobacillus to the skin, particularly to troubled skin. Some of the earliest references examined the ability of topically applied Lactobacillus to improve burns.…”

Section: Introductionmentioning

confidence: 99%

“…These low molecular weight oligosaccharides help to maintain the integrity of the microbial cell walls and keep the microorganisms intact and living in the powdered form. It has been suggested that these cryoprotectants may serve also as potential prebiotics in topical applications 19 . There are numerous references to the benefits of applying living Lactobacillus to the skin, particularly to troubled skin.…”

Section: Introductionmentioning

confidence: 99%

Living, quiescent Lactobacillus plantarum Lp90 probiotic, delivered topically to full thickness tissues in vitro via a just‐add‐water cream delivery system, stimulates the expression of elastin protein

Gruber¹,

Holtz

2023

J of Cosmetic Dermatology

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IntroductionDelivering living probiotics to the skin can be challenging as most water‐containing cosmetic products require preservatives to maintain product stability. A recently introduced powdered technology [Stratabiosys™, Vantage Personal Care] allows for quiescent probiotic powders to be stored for extended periods of time. The powders can then be reconstituted to creams at the point of use by adding water and mixing and were examined in vitro on reconstructed human full thickness tissues to see if the probiotic had any influence of several important biomolecules expressed in the skin.Materials and MethodsA probiotic powder containing 200 M CFU/gram of living quiescent Lactobacillus plantarum Lp90 was reconstituted to a cream by adding ultrapure water and gently mixing the components at room temperature to quickly produce a cream. The resulting cream was tested topically on Epiderm® Full Thickness Tissues by treating the tissues for 24 h, removing the cream with a PBS rinse and then repeating the treatment for another 24 h. The resulting tissues were examined for four strategically important skin biomolecules including Type 1A collagen, elastin, filaggrin and hyaluronic acid. The probiotic‐containing powder was tested against untreated tissues and powders not containing probiotics and powders containing measured amounts of one of two cryoprotectants known to be used to maintain the integrity of the quiescent probiotics during drying of the quiescent probiotic powders.ResultsIt was found that topical treatment on Epiderm® tissues with creams containing 2 M (1%), 4 M (2%) and 6 M (3%) CFU/gram prepared from a base powder containing 200 M CFU/gram of Lactobacillus plantarum Lp90 stimulated elastin expression in a dose dependent fashion. There was no effect on the other biomolecules examined in the studies. In addition, it was found that creams made from powders containing only the known cryoprotectants, not bacteria, had no influence on elastin expression.ConclusionThe results of this study demonstrate that topical delivery of probiotics is possible from powders containing quiescent probiotic powders converted to creams just prior to application to the tissues. In the case of a powder containing Lactobacillus plantarum Lp90, topical application significantly increased expression of elastin in the skin replicants after 48 h of exposure to the cream made with the probiotic. The elastin‐stimulating effects are not coming from the oligosaccharide cryoprotectants used to maintain the probiotic powders in their quiescent, dried state. The results indicate that it is the living Lactobacillus plantarum probiotic that is stimulating the elastin expression in the skin tissues.

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Sucrose, maltodextrin and inulin efficacy as cryoprotectant, preservative and prebiotic – towards a freeze dried Lactobacillus plantarum topical probiotic

Cited by 44 publications

References 34 publications

Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics

Preparation and Evaluation of a Dosage Form for Individualized Administration of Lyophilized Probiotics

Kinetics of Cucurbita Moschata Puree Drying with Maltodextrin as Drying Aid

Living, quiescent Lactobacillus plantarum Lp90 probiotic, delivered topically to full thickness tissues in vitro via a just‐add‐water cream delivery system, stimulates the expression of elastin protein

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